##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088339_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1016174
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.98759169197401	31.0	31.0	33.0	28.0	34.0
2	31.303193153928362	31.0	31.0	34.0	28.0	34.0
3	31.378581817680832	31.0	31.0	34.0	28.0	34.0
4	35.26217754046059	35.0	35.0	37.0	33.0	37.0
5	35.01372304349452	35.0	35.0	37.0	32.0	37.0
6	35.066207165308306	36.0	35.0	37.0	32.0	37.0
7	34.887380507668965	36.0	35.0	37.0	32.0	37.0
8	34.94934135295727	36.0	35.0	37.0	32.0	37.0
9	36.43778329301872	38.0	35.0	39.0	32.0	39.0
10	36.23312444522296	38.0	35.0	39.0	32.0	39.0
11	36.34423238539856	38.0	35.0	39.0	32.0	39.0
12	36.105265436824794	38.0	35.0	39.0	31.0	39.0
13	36.1786829814579	38.0	35.0	39.0	32.0	39.0
14	37.02059194586754	39.0	36.0	40.0	31.0	41.0
15	37.14391728188283	39.0	36.0	40.0	31.0	41.0
16	37.125825892022426	39.0	36.0	40.0	31.0	41.0
17	37.18692468022209	39.0	36.0	40.0	31.0	41.0
18	37.18842737562662	39.0	36.0	40.0	31.0	41.0
19	37.28048838092689	39.0	36.0	40.0	31.0	41.0
20	37.258555129338085	39.0	36.0	40.0	31.0	41.0
21	37.183620128048936	39.0	36.0	40.0	31.0	41.0
22	37.11638065921781	39.0	36.0	40.0	31.0	41.0
23	37.00035131778613	39.0	36.0	40.0	31.0	41.0
24	36.913305201668216	39.0	35.0	40.0	31.0	41.0
25	36.77737474094003	39.0	35.0	40.0	30.0	41.0
26	36.54770836490601	38.0	35.0	40.0	30.0	41.0
27	36.423013184749855	38.0	35.0	40.0	30.0	41.0
28	36.27701259823613	38.0	35.0	40.0	30.0	41.0
29	36.05091844506945	38.0	35.0	40.0	29.0	41.0
30	35.83675728762987	38.0	34.0	40.0	29.0	41.0
31	35.61692387327367	38.0	34.0	40.0	28.0	41.0
32	35.49434348841832	38.0	34.0	40.0	27.0	41.0
33	35.329672870984695	38.0	34.0	40.0	27.0	41.0
34	35.5024680812538	38.0	34.0	40.0	27.0	41.0
35	35.42851027481514	38.0	34.0	40.0	27.0	41.0
36	35.37871663711136	38.0	34.0	40.0	27.0	41.0
37	35.275256009305494	38.0	34.0	40.0	26.0	41.0
38	35.255272227000496	38.0	34.0	40.0	26.0	41.0
39	35.15411140218112	38.0	34.0	40.0	25.0	41.0
40	34.96269930149758	38.0	33.0	40.0	25.0	41.0
41	34.83409731010634	38.0	33.0	40.0	24.0	41.0
42	34.70453091694926	38.0	33.0	40.0	24.0	41.0
43	34.33845187930414	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	4.0
16	14.0
17	34.0
18	86.0
19	160.0
20	390.0
21	773.0
22	1301.0
23	2336.0
24	3911.0
25	6227.0
26	9475.0
27	13792.0
28	19270.0
29	26166.0
30	33633.0
31	41568.0
32	50715.0
33	61924.0
34	75162.0
35	89935.0
36	110955.0
37	140310.0
38	170610.0
39	157423.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.030644358151264	22.680269323954363	14.319299647501312	22.969786670393063
2	18.262128336288864	23.91283382570308	35.867479388372466	21.957558449635595
3	19.442339599320587	25.450661008842975	33.15367250096932	21.953326890867114
4	13.540594425757794	16.888938311745825	36.84998828940713	32.72047897308926
5	12.197320537624462	38.536805704534856	35.73285677452877	13.533016983311914
6	31.259213481155783	37.84617595018176	14.24450930647704	16.650101262185412
7	24.543729715580206	34.14031455242901	22.515533757014055	18.80042197497673
8	25.78515096823969	35.044392003731645	19.91214103096517	19.258315997063495
9	24.059757482478396	14.735763756994372	23.106279042762363	38.09819971776487
10	15.714631549321279	27.72901097646663	34.888808412732466	21.66754906147963
11	32.04470887859756	22.857502750513202	24.617437564826496	20.48035080606274
12	20.622649270695767	27.42768462881357	31.73432896334683	20.215337137143834
13	30.68716578066355	21.733974693310397	27.408888635214048	20.169970890812007
14	20.01615864999498	23.527466752741162	28.771450558664164	27.68492403859969
15	21.6106690389638	30.07083432561746	26.641106739593813	21.677389895824927
16	21.0349802297638	28.914142656671004	27.816495993796337	22.23438111976886
