##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088337_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1768008
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.89668711906281	31.0	31.0	33.0	28.0	34.0
2	31.212462273926363	31.0	31.0	34.0	28.0	34.0
3	31.263582517726164	31.0	31.0	34.0	28.0	34.0
4	35.158278129963215	35.0	35.0	37.0	33.0	37.0
5	34.92866604675997	35.0	35.0	37.0	32.0	37.0
6	34.98783263424147	36.0	35.0	37.0	32.0	37.0
7	34.775366401057006	35.0	35.0	37.0	32.0	37.0
8	34.85779872036778	35.0	35.0	37.0	32.0	37.0
9	36.3011213750164	38.0	35.0	39.0	32.0	39.0
10	36.12655768525934	38.0	35.0	39.0	31.0	39.0
11	36.223165845403415	38.0	35.0	39.0	32.0	39.0
12	35.96968678874756	38.0	35.0	39.0	30.0	39.0
13	36.04527015714861	38.0	35.0	39.0	30.0	39.0
14	36.80284138985797	38.0	36.0	40.0	31.0	41.0
15	36.94241089406835	38.0	36.0	40.0	31.0	41.0
16	36.9073058492948	38.0	36.0	40.0	31.0	41.0
17	37.03414520748775	38.0	36.0	40.0	31.0	41.0
18	37.055829498509055	39.0	36.0	40.0	31.0	41.0
19	37.1544461337279	39.0	36.0	40.0	31.0	41.0
20	37.14832512070081	39.0	36.0	40.0	31.0	41.0
21	37.07801774652604	39.0	36.0	40.0	31.0	41.0
22	37.00353165822779	39.0	36.0	40.0	31.0	41.0
23	36.8553716951507	39.0	35.0	40.0	31.0	41.0
24	36.76342754105185	39.0	35.0	40.0	30.0	41.0
25	36.62629128374985	38.0	35.0	40.0	30.0	41.0
26	36.36905602237094	38.0	35.0	40.0	30.0	41.0
27	36.24190105474636	38.0	35.0	40.0	30.0	41.0
28	36.06277234039665	38.0	34.0	40.0	30.0	41.0
29	35.825646150922395	38.0	34.0	40.0	29.0	41.0
30	35.562333993963826	38.0	34.0	40.0	27.0	41.0
31	35.26156046805218	37.0	34.0	40.0	27.0	41.0
32	35.13950559047244	37.0	33.0	40.0	27.0	41.0
33	34.93766487481957	37.0	33.0	40.0	26.0	41.0
34	35.03654508350641	37.0	33.0	40.0	26.0	41.0
35	34.91045063144511	37.0	33.0	40.0	25.0	41.0
36	34.81670162125963	37.0	33.0	40.0	25.0	41.0
37	34.66191499133488	37.0	33.0	40.0	24.0	41.0
38	34.58268627743766	37.0	33.0	40.0	24.0	41.0
39	34.43400425789928	37.0	33.0	40.0	23.0	41.0
40	34.19172933606635	37.0	33.0	40.0	22.0	41.0
41	34.02337885348935	37.0	33.0	40.0	21.0	41.0
42	33.793094262016915	37.0	33.0	40.0	20.0	41.0
43	33.39154008352904	37.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	3.0
15	10.0
16	27.0
17	69.0
18	175.0
19	400.0
20	800.0
21	1745.0
22	3017.0
23	5283.0
24	8594.0
25	13404.0
26	20150.0
27	28632.0
28	39378.0
29	51925.0
30	65311.0
31	78622.0
32	94602.0
33	112937.0
34	133028.0
35	157132.0
36	191535.0
37	242489.0
38	273467.0
39	245272.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.449873529984025	22.592318586793724	15.01961529585839	21.938192587363858
2	18.33436274044009	24.245478527246483	36.160696105447485	21.25946262686594
3	19.59595205451559	25.298245256808794	34.348713354238214	20.7570893344374
4	13.015156040017919	16.494156135040114	36.55294546178524	33.93774236315672
5	11.548533717042005	39.133250528278154	35.54859480273845	13.769620951941395
6	31.057664897443903	37.28620006244316	13.167361233659577	18.488773806453366
7	23.70068461228682	35.14209211723024	21.717944715182284	19.439278555300653
8	26.316792684196	34.42755915131605	19.47434626992638	19.78130189456156
9	23.400007239786245	14.640997099560634	24.007357432771798	37.951638227881325
10	16.00773299668327	27.784998710413074	34.19526382233565	22.012004470568005
11	31.071126374993778	22.587228112089992	25.422792204560164	20.91885330835607
12	21.24232469536337	27.59749955882553	31.866032280396922	19.294143465414184
13	31.953249080320905	21.44933733331523	28.19121859177108	18.406194994592784
