##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088336_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3115948
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.913321403309684	31.0	31.0	33.0	28.0	34.0
2	31.2315799236701	31.0	31.0	34.0	28.0	34.0
3	31.292800778446882	31.0	31.0	34.0	28.0	34.0
4	35.18400724273961	35.0	35.0	37.0	33.0	37.0
5	34.93989116634809	35.0	35.0	37.0	32.0	37.0
6	34.990147139811064	36.0	35.0	37.0	32.0	37.0
7	34.79129080459622	35.0	35.0	37.0	32.0	37.0
8	34.85673092105517	35.0	35.0	37.0	32.0	37.0
9	36.32750000962789	38.0	35.0	39.0	32.0	39.0
10	36.1256266150783	38.0	35.0	39.0	31.0	39.0
11	36.22475567628214	38.0	35.0	39.0	32.0	39.0
12	35.97987033159732	38.0	35.0	39.0	30.0	39.0
13	36.05538571247017	38.0	35.0	39.0	30.0	39.0
14	36.85228123190759	38.0	36.0	40.0	31.0	41.0
15	36.985259702665125	38.0	36.0	40.0	31.0	41.0
16	36.959257022260964	38.0	36.0	40.0	31.0	41.0
17	37.04425651519217	39.0	36.0	40.0	31.0	41.0
18	37.05537865201858	39.0	36.0	40.0	31.0	41.0
19	37.14291348892857	39.0	36.0	40.0	31.0	41.0
20	37.12660288297494	39.0	36.0	40.0	31.0	41.0
21	37.05665948212229	39.0	36.0	40.0	31.0	41.0
22	36.97426946791153	39.0	36.0	40.0	31.0	41.0
23	36.849096005453234	39.0	35.0	40.0	31.0	41.0
24	36.7621266465294	39.0	35.0	40.0	30.0	41.0
25	36.617094380265655	38.0	35.0	40.0	30.0	41.0
26	36.364743249887354	38.0	35.0	40.0	30.0	41.0
27	36.24550152955056	38.0	35.0	40.0	30.0	41.0
28	36.071441179377835	38.0	34.0	40.0	29.0	41.0
29	35.84771408251999	38.0	34.0	40.0	29.0	41.0
30	35.61537836960052	38.0	34.0	40.0	27.0	41.0
31	35.35758074268249	38.0	34.0	40.0	27.0	41.0
32	35.24480061926579	37.0	34.0	40.0	27.0	41.0
33	35.05238790891247	37.0	33.0	40.0	26.0	41.0
34	35.18965079006453	38.0	33.0	40.0	27.0	41.0
35	35.09605198803061	38.0	33.0	40.0	26.0	41.0
36	35.02757234716369	38.0	33.0	40.0	25.0	41.0
37	34.90961883831181	38.0	33.0	40.0	25.0	41.0
38	34.85773671447662	38.0	33.0	40.0	25.0	41.0
39	34.737530921568656	38.0	33.0	40.0	24.0	41.0
40	34.53147324666522	38.0	33.0	40.0	23.0	41.0
41	34.391107938900134	38.0	33.0	40.0	23.0	41.0
42	34.21983261594866	38.0	33.0	40.0	22.0	41.0
43	33.83697641937542	37.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	0.0
14	4.0
15	17.0
16	50.0
17	117.0
18	270.0
19	714.0
20	1513.0
21	2839.0
22	5252.0
23	9088.0
24	14590.0
25	22808.0
26	34053.0
27	48957.0
28	67205.0
29	88293.0
30	112426.0
31	135588.0
32	162722.0
33	195078.0
34	230481.0
35	273613.0
36	333911.0
37	421861.0
38	498877.0
39	455619.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.74310611088504	22.178354709385395	14.299468412181463	22.77907076754811
2	18.520784043892903	23.724401049054734	35.84427596352699	21.910538943525374
3	19.6068740556646	25.26357949490813	33.199206148497986	21.93034030092928
4	13.247685776527721	16.290547852531557	36.59650289414329	33.86526347679743
5	12.043108549950128	38.558698668912314	35.593950861824396	13.80424191931316
6	31.52411400960478	37.395264619306865	13.52824244820517	17.55237892288318
7	24.629262105786104	34.054997066703294	21.988492747632502	19.3272480798781
8	26.281311498137967	34.47711579268974	19.49265520477235	19.748917504399945
9	23.906175584444927	14.350688779145223	23.105359909728918	38.637775726680935
10	15.9854400651102	27.390829371992087	34.380162955222616	22.243567607675097
11	32.09838546727995	22.34754880376694	24.733500045572004	20.82056568338111
12	21.26396204301227	27.15924655995543	31.431557907898334	20.145233489133965
13	31.589936674167863	21.32368704484157	27.5410565259754	19.545319755015168
14	20.174502270256113	23.218583878806704	28.69184594864869	27.915067902288488
15	21.596766056429697	29.602162808878713	27.089925762560863	21.711145372130726
16	21.180648714291767	28.667166461057757	27.751169146596798	22.401015678053675
17	21.011615084719	26.55342772087339	27.546351864665265	24.888605329742344
18	22.896595193501305	25.176735940394384	31.10555760237334	20.821111263730973
19	22.077165600966385	24.27771580270274	32.41209416845211	21.23302442787877
20	23.302571159724103	23.761372140998503	32.50381585315288	20.432240846124518