17	20.80539356448797	27.307232816427106	28.027089848785742	23.86028377029918
18	22.318815478451526	26.0321559103067	30.791478624723716	20.857549986518055
19	21.876962016347594	25.437769515850633	31.995898340244878	20.689370127556895
20	22.777103133912107	25.003985537909845	31.81964899712057	20.39926233105748
21	21.74135531906937	25.726696412228616	32.36168215285965	20.170266115842367
22	20.277432801862673	26.109012826543488	31.869443618907784	21.744110752686055
23	21.403519475995253	26.220411071332272	32.84427666915312	19.531792783519357
24	21.298419365187456	26.08893752447908	30.656265560819307	21.95637754951416
25	21.116462338142878	26.782716345822667	31.39816606211141	20.702655253923048
26	20.894256298626026	27.355846538092887	31.30054498540604	20.44935217787505
27	19.745142072125443	27.52569933889275	31.007288121916126	21.721870467065678
28	20.454272595047698	26.978155315920304	31.01722736460488	21.550344724427113
29	20.646562498154843	26.762050593697534	30.369897281371106	22.221489626776517
30	20.895634015434364	27.124980564352168	31.913136923400913	20.066248496812552
31	21.822443794074637	26.599184785282837	31.688273858610827	19.8900975620317
32	21.04511628913946	26.83359345938786	30.758807054697325	21.362483196775354
33	20.805984014548688	26.734397849187246	31.272695424208845	21.18692271205522
34	19.942647617435597	27.212465581681876	32.082300865796604	20.76258593508592
35	19.71040392688654	29.195492110603105	30.70202544052495	20.392078521985407
36	21.807288909182876	26.99567200105494	30.387906008223002	20.809133081539187
37	20.934800536128655	27.699980515148	30.451280981406725	20.91393796731662
38	20.78069307028127	26.246784507377676	31.559162112000504	21.41336031034055
39	20.340217324985684	25.88228000322779	32.34268934257322	21.434813329213302
40	19.23184415267464	26.46239718788318	31.912251248309836	22.393507411132347
41	20.683170401919355	25.61795519271306	32.0327030606963	21.666171344671287
42	19.397465394706025	27.694764872944987	31.238744545717562	21.669025186631423
43	19.769547341301784	27.65736970243285	30.482378017937872	22.09070493832749
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	89.0
1	129.5
2	170.0
3	433.0
4	696.0
5	696.0
6	1149.5
7	1603.0
8	1899.5
9	2196.0
10	3208.5
11	4221.0
12	4221.0
13	7698.5
14	11176.0
15	17777.5
16	24379.0
17	23975.5
18	23572.0
19	23572.0
20	25374.0
21	27176.0
22	22865.5
23	18555.0
24	20693.0
25	22831.0
26	22831.0
27	25223.0
28	27615.0
29	29427.0
30	31239.0
31	33878.0
32	36517.0
33	36517.0
34	39735.5
35	42954.0
36	46910.5
37	50867.0
38	52980.0
39	55093.0
40	55093.0
41	56240.0
42	57387.0
43	59215.0
44	61043.0
45	64743.0
46	68443.0
47	68443.0
48	88236.5
49	108030.0
50	98369.0
51	88708.0
52	79312.5
53	69917.0
54	69917.0
55	60910.0
56	51903.0
57	43649.5
58	35396.0
59	31418.5
60	27441.0
61	27441.0
62	24278.0
63	21115.0
64	18093.0
65	15071.0
66	12735.0
67	10399.0
68	10399.0
69	8964.0
70	7529.0
71	6405.5
72	5282.0
73	4337.0
74	3392.0
75	3392.0
76	2652.5
77	1913.0
78	1484.5
79	1056.0
80	838.0
81	620.0
82	620.0
83	448.0
84	276.0
85	223.5
86	171.0
87	128.5
88	86.0
89	86.0
90	60.0
91	34.0
92	22.0
93	10.0
94	6.5
95	3.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1016174.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.48300862773448
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.4470682790734	44.37481323752435
2	10.87206393543831	11.846855063915326
3	3.2175297126157845	5.2590209727731345
4	1.3525836091858365	2.9477129779601667
5	0.7323547681777427	1.9950445576595217
6	0.4132029972042552	1.3507525477011106
7	0.2979789043766064	1.136435105262346
8	0.22901715701847009	0.998203499338922
9	0.19024816781644702	0.9328763311698742