14	19.740804340251856	23.962278451228727	29.499583712290896	26.797333496228525
15	20.81003027135624	30.14070072081122	27.936185809113983	21.113083198718556
16	20.31353930525201	29.17170058054036	28.650209727557797	21.864550386649835
17	20.36727209379143	26.812435237849602	28.0231763657178	24.797116302641165
18	22.75990832620667	24.954638214306723	32.06173275234049	20.22372070714612
19	21.591870625019798	24.225851919222087	33.73774326813001	20.44453418762811
20	22.899274211428907	23.467767114175956	33.834009800860635	19.79894887353451
21	21.636214315772325	24.470138144171294	34.775068891090996	19.11857864896539
22	19.1477640372668	25.06736394857942	33.989212718494485	21.795659295659295
23	21.073886543499803	25.473414147447297	35.385586490558865	18.06711281849403
24	20.837971321396736	25.128958692494603	32.26071375242646	21.7723562336822
25	20.450473074782465	26.155311514427538	33.40245066764404	19.99176474314596
26	20.149908823941974	27.056551780308684	33.18027972724105	19.613259668508288
27	18.390075158030957	27.206494540748682	32.85420654205184	21.54922375916851
28	19.555171696055673	26.373466635897575	32.8106547029199	21.260706965126854
29	19.743971746734175	26.28195121289044	31.671010538413853	22.30306650196153
30	20.176266170741307	26.5258980728594	34.18406477798743	19.11377097841186
31	21.635761829131994	25.95814046090289	33.65488165211922	18.751216057845895
32	20.681467504671925	26.257347251822388	31.99606562866231	21.065119614843372
33	20.061221442436914	26.16334315229343	32.99566517798562	20.77977022728404
34	18.76699652942747	27.373801476011423	33.92812702204967	19.931074972511436
35	18.195675585178346	30.38747562228225	31.91591893249352	19.500929860045883
36	21.5320292668359	27.162716458296572	31.50019683168854	19.80505744317899
37	20.412916683634915	28.452190261582526	31.207494536223816	19.92739851855874
38	20.21110764204687	26.20440631490355	32.84272469355342	20.74176134949616
39	19.663259442264966	25.72380894204099	33.81749403848852	20.795437577205533
40	17.789116338840095	26.6508975072511	33.196569246293	22.3634169076158
41	20.409918959642717	25.44897986886937	33.110540223799894	21.03056094768802
42	18.55749521495378	28.472665282057548	32.11144972194696	20.858389781041716
43	19.078646702956096	28.7209673259397	30.583685141696193	21.61670082940801
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	128.0
1	193.5
2	259.0
3	754.5
4	1250.0
5	1250.0
6	2278.5
7	3307.0
8	3809.0
9	4311.0
10	6767.5
11	9224.0
12	9224.0
13	17320.5
14	25417.0
15	42276.5
16	59136.0
17	57017.0
18	54898.0
19	54898.0
20	57703.5
21	60509.0
22	48222.5
23	35936.0
24	38651.0
25	41366.0
26	41366.0
27	43838.0
28	46310.0
29	47401.0
30	48492.0
31	51200.5
32	53909.0
33	53909.0
34	59053.0
35	64197.0
36	70059.0
37	75921.0
38	78483.5
39	81046.0
40	81046.0
41	83213.0
42	85380.0
43	89354.5
44	93329.0
45	104265.5
46	115202.0
47	115202.0
48	169397.0
49	223592.0
50	202528.5
51	181465.0
52	158169.5
53	134874.0
54	134874.0
55	112930.0
56	90986.0
57	71260.5
58	51535.0
59	44950.5
60	38366.0
61	38366.0
62	33266.5
63	28167.0
64	23981.5
65	19796.0
66	16887.0
67	13978.0
68	13978.0
69	11836.0
70	9694.0
71	8122.0
72	6550.0
73	5423.5
74	4297.0
75	4297.0
76	3389.0
77	2481.0
78	1890.5
79	1300.0
80	1012.0
81	724.0
82	724.0
83	531.5
84	339.0
85	261.5
86	184.0
87	138.5
88	93.0
89	93.0
90	66.5
91	40.0
92	29.0
93	18.0
94	9.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1768008.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.35791803210245
