FastQCFastQC Report
Wed 25 May 2016
SRR2088337_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088337_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1768008
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT280851.5885109117153315No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT274101.5503323514373237No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT237641.3441115651060402No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95270.538855027805304No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA83860.4743191207279605No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG81030.45831240582621796No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA77920.44072198768331367No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG77020.43563151297957925No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG74510.4214347446391645No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA68320.3864235908434804No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA47440.26832457771684287No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA43590.24654865815086807No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA42280.2391391894154325No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC42080.23800797281460262No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA36560.20678639463169848No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA36560.20678639463169848No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT35840.20271401486871102No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG29750.16826846937344175No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG29630.16758973941294383No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG29560.16719381360265337No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA28960.16380016380016382No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25870.14632286731734245No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC25090.14191112257410599No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT24760.14004461518273673No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC24150.13659440455020566No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG23870.13501070130904386No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT22380.1265831376328614No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT22010.12449038692132613No Hit
ATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGGA20630.11668499237560011No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA20530.11611938407518518No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCCT19780.1118773218220732No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19750.11170763933194873No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG19720.11153795684182424No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA19530.11046330107103589No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA19100.10803118537925167No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT18960.10723933375867077No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT18590.10514658304713555No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC18180.10282758901543432No Hit
ACGCACATGCGAGGGTCCAAAAAAAAAAAAAAAAAAAAAAAAA17920.10135700743435551No Hit
GCCGAGGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAA17880.10113076411418953No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG17740.10033891249360864No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG17690.10005610834340115No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA68450.029.9459421
GTATTGG7450.021.1073821
ATTGGAC6950.021.0287763
GTCGTCA450.003826610320.55555524
CGTATAC656.904817E-519.9230773
TTGGACC12450.019.6144584
ACGTTTA1355.638867E-1119.18518626
ACCGTCC609.239494E-418.58
GGACCCT12750.017.847066
TGGACCC13300.017.804515
ACGGGCG1054.800877E-717.6190475
CTTAGAC2000.017.5753
TAATACT2650.017.4528314
GACCCTC13050.017.1532557
GTATCAA123250.016.6762682
GATACTG6500.016.50769236
CTAATAC1801.0913936E-1116.4444453
TCACGTT1504.6748028E-916.03333324
CGTTGAC1851.8189894E-1116.032
CAGACGT700.002593234215.8571424
Produced by FastQC (version 0.11.5)