>10	1.0871881442682065	11.225320825017642
>50	0.10027778750868169	3.7403945027217333
>100	0.049588062371754255	4.84337847903882
>500	0.00690236746488246	2.5324789694073164
>1k	0.00345118373244123	3.6657113523179525
>5k	1.8164124907585423E-4	0.9376802749173241
>10k+	3.6328249815170845E-4	2.2133213032745065
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	11446	1.1263818991629386	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	10919	1.0745207021632124	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9475	0.9324190542170927	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3995	0.3931413320947003	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	3124	0.30742766494714485	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	2911	0.28646668779165774	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	2905	0.2858762377309398	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	2877	0.28312080411425605	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	2844	0.2798733287803073	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2567	0.2526142176438287	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	1817	0.17880796005408522	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1545	0.15204089063487158	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1446	0.14229846463302545	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1431	0.14082233948123057	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	1404	0.13816531420799982	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1339	0.13176877188355537	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	1240	0.12202634588170923	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1169	0.11503935349654686	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	1158	0.11395686171856395	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	1145	0.11267755325367505	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	1087	0.10696986933340157	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1037	0.10204945216075201	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	9.840834345299132E-5	0.0
10	0.0	0.0	0.0	1.9681668690598264E-4	0.0
11	0.0	0.0	0.0	3.9363337381196527E-4	0.0
12	0.0	0.0	0.0	8.856750910769219E-4	0.0
13	0.0	0.0	0.0	9.84083434529913E-4	0.0
14	0.0	0.0	0.0	0.0013777168083418784	0.0
15	0.0	0.0	0.0	0.001869758525606835	0.0
16	0.0	0.0	0.0	0.0026570252732307656	0.0
17	0.0	0.0	0.0	0.0036411087077606787	0.0
18	0.0	0.0	0.0	0.004822008829196574	0.0
19	0.0	0.0	0.0	0.006002908950632471	0.0
20	0.0	0.0	0.0	0.008364709193504263	0.0
21	0.0	0.0	0.0	0.013973984770324768	0.0
22	0.0	0.0	0.0	0.021059385498940143	0.0
23	0.0	0.0	0.0	0.02538935261087176	0.0
24	0.0	0.0	0.0	0.03276997836984611	0.0
25	0.0	0.0	0.0	0.03808402891630764	0.0
26	0.0	0.0	0.0	0.04851531332232472	0.0
27	0.0	0.0	0.0	0.12478177949839299	0.0
28	0.0	0.0	0.0	0.22387898135555526	0.0
29	0.0	0.0	0.0	0.328782275476444	0.0
30	0.0	0.0	0.0	0.5058188853483754	0.0
31	0.0	0.0	0.0	0.7620742116999648	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2870	0.0	30.296167	1
GCCGTCA	110	7.4578566E-11	21.863636	13
AGCCGTC	95	7.1431714E-9	21.421053	12
CGTTTAT	90	9.4756615E-8	20.555557	1
GACCGTC	55	5.1425304E-4	20.181818	22
CGGTGTG	55	5.1425304E-4	20.181818	7
GTATTGG	250	0.0	19.98	1
GGCGATT	50	0.007034335	18.5	8
CTTACTC	70	1.21902114E-4	18.5	3
ATTGGAC	290	0.0	17.862068	3
GTATCAA	4900	0.0	17.782654	2
TAAGCCG	105	4.795911E-7	17.619047	10
GTGCGGT	65	0.001579764	17.076923	11
ACGGGCG	100	5.8764417E-6	16.650002	5
GCGTCGG	70	0.002592217	15.857142	9
GGACCCT	515	0.0	15.805826	6
TTGGACC	520	0.0	15.653846	4
TTTACAC	120	1.9348645E-6	15.416666	3
ATACCGT	85	5.36339E-4	15.235294	6
CTGTGCG	85	5.36339E-4	15.235294	9
>>END_MODULE