FastQCFastQC Report
Wed 25 May 2016
SRR2088336_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088336_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3115948
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT407561.3079807493578197No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT400651.2858045127839104No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT353971.1359945673034337No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140930.45228611003777985No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA118230.3794350868499731No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG118120.37908206427064894No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG114670.3680099924645726No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA111620.3582216391287659No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG108290.34753468286377054No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA107030.3434909696824209No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA70680.2268330536966599No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA65750.21101122355058557No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA62020.19904054881532043No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC57470.18443825121600232No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT52450.1683275844141173No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA52270.16774991110249593No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA51030.16377038384465978No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG43850.14072763730331828No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG42560.13658764523669845No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG41650.1336671857168348No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA40310.12936672884143124No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC39970.128275568141702No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT38140.122402556140218No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC36850.11826256407359816No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35120.11271048168968159No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG35040.11245373799562766No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31590.1013816661895513No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA99200.029.9879021
GTATTGG11750.020.3106381
ATTGGAC12650.019.5968363
TAACGTG500.0070375218.4999985
GTATCAA176950.016.8324382
TTGGACC20700.016.6231884
GGACCCT20150.016.3424326
TGGACCC21000.016.0333355
GATACTG12000.015.57083236
TATACCG2750.015.4727275
TATTGGA16800.014.976192
TGATACT12550.014.88844635
ACGTTTA2950.014.42372926
TTTTTAC24000.014.1833321
GACCCTC23050.014.0455537
TAATACT7900.013.8164574
CTAATAC5550.013.6666673
TTGATAC36800.013.52309834
ATACTGT14600.013.30479437
GTATTAG4450.013.3033711
Produced by FastQC (version 0.11.5)