##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088340_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3064073
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.023377706732184	31.0	31.0	33.0	30.0	34.0
2	31.33119119551003	31.0	31.0	34.0	30.0	34.0
3	31.397108032347795	31.0	31.0	34.0	28.0	34.0
4	35.27774273001982	37.0	35.0	37.0	33.0	37.0
5	35.04490330354401	35.0	35.0	37.0	32.0	37.0
6	35.10152662811885	37.0	35.0	37.0	32.0	37.0
7	34.911383638705736	36.0	35.0	37.0	32.0	37.0
8	34.98391781135763	36.0	35.0	37.0	32.0	37.0
9	36.46663803375442	38.0	35.0	39.0	32.0	39.0
10	36.279043612864314	38.0	35.0	39.0	32.0	39.0
11	36.3833041836797	38.0	35.0	39.0	32.0	39.0
12	36.14165067216088	38.0	35.0	39.0	31.0	39.0
13	36.21732543578433	38.0	35.0	39.0	32.0	39.0
14	37.042002915726876	39.0	36.0	40.0	31.0	41.0
15	37.17208956836211	39.0	36.0	40.0	31.0	41.0
16	37.14815182275357	39.0	36.0	40.0	31.0	41.0
17	37.237130446957366	39.0	36.0	40.0	32.0	41.0
18	37.247340386472516	39.0	36.0	40.0	32.0	41.0
19	37.342603129886264	39.0	36.0	41.0	32.0	41.0
20	37.327298990591935	39.0	36.0	41.0	31.0	41.0
21	37.252841560889706	39.0	36.0	40.0	31.0	41.0
22	37.17561330947402	39.0	36.0	40.0	31.0	41.0
23	37.054556794175596	39.0	36.0	40.0	31.0	41.0
24	36.96580792951082	39.0	36.0	40.0	31.0	41.0
25	36.8239102005729	39.0	35.0	40.0	30.0	41.0
26	36.60175916174321	38.0	35.0	40.0	30.0	41.0
27	36.46745981574199	38.0	35.0	40.0	30.0	41.0
28	36.30367194254184	38.0	35.0	40.0	30.0	41.0
29	36.082611935159505	38.0	35.0	40.0	30.0	41.0
30	35.846550653329736	38.0	34.0	40.0	29.0	41.0
31	35.58724971630898	38.0	34.0	40.0	28.0	41.0
32	35.465021557906745	38.0	34.0	40.0	27.0	41.0
33	35.27927533058122	38.0	34.0	40.0	27.0	41.0
34	35.41736113989451	38.0	34.0	40.0	27.0	41.0
35	35.32126682360374	38.0	34.0	40.0	27.0	41.0
36	35.24496674850762	38.0	34.0	40.0	26.0	41.0
37	35.123930467714054	38.0	34.0	40.0	26.0	41.0
38	35.08164133165235	38.0	34.0	40.0	25.0	41.0
39	34.960290436944554	38.0	33.0	40.0	25.0	41.0
40	34.74342093024546	38.0	33.0	40.0	24.0	41.0
41	34.60192136414504	38.0	33.0	40.0	23.0	41.0
42	34.424820165838085	38.0	33.0	40.0	23.0	41.0
43	34.04391018099112	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	1.0
14	7.0
15	11.0
16	28.0
17	105.0
18	225.0
19	546.0
20	1232.0
21	2368.0
22	4242.0
23	7367.0
24	12254.0
25	19219.0
26	29328.0
27	42403.0
28	59377.0
29	80072.0
30	103141.0
31	125579.0
32	153154.0
33	186388.0
34	224569.0
35	269325.0
36	331348.0
37	425376.0
38	506251.0
39	480154.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.49684847586856	22.40707058872292	14.516005330160214	22.58007560524831
2	18.344177831272297	23.866598478561052	35.89483670917762	21.89438698098903
3	19.55266731569385	25.293457433944948	33.54433787967845	21.609537370682748
4	13.32993698257189	16.523659847529743	36.50108858372499	33.64531458617337
5	12.019752793095986	38.610959986919376	35.51521128902608	13.854075930958565
6	31.334240404846753	37.31973748667215	13.660999591067183	17.685022517413913
7	24.24886743886324	34.4758757379475	22.071797897765492	19.203458925423774
8	26.150747713908906	34.61373146135879	19.65602647195416	19.57949435277815
9	23.75060907491434	14.593059630106724	23.488735418509936	38.167595876469
10	15.968418507000322	27.683380911616663	34.34262173257622	22.0055788488068
11	31.808967997825118	22.571426986236947	24.99806629933425	20.621538716603684
12	20.85655269962563	27.353591118749453	31.715791366589503	20.07406481503541
13	31.336459673121364	21.485943709565667	27.822509450656042	19.355087166656933
14	19.913853227387207	23.653940359776023	28.94046584399262	27.491740568844147
15	21.30366998436395	29.789956048697274	27.293605602738573	21.6127683642002
16	20.831944930815943	29.101558611691043	28.062125151717993	22.004371305775024
17	20.665989354692268	26.881539702219886	27.887879955862672	24.564590987225174
18	22.771226403548482	25.38164071156268	31.25571747148322	20.59141541340562
19	21.833324467139	24.720102947938905	32.68737396269606	20.759198622226037
20	23.11279790005003	23.96790154803753	32.78329204297678	20.136008508935653