21	22.0494051890468	24.732794000413357	33.06033990297656	20.157460907563284
22	20.231242626642036	25.07538636716659	32.46248011841019	22.230890887781182
23	21.701292832871406	25.474398160688178	33.726846532740595	19.097462473699817
24	21.59583536053875	25.25080649612895	30.86126597748101	22.29209216585129
25	21.2855285133128	25.886535975568272	32.016676786647274	20.811258724471653
26	21.020248091431565	26.774708692186135	31.79536372237277	20.409679494009527
27	19.52831048528409	26.85612853616299	31.52369680110194	22.091864177450972
28	20.434294795676948	26.074119337036432	31.679315572660393	21.812270294626227
29	20.66712923322212	26.079510954611568	30.601794381677745	22.65156543048857
30	20.967551448226992	26.19440375770071	32.68206016274983	20.155984631322475
31	22.073507003326114	25.864969505267737	32.335231525044705	19.726291966361444
32	21.285303862580506	26.01904781466186	30.927088642044094	21.768559680713544
33	20.891555314787023	25.9378526214173	31.808553929654792	21.362038134140878
34	19.77257001721466	26.74470177294358	32.721983807175214	20.76074440266654
35	19.393552138867527	29.256232774102774	30.891754291149915	20.458460795879777
36	22.116768315774205	26.49575666859652	30.65041521873921	20.737059796890065
37	21.03237923097561	27.64038424261252	30.468351846693203	20.85888467971866
38	20.927788268610385	25.675524752017687	31.916065351539885	21.480621627832043
39	20.377105137826433	25.402445740429556	32.736874941430344	21.483574180313664
40	18.809749071550616	26.096680689151423	32.27277862146609	22.820791617831876
41	20.994830465720224	24.973555399512442	32.171332769353015	21.860281365414313
42	19.290469545704873	27.572924836999846	31.42337420265037	21.713231414644916
43	19.859959152078275	27.673632550992505	30.14142084527726	22.324987451651953
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	298.0
1	405.5
2	513.0
3	1345.0
4	2177.0
5	2177.0
6	3782.5
7	5388.0
8	6136.5
9	6885.0
10	10732.5
11	14580.0
12	14580.0
13	26125.0
14	37670.0
15	62003.0
16	86336.0
17	83838.5
18	81341.0
19	81341.0
20	85098.5
21	88856.0
22	71754.5
23	54653.0
24	59616.0
25	64579.0
26	64579.0
27	69797.5
28	75016.0
29	78116.5
30	81217.0
31	88686.5
32	96156.0
33	96156.0
34	105785.0
35	115414.0
36	125946.5
37	136479.0
38	144306.0
39	152133.0
40	152133.0
41	156752.5
42	161372.0
43	169356.0
44	177340.0
45	191805.5
46	206271.0
47	206271.0
48	284701.5
49	363132.0
50	331014.0
51	298896.0
52	265591.0
53	232286.0
54	232286.0
55	200189.5
56	168093.0
57	137925.0
58	107757.0
59	95364.0
60	82971.0
61	82971.0
62	73970.0
63	64969.0
64	57065.5
65	49162.0
66	42402.5
67	35643.0
68	35643.0
69	30590.0
70	25537.0
71	21488.5
72	17440.0
73	14364.5
74	11289.0
75	11289.0
76	9023.0
77	6757.0
78	5114.0
79	3471.0
80	2765.0
81	2059.0
82	2059.0
83	1504.5
84	950.0
85	702.5
86	455.0
87	359.5
88	264.0
89	264.0
90	183.5
91	103.0
92	63.5
93	24.0
94	16.0
95	8.0
96	8.0
97	6.0
98	4.0
99	4.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3115948.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.520918078475404
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.00619303835619	28.85271774290236
2	13.716982434642697	10.842154781668002
3	5.297369404298596	6.280707067761206
4	2.529271577999816	3.9983653932938776
5	1.3882485517027756	2.7432428642203757
6	0.858219349067409	2.0350569952713338
7	0.5426218257714255	1.501143890673368
8	0.4029740178498677	1.2740722517758964
9	0.28537626999650567	1.01504989692655
>10	1.6739873776237881	12.602731026242022
>50	0.16604176835333403	4.581253557124407
>100	0.1152763481416757	8.582591021065596
>500	0.009251164742467751	2.4011491193766767
>1k	0.0067951033063997765	5.547990091197457
>5k	5.730810017445594E-4	1.331969301423131
>10k+	8.186871453493707E-4	6.409804999077713
