##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088338_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	465431
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.941245855991543	31.0	31.0	33.0	28.0	34.0
2	31.297648845908416	31.0	31.0	34.0	28.0	34.0
3	31.388962058822898	31.0	31.0	34.0	28.0	34.0
4	35.27443165582009	35.0	35.0	37.0	33.0	37.0
5	34.9980190404163	35.0	35.0	37.0	32.0	37.0
6	35.02044341696191	36.0	35.0	37.0	32.0	37.0
7	34.86365970466084	36.0	35.0	37.0	32.0	37.0
8	34.88829923232445	36.0	35.0	37.0	32.0	37.0
9	36.41384222365936	38.0	35.0	39.0	32.0	39.0
10	36.18289714264843	38.0	35.0	39.0	32.0	39.0
11	36.28025851307713	38.0	35.0	39.0	32.0	39.0
12	36.04674377082747	38.0	35.0	39.0	30.0	39.0
13	36.137339369315754	38.0	35.0	39.0	31.0	39.0
14	37.0087488800703	39.0	36.0	40.0	31.0	41.0
15	37.145226682365376	39.0	36.0	40.0	31.0	41.0
16	37.132565299689965	39.0	36.0	40.0	31.0	41.0
17	37.145314772759015	39.0	36.0	40.0	31.0	41.0
18	37.13299286038102	39.0	36.0	40.0	31.0	41.0
19	37.212927372693265	39.0	36.0	40.0	31.0	41.0
20	37.18277897260818	39.0	36.0	40.0	31.0	41.0
21	37.10885824107118	39.0	36.0	40.0	31.0	41.0
22	37.035672312329865	39.0	36.0	40.0	31.0	41.0
23	36.92462685124111	39.0	36.0	40.0	31.0	41.0
24	36.849636143703364	39.0	35.0	40.0	30.0	41.0
25	36.72189862729384	38.0	35.0	40.0	30.0	41.0
26	36.50637366226143	38.0	35.0	40.0	30.0	41.0
27	36.3709959156137	38.0	35.0	40.0	30.0	41.0
28	36.24285447252117	38.0	35.0	40.0	30.0	41.0
29	36.04795125378413	38.0	34.0	40.0	29.0	41.0
30	35.8686099550739	38.0	34.0	40.0	29.0	41.0
31	35.72541150030832	38.0	34.0	40.0	28.0	41.0
32	35.58035885018402	38.0	34.0	40.0	27.0	41.0
33	35.426754986238564	38.0	34.0	40.0	27.0	41.0
34	35.632065762701664	38.0	34.0	40.0	28.0	41.0
35	35.60341060221601	38.0	34.0	40.0	27.0	41.0
36	35.579039642825684	38.0	34.0	40.0	27.0	41.0
37	35.508829450552284	38.0	34.0	40.0	27.0	41.0
38	35.52072165369303	38.0	34.0	40.0	27.0	41.0
39	35.47112031643788	38.0	34.0	40.0	27.0	41.0
40	35.33399150464838	38.0	34.0	40.0	26.0	41.0
41	35.27445743837433	38.0	34.0	40.0	26.0	41.0
42	35.198267842064666	38.0	34.0	40.0	25.0	41.0
43	34.85260113744035	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	0.0
15	2.0
16	2.0
17	11.0
18	26.0
19	91.0
20	154.0
21	332.0
22	617.0
23	1081.0
24	1785.0
25	2810.0
26	4459.0
27	6052.0
28	8753.0
29	11758.0
30	15246.0
31	18801.0
32	23032.0
33	28066.0
34	33725.0
35	40599.0
36	49745.0
37	62037.0
38	78563.0
39	77683.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.406915310754975	22.993956139578152	13.761438322758906	23.837690226907963
2	18.06712487994998	23.807180871063593	35.577131733812315	22.54856251517411
3	18.936426666895844	25.820583502173257	32.48236580717657	22.76062402375433
4	13.652721885736016	17.32995868345684	37.64166976415409	31.37564966665306
5	12.578663647243093	38.448234002462236	35.88286985611186	13.09023249418281
6	30.980532023006635	38.77373874967503	15.407439556024416	14.83828967129392
7	24.84320984206037	33.51667594122437	23.504665568043386	18.135448648671876
8	24.96137988230264	35.58851902859929	20.78568036937806	18.664420719720002
9	24.5651879655631	14.876104084171446	22.6110852091932	37.94762274107225
10	15.25790074146329	28.197735002610486	35.575198042244715	20.96916621368151
11	32.38954861193174	23.60736607574485	24.233452434410257	19.76963287791316