21	21.80985896876478	24.990494678162044	33.436997095043104	19.76264925803008
22	19.87302521839395	25.287060719506353	32.885933200677655	21.953980861422036
23	21.341462817628692	25.59880263949325	34.149219029703275	18.910515513174783
24	21.18986721269369	25.472728619716307	31.301473561498046	22.03593060609196
25	20.8982618886691	26.287689620971822	32.36675496961071	20.447293520748364
26	20.66080018328545	27.063552337036356	32.08908534489876	20.186562134779425
27	19.09778259199438	27.22360074319378	31.85482199673441	21.823794668077426
28	20.09691022374467	26.43974213408101	31.895193097553488	21.568154544620835
29	20.234765947155957	26.307140854672852	30.974294672483328	22.483798525687867
30	20.650356567875505	26.490361032521093	33.05293313834233	19.806349261261072
31	21.751407358767235	25.99399557386524	32.65705484170906	19.59754222565846
32	21.094797676165026	26.226300744140236	31.29125840017519	21.387643179519547
33	20.545855141179732	26.147940992267483	32.15158385586766	21.154620010685125
34	19.46102459047157	27.030263312917153	32.93544246498043	20.57326963163084
35	19.107018664372553	29.42188387809298	31.299058475434492	20.17203898209997
36	21.863480406635222	26.611343789785685	30.89006038694248	20.635115416636616
37	20.88752454657575	27.543436465123385	30.826941786308616	20.742097201992248
38	20.56984282032445	25.655785616073768	32.36058670925921	21.41378485434257
39	20.125140621649678	25.3533124047632	33.14865540083412	21.372891572753
40	18.566985838783868	25.954864652376102	32.72154416686547	22.756605341974552
41	20.608125198061533	25.069931427873943	32.61508456228034	21.706858811784183
42	18.96544240297147	27.644152081233052	31.8311280442731	21.559277471522382
43	19.39141136650465	27.803547761427357	30.607071045631095	22.197969826436903
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	278.0
1	387.5
2	497.0
3	1392.5
4	2288.0
5	2288.0
6	3838.5
7	5389.0
8	5965.0
9	6541.0
10	10342.0
11	14143.0
12	14143.0
13	26066.0
14	37989.0
15	61985.0
16	85981.0
17	84110.0
18	82239.0
19	82239.0
20	87498.5
21	92758.0
22	76091.0
23	59424.0
24	65440.0
25	71456.0
26	71456.0
27	74825.0
28	78194.0
29	81512.0
30	84830.0
31	91562.5
32	98295.0
33	98295.0
34	106675.5
35	115056.0
36	125372.0
37	135688.0
38	141028.0
39	146368.0
40	146368.0
41	152270.0
42	158172.0
43	165049.0
44	171926.0
45	188573.0
46	205220.0
47	205220.0
48	284124.5
49	363029.0
50	329589.0
51	296149.0
52	261383.0
53	226617.0
54	226617.0
55	192711.5
56	158806.0
57	129738.5
58	100671.0
59	88272.5
60	75874.0
61	75874.0
62	66761.0
63	57648.0
64	50465.0
65	43282.0
66	36859.5
67	30437.0
68	30437.0
69	25908.5
70	21380.0
71	18109.0
72	14838.0
73	12395.5
74	9953.0
75	9953.0
76	8032.5
77	6112.0
78	4637.5
79	3163.0
80	2409.0
81	1655.0
82	1655.0
83	1253.0
84	851.0
85	665.0
86	479.0
87	367.5
88	256.0
89	256.0
90	181.5
91	107.0
92	64.0
93	21.0
94	14.5
95	8.0
96	8.0
97	6.0
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3064073.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.99650712658455
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.73775524406113	28.365183907467923
2	13.569063972416076	10.582921998028102
3	5.35520188342902	6.265025052343162
4	2.601162655335301	4.057450321047546
5	1.457529325513142	2.8419276364789603
6	0.8735970354932022	2.044033981222428
7	0.6090184810549517	1.6624715475676783
8	0.4226699110104722	1.3186120157530186
9	0.3063283237229349	1.0751161193222514
>10	1.7641556075865317	12.886778427275939
>50	0.16839186048933646	4.571665859779214
>100	0.11852673610587286	8.743794713799298
>500	0.008341611240732353	2.2071670563267176
>1k	0.006824954651501345	5.614372152206144
>5k	5.898108958087582E-4	1.3260154187702269
>10k+	8.425869940125117E-4	6.437463792611391
