FastQCFastQC Report
Wed 25 May 2016
SRR2088339_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088339_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1016174
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT114461.1263818991629386No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT109191.0745207021632124No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT94750.9324190542170927No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39950.3931413320947003No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG31240.30742766494714485No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA29110.28646668779165774No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA29050.2858762377309398No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG28770.28312080411425605No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG28440.2798733287803073No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA25670.2526142176438287No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA18170.17880796005408522No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA15450.15204089063487158No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA14460.14229846463302545No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT14310.14082233948123057No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC14040.13816531420799982No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA13390.13176877188355537No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA12400.12202634588170923No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11690.11503935349654686No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG11580.11395686171856395No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG11450.11267755325367505No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG10870.10696986933340157No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA10370.10204945216075201No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28700.030.2961671
GCCGTCA1107.4578566E-1121.86363613
AGCCGTC957.1431714E-921.42105312
CGTTTAT909.4756615E-820.5555571
GACCGTC555.1425304E-420.18181822
CGGTGTG555.1425304E-420.1818187
GTATTGG2500.019.981
GGCGATT500.00703433518.58
CTTACTC701.21902114E-418.53
ATTGGAC2900.017.8620683
GTATCAA49000.017.7826542
TAAGCCG1054.795911E-717.61904710
GTGCGGT650.00157976417.07692311
ACGGGCG1005.8764417E-616.6500025
GCGTCGG700.00259221715.8571429
GGACCCT5150.015.8058266
TTGGACC5200.015.6538464
TTTACAC1201.9348645E-615.4166663
ATACCGT855.36339E-415.2352946
CTGTGCG855.36339E-415.2352949
Produced by FastQC (version 0.11.5)