12	19.75008110761853	27.396756984386517	32.183503032672945	20.669658875322014
13	29.253745453139135	22.353904230702295	27.06695514480127	21.3253951713573
14	20.061620304620877	23.338582947848337	28.609181597272205	27.99061515025858
15	22.11627502250602	30.422984287681743	25.708859100489654	21.751881589322586
16	21.382546499910834	29.337753609020456	27.34648100362889	21.933218887439814
17	20.878927273860143	28.035949474787884	28.408292528860347	22.676830722491626
18	21.423583732067698	27.59893518051011	29.880046666423166	21.097434420999033
19	21.68097956517722	26.775612281949417	30.936057116951815	20.607351035921546
20	22.011640823236956	26.902806216173826	30.346066334214957	20.739486626374266
21	21.330121972966992	27.283743454991182	30.69112285172238	20.695011720319446
22	20.89804933491753	27.47453435632779	30.40901873747129	21.21839757128339
23	21.427880824440145	27.436719943450267	30.54502171105921	20.59037752105038
24	21.287580758479773	27.6315930825407	29.666481175512587	21.414344983466936
25	21.381472226817724	27.803476777438547	30.106933143688323	20.70811785205541
26	21.069933029815374	28.147673876471487	29.947725871289194	20.834667222423946
27	20.65612303434881	28.085580891689638	29.961046857643776	21.29724921631778
28	20.79771222802091	27.928951874713974	29.93633857650221	21.336997320762908
29	20.883439220851212	27.86256179755968	29.78013926876379	21.473859712825316
30	21.11934959209851	28.052708135040426	30.378294527008297	20.44964774585277
31	21.536167552225784	27.816797763793126	30.208989087533922	20.438045596447164
32	21.032548326175093	27.88275813171018	29.786370052703838	21.29832348941089
33	20.90084244495962	27.896723681920626	30.071052422378397	21.131381450741358
34	20.681905588583486	27.78156160633907	30.64428454486272	20.89224826021473
35	20.642802047994223	28.5320487891868	30.005736618317215	20.819412544501763
36	21.53466356989543	27.882543277091553	29.65853155462356	20.92426159838945
37	21.071651866764356	27.65501223597053	29.91764622468207	21.355689672583047
38	20.826502746916297	27.149029609114994	30.571233974531133	21.453233669437573
39	20.591666648762118	26.864132384821808	31.21021161031388	21.333989356102194
40	19.866102601674577	26.91634205714703	31.207848209509038	22.009707131669355
41	20.56309098448535	26.22279134823422	31.293575202339337	21.920542464941096
42	19.550266312299783	27.50611798526527	30.934553134621456	22.00906256781349
43	20.074941290975463	27.123891618736184	30.441891494120505	22.35927559616785
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	53.0
1	76.5
2	100.0
3	251.5
4	403.0
5	403.0
6	582.5
7	762.0
8	819.0
9	876.0
10	1238.0
11	1600.0
12	1600.0
13	2787.0
14	3974.0
15	6149.5
16	8325.0
17	8197.0
18	8069.0
19	8069.0
20	8995.5
21	9922.0
22	9346.0
23	8770.0
24	10297.0
25	11824.0
26	11824.0
27	13210.5
28	14597.0
29	15745.5
30	16894.0
31	18490.0
32	20086.0
33	20086.0
34	21748.5
35	23411.0
36	25182.0
37	26953.0
38	28017.0
39	29081.0
40	29081.0
41	29610.5
42	30140.0
43	30587.0
44	31034.0
45	31043.0
46	31052.0
47	31052.0
48	35100.5
49	39149.0
50	36023.0
51	32897.0
52	29950.0
53	27003.0
54	27003.0
55	24590.5
56	22178.0
57	19815.5
58	17453.0
59	15870.5
60	14288.0
61	14288.0
62	12473.0
63	10658.0
64	9317.0
65	7976.0
66	6751.0
67	5526.0
68	5526.0
69	4708.5
70	3891.0
71	3280.5
72	2670.0
73	2204.5