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	40756	1.3079807493578197	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	40065	1.2858045127839104	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	35397	1.1359945673034337	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14093	0.45228611003777985	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	11823	0.3794350868499731	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	11812	0.37908206427064894	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	11467	0.3680099924645726	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	11162	0.3582216391287659	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	10829	0.34753468286377054	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	10703	0.3434909696824209	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	7068	0.2268330536966599	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	6575	0.21101122355058557	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	6202	0.19904054881532043	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	5747	0.18443825121600232	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5245	0.1683275844141173	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	5227	0.16774991110249593	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	5103	0.16377038384465978	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	4385	0.14072763730331828	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	4256	0.13658764523669845	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	4165	0.1336671857168348	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4031	0.12936672884143124	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3997	0.128275568141702	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	3814	0.122402556140218	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	3685	0.11826256407359816	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3512	0.11271048168968159	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG	3504	0.11245373799562766	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3159	0.1013816661895513	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	6.41859235134861E-5	0.0
5	3.209296175674305E-5	0.0	0.0	6.41859235134861E-5	0.0
6	6.41859235134861E-5	0.0	0.0	6.41859235134861E-5	0.0
7	6.41859235134861E-5	0.0	0.0	9.627888527022915E-5	0.0
8	1.283718470269722E-4	0.0	0.0	1.283718470269722E-4	0.0
9	1.283718470269722E-4	0.0	0.0	1.283718470269722E-4	0.0
10	1.283718470269722E-4	0.0	0.0	1.6046480878371527E-4	0.0
11	1.283718470269722E-4	0.0	0.0	3.2092961756743053E-4	0.0
12	1.283718470269722E-4	0.0	0.0	5.455803498646319E-4	0.0
13	1.283718470269722E-4	0.0	0.0	6.739521968916041E-4	0.0
14	1.283718470269722E-4	0.0	0.0	7.702310821618332E-4	0.0
15	1.283718470269722E-4	0.0	0.0	0.0011232536614860069	0.0
16	1.283718470269722E-4	0.0	0.0	0.0016367410495938958	0.0
17	1.283718470269722E-4	0.0	0.0	0.002503251017025958	0.0
18	1.283718470269722E-4	0.0	0.0	0.0032734820991877915	0.0
19	1.283718470269722E-4	0.0	0.0	0.00420417799013334	0.0
20	1.6046480878371527E-4	0.0	0.0	0.006418592351348611	0.0
21	1.6046480878371527E-4	0.0	0.0	0.012355790276346075	0.0
22	1.6046480878371527E-4	0.0	0.0	0.01986554332742395	0.0
23	1.925577705404583E-4	0.0	0.0	0.02471158055269215	0.0
24	1.925577705404583E-4	0.0	0.0	0.032413891374310486	0.0
25	1.925577705404583E-4	0.0	0.0	0.037677137102416346	0.0
26	1.925577705404583E-4	0.0	0.0	0.048107349673357835	0.0
27	1.925577705404583E-4	0.0	0.0	0.1264141763598109	0.0
28	1.925577705404583E-4	0.0	0.0	0.22140934315977032	0.0
29	1.925577705404583E-4	0.0	0.0	0.33331750080553335	0.0
30	1.925577705404583E-4	0.0	0.0	0.52096504819721	0.0
31	1.925577705404583E-4	0.0	0.0	0.7859566334226373	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9920	0.0	29.987902	1
GTATTGG	1175	0.0	20.310638	1
ATTGGAC	1265	0.0	19.596836	3
TAACGTG	50	0.00703752	18.499998	5
GTATCAA	17695	0.0	16.832438	2
TTGGACC	2070	0.0	16.623188	4
GGACCCT	2015	0.0	16.342432	6
TGGACCC	2100	0.0	16.033335	5
GATACTG	1200	0.0	15.570832	36
TATACCG	275	0.0	15.472727	5
TATTGGA	1680	0.0	14.97619	2
TGATACT	1255	0.0	14.888446	35
ACGTTTA	295	0.0	14.423729	26
TTTTTAC	2400	0.0	14.183332	1
GACCCTC	2305	0.0	14.045553	7
TAATACT	790	0.0	13.816457	4
CTAATAC	555	0.0	13.666667	3
TTGATAC	3680	0.0	13.523098	34
ATACTGT	1460	0.0	13.304794	37
GTATTAG	445	0.0	13.303371	1
>>END_MODULE