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.52093549787317	31.23388660028735
2	12.89641094579915	10.667374136093402
3	4.6144317356519835	5.725298684634809
4	2.1993288202359245	3.6383864429183177
5	1.1262667423238775	2.329002380565699
6	0.7474006973905886	1.8546562067889703
7	0.4735297410473494	1.3708942951199283
8	0.3375674573912215	1.11688697864731
9	0.2640212623498901	0.9827432754299074
>10	1.5540823138753304	12.253213881474172
>50	0.15597313926552958	4.478538442456645
>100	0.09175568810728087	7.152222857463999
>500	0.00839137954896454	2.3916864552697525
>1k	0.008528943148127893	7.121636425100891
>5k	9.629451941434718E-4	3.1742557305025287
>10k+	4.126907974900593E-4	4.509317207246428
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	28085	1.5885109117153315	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	27410	1.5503323514373237	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	23764	1.3441115651060402	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9527	0.538855027805304	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	8386	0.4743191207279605	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	8103	0.45831240582621796	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	7792	0.44072198768331367	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	7702	0.43563151297957925	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	7451	0.4214347446391645	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	6832	0.3864235908434804	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	4744	0.26832457771684287	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4359	0.24654865815086807	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4228	0.2391391894154325	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	4208	0.23800797281460262	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3656	0.20678639463169848	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3656	0.20678639463169848	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3584	0.20271401486871102	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	2975	0.16826846937344175	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	2963	0.16758973941294383	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	2956	0.16719381360265337	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2896	0.16380016380016382	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2587	0.14632286731734245	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	2509	0.14191112257410599	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	2476	0.14004461518273673	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	2415	0.13659440455020566	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG	2387	0.13501070130904386	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	2238	0.1265831376328614	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	2201	0.12449038692132613	No Hit
ATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGGA	2063	0.11668499237560011	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA	2053	0.11611938407518518	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCCT	1978	0.1118773218220732	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1975	0.11170763933194873	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG	1972	0.11153795684182424	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA	1953	0.11046330107103589	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	1910	0.10803118537925167	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT	1896	0.10723933375867077	No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT	1859	0.10514658304713555	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	1818	0.10282758901543432	No Hit
