FastQCFastQC Report
Wed 25 May 2016
SRR2088335_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088335_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3699611
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT450701.218236187534311No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT438131.1842596424326775No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT388051.0488940594024614No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA161130.4355322762312037No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153020.4136110526214783No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG145590.39352786009123664No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA128790.3481176804804613No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG122660.3315483708962915No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA119450.32287178300637553No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG115540.31230310429934394No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA89440.24175514668974657No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA72900.19704774366818564No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA71350.19285811400171532No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC66990.1810730911979665No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA61930.167395977577102No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA59430.1606385103731176No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG54970.1485831888812094No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG54530.14739387465330814No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG54390.147015456489885No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT53480.1445557384276347No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC49260.133149133787309No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC48060.12990554952939645No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT43890.11863409423315047No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA43780.11833676567617514No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC37210.1005781418641041No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37170.10047002238884034No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA109350.030.232741
GTATTGG13250.023.1773591
ATTGGAC15050.020.4053173
ACGTTTA1650.019.06060626
TTGGACC22300.018.7488784
CGAATTA2350.018.10638215
TATACCG2500.017.765
TGGACCC23400.017.5512835
GTATCAA189550.017.4605662
GGACCCT22500.017.431116
TACCCCG3500.016.9142865
GATACCT13750.016.0109136
TATTGGA19000.015.9684212
GACCCTC24400.015.4672137
TTGATAC35950.015.28372834
ACCCCGC3350.014.9104486
GTATTAG6650.014.744361
GATACTG11050.014.7330336
TCACGTT2450.014.34693824
TGATACC27100.014.19926335
Produced by FastQC (version 0.11.5)