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	41157	1.3432121232098582	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	39310	1.2829328805155753	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	34823	1.1364938106892362	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14131	0.46118352924359174	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	11602	0.3786463312068609	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	11337	0.36999771219549926	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	11233	0.36660353718726674	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	11222	0.36624453790754985	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	10899	0.35570301360313544	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	10204	0.3330207863846586	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	6978	0.2277360885331387	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	6236	0.20351995530132602	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	5921	0.19323952138216027	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	5812	0.18968216488314738	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5225	0.17052465786552734	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	5114	0.16690202877020227	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	5070	0.1654660316513347	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	4520	0.1475160676654897	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	4457	0.14545998088165654	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	4143	0.13521218326064685	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3963	0.12933764959255212	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3916	0.12780374357921628	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3748	0.12232084548899455	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	3691	0.12046057649409789	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG	3533	0.11530404138543697	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	3497	0.11412913465181802	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3205	0.10459933559024213	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	3089	0.10081352500413666	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	3.263629815608179E-5	0.0	0.0	0.0	0.0
7	3.263629815608179E-5	0.0	0.0	3.263629815608179E-5	0.0
8	3.263629815608179E-5	0.0	0.0	3.263629815608179E-5	0.0
9	3.263629815608179E-5	0.0	0.0	3.263629815608179E-5	0.0
10	3.263629815608179E-5	0.0	0.0	9.790889446824537E-5	0.0
11	3.263629815608179E-5	0.0	0.0	3.916355778729815E-4	0.0
12	3.263629815608179E-5	0.0	0.0	0.0010117252428385355	0.0
13	3.263629815608179E-5	0.0	0.0	0.0012075430317750263	0.0
14	6.527259631216358E-5	0.0	0.0	0.0013054519262432715	0.0
15	6.527259631216358E-5	0.0	0.0	0.0016318149078040896	0.0
16	6.527259631216358E-5	0.0	0.0	0.002382449765393971	0.0
17	6.527259631216358E-5	0.0	0.0	0.003524720200856833	0.0
18	9.790889446824537E-5	0.0	0.0	0.004242718760290633	0.0
19	9.790889446824537E-5	0.0	0.0	0.005319716599441332	0.0
20	9.790889446824537E-5	0.0	0.0	0.008485437520581266	0.0
21	9.790889446824537E-5	0.0	0.0	0.014849515661017214	0.0
22	9.790889446824537E-5	0.0	0.0	0.023955042846564033	0.0
23	9.790889446824537E-5	0.0	0.0	0.02989484911097092	0.0
24	9.790889446824537E-5	0.0	0.0	0.03896773999836166	0.0
25	9.790889446824537E-5	0.0	0.0	0.04657199746872871	0.0
26	9.790889446824537E-5	0.0	0.0	0.05832106480491816	0.0
27	9.790889446824537E-5	0.0	0.0	0.14451352823513017	0.0
28	9.790889446824537E-5	0.0	0.0	0.2554443056676522	0.0
29	9.790889446824537E-5	0.0	0.0	0.37499106581337976	0.0
30	1.3054519262432717E-4	0.0	0.0	0.5855604615164195	0.0
31	1.3054519262432717E-4	0.0	0.0	0.8734778838493731	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9835	0.0	29.663956	1
ATTGGAC	1020	0.0	23.578432	3
GTATTGG	1005	0.0	23.378109	1
TTGGACC	1805	0.0	19.166204	4
TGGACCC	1860	0.0	18.698925	5
GGACCCT	1810	0.0	18.60221	6
ACGTTTA	195	0.0	18.025642	26
GACCCTC	1875	0.0	17.562668	7
TATACCG	255	0.0	17.411764	5
GTATCAA	17115	0.0	17.132633	2
CAATGCG	220	0.0	16.818182	19
TATTGGA	1475	0.0	16.305084	2
GTAGTGT	175	1.3278623E-10	15.857143	6
CTGTGCG	280	0.0	15.857142	9
CGTGTCT	345	0.0	15.550725	35
CGACGAG	250	0.0	15.540001	24
GACCGTG	265	0.0	15.358491	7
CGAATTA	265	0.0	15.358491	15
CGGTATA	230	0.0	15.282607	26
CCGACCG	280	0.0	15.196428	9
>>END_MODULE