ACGCACATGCGAGGGTCCAAAAAAAAAAAAAAAAAAAAAAAAA	1792	0.10135700743435551	No Hit
GCCGAGGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAA	1788	0.10113076411418953	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	1774	0.10033891249360864	No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG	1769	0.10005610834340115	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	5.6560830041493025E-5	0.0	0.0	0.0	0.0
3	5.6560830041493025E-5	0.0	0.0	0.0	0.0
4	5.6560830041493025E-5	0.0	0.0	0.0	0.0
5	1.1312166008298605E-4	0.0	0.0	0.0	0.0
6	1.6968249012447908E-4	0.0	0.0	0.0	5.6560830041493025E-5
7	1.6968249012447908E-4	0.0	0.0	0.0	5.6560830041493025E-5
8	1.6968249012447908E-4	0.0	0.0	0.0	5.6560830041493025E-5
9	1.6968249012447908E-4	0.0	0.0	5.6560830041493025E-5	5.6560830041493025E-5
10	1.6968249012447908E-4	0.0	0.0	1.1312166008298605E-4	5.6560830041493025E-5
11	1.6968249012447908E-4	0.0	0.0	2.828041502074651E-4	1.1312166008298605E-4
12	1.6968249012447908E-4	0.0	0.0	6.787299604979163E-4	1.1312166008298605E-4
13	2.262433201659721E-4	0.0	0.0	8.484124506223954E-4	1.1312166008298605E-4
14	2.262433201659721E-4	0.0	0.0	0.0011312166008298605	1.1312166008298605E-4
15	2.262433201659721E-4	0.0	0.0	0.0014705815810788186	1.1312166008298605E-4
16	2.262433201659721E-4	0.0	0.0	0.002545237351867186	1.1312166008298605E-4
17	2.828041502074651E-4	0.0	0.0	0.0035067714625725676	1.1312166008298605E-4
18	2.828041502074651E-4	0.0	0.0	0.004015818932946005	1.1312166008298605E-4
19	2.828041502074651E-4	0.0	0.0	0.005203596363817359	1.1312166008298605E-4
20	2.828041502074651E-4	0.0	0.0	0.008144759525974996	1.1312166008298605E-4
21	2.828041502074651E-4	0.0	0.0	0.013913964190207285	1.1312166008298605E-4
22	2.828041502074651E-4	0.0	0.0	0.022963696996846167	1.1312166008298605E-4
23	2.828041502074651E-4	0.0	0.0	0.027997610870539046	1.6968249012447908E-4
24	2.828041502074651E-4	0.0	0.0	0.03467178881543522	1.6968249012447908E-4
25	2.828041502074651E-4	0.0	0.0	0.04049755430970901	1.6968249012447908E-4
26	3.3936498024895816E-4	0.0	0.0	0.05067850371717775	1.6968249012447908E-4
27	3.3936498024895816E-4	0.0	0.0	0.13416228885842146	1.6968249012447908E-4
28	3.3936498024895816E-4	0.0	0.0	0.2368201953837313	1.6968249012447908E-4
29	3.3936498024895816E-4	0.0	0.0	0.35706852005194545	1.6968249012447908E-4
30	3.9592581029045115E-4	0.0	0.0	0.5681535377667974	1.6968249012447908E-4
31	4.524866403319442E-4	0.0	0.0	0.8379486970647192	1.6968249012447908E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6845	0.0	29.945942	1
GTATTGG	745	0.0	21.107382	1
ATTGGAC	695	0.0	21.028776	3
GTCGTCA	45	0.0038266103	20.555555	24
CGTATAC	65	6.904817E-5	19.923077	3
TTGGACC	1245	0.0	19.614458	4
ACGTTTA	135	5.638867E-11	19.185186	26
ACCGTCC	60	9.239494E-4	18.5	8
GGACCCT	1275	0.0	17.84706	6
TGGACCC	1330	0.0	17.80451	5
ACGGGCG	105	4.800877E-7	17.619047	5
CTTAGAC	200	0.0	17.575	3
TAATACT	265	0.0	17.452831	4
GACCCTC	1305	0.0	17.153255	7
GTATCAA	12325	0.0	16.676268	2
GATACTG	650	0.0	16.507692	36
CTAATAC	180	1.0913936E-11	16.444445	3
TCACGTT	150	4.6748028E-9	16.033333	24
CGTTGAC	185	1.8189894E-11	16.0	32
CAGACGT	70	0.0025932342	15.857142	4
>>END_MODULE