FastQCFastQC Report
Wed 25 May 2016
SRR2088338_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088338_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465431
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT32620.7008557659459727No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT32330.6946249820059257No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT25730.5528209337152017No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12260.2634117624309511No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG6500.139655502104501No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA6360.13664753744378866No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG6070.13041675350374168No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG5380.11559178481880235No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA4870.10463419926906459No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATCGT250.005493025329.59999814
GGTATCA8750.024.5257151
GCTCCGT400.001929469923.12500229
GTCTTAT603.720157E-521.5833321
AGCGAAC450.00382243920.55555522
ACGGGAT555.1367766E-420.1818185
ATTGGAC555.1367766E-420.1818183
CGAACTA500.007028747318.49999824
TAGTCCC500.007028747318.4999985
GATACCC1258.540155E-917.7636
ACGTCTT650.001578025217.0769217
ATAGTAT1452.9631337E-916.5862066
TAGGAGT803.3771576E-416.18754
CTATAGT700.00258938615.8571434
GCTTTAG700.00258938615.8571431
GTATTGG957.0452545E-515.5789481
TTATGCG855.3556374E-415.2352944
GTTATAT855.3556374E-415.2352941
GTATCAA14600.015.205481
GGCATAT750.00409952714.81
Produced by FastQC (version 0.11.5)