74	1739.0
75	1739.0
76	1386.0
77	1033.0
78	773.5
79	514.0
80	392.0
81	270.0
82	270.0
83	207.5
84	145.0
85	98.5
86	52.0
87	48.5
88	45.0
89	45.0
90	29.0
91	13.0
92	8.5
93	4.0
94	2.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	465431.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.03617699765095
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.04270191836424	56.15894916239571
2	9.234043141136638	12.195618145440879
3	2.3795867803592823	4.714164414272278
4	1.0212580096531036	2.6975989874288433
5	0.5405991133882155	1.7849549368238689
6	0.3269655765299617	1.2954934010322925
7	0.23014466897731778	1.0638511866956362
8	0.18345777336674338	0.9691879994912962
9	0.13757897656618362	0.8176670682912158
>10	0.8215029900505039	10.222028252554685
>50	0.053143854967621064	2.3327934522689278
>100	0.026408909301765857	3.0079794279837615
>500	0.0013041436692230051	0.5233983243453242
>1k	0.0013041436692230051	2.2163152409752227
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3262	0.7008557659459727	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3233	0.6946249820059257	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2573	0.5528209337152017	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1226	0.2634117624309511	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	650	0.139655502104501	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	636	0.13664753744378866	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	607	0.13041675350374168	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	538	0.11559178481880235	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	487	0.10463419926906459	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	2.1485461862230922E-4	0.0
11	0.0	0.0	0.0	2.1485461862230922E-4	0.0
12	0.0	0.0	0.0	2.1485461862230922E-4	0.0
13	0.0	0.0	0.0	2.1485461862230922E-4	0.0
14	0.0	0.0	0.0	2.1485461862230922E-4	0.0
15	0.0	0.0	0.0	2.1485461862230922E-4	0.0
16	0.0	0.0	0.0	2.1485461862230922E-4	0.0
17	0.0	0.0	0.0	4.2970923724461844E-4	0.0
18	0.0	0.0	0.0	4.2970923724461844E-4	0.0
19	0.0	0.0	0.0	8.594184744892369E-4	0.0
20	0.0	0.0	0.0	0.0017188369489784738	0.0
21	0.0	0.0	0.0	0.0036525285165792567	0.0
22	0.0	0.0	0.0	0.0051565108469354215	0.0
23	0.0	0.0	0.0	0.00816447550764775	0.0
24	0.0	0.0	0.0	0.011817004024227007	0.0
25	0.0	0.0	0.0	0.01675866025254012	0.0
26	0.0	0.0	0.0	0.027716245802277887	0.0
27	0.0	0.0	0.0	0.0816447550764775	0.0
28	0.0	0.0	0.0	0.1516873607473503	0.0
29	0.0	0.0	0.0	0.2262419134092916	0.0
30	0.0	0.0	0.0	0.3426931167025832	0.0
31	0.0	0.0	0.0	0.5465901497751546	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAATCGT	25	0.0054930253	29.599998	14
GGTATCA	875	0.0	24.525715	1
GCTCCGT	40	0.0019294699	23.125002	29
GTCTTAT	60	3.720157E-5	21.583332	1
AGCGAAC	45	0.003822439	20.555555	22
ACGGGAT	55	5.1367766E-4	20.181818	5
ATTGGAC	55	5.1367766E-4	20.181818	3
CGAACTA	50	0.0070287473	18.499998	24
TAGTCCC	50	0.0070287473	18.499998	5
GATACCC	125	8.540155E-9	17.76	36
ACGTCTT	65	0.0015780252	17.076921	7
ATAGTAT	145	2.9631337E-9	16.586206	6
TAGGAGT	80	3.3771576E-4	16.1875	4
CTATAGT	70	0.002589386	15.857143	4
GCTTTAG	70	0.002589386	15.857143	1
GTATTGG	95	7.0452545E-5	15.578948	1
TTATGCG	85	5.3556374E-4	15.235294	4
GTTATAT	85	5.3556374E-4	15.235294	1
GTATCAA	1460	0.0	15.20548	1
GGCATAT	75	0.004099527	14.8	1
>>END_MODULE