##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088335_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3699611
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.938510562326687	31.0	31.0	33.0	28.0	34.0
2	31.25348205527554	31.0	31.0	34.0	28.0	34.0
3	31.321728149256774	31.0	31.0	34.0	28.0	34.0
4	35.206718219834464	35.0	35.0	37.0	33.0	37.0
5	34.96338209611767	35.0	35.0	37.0	32.0	37.0
6	35.00686045100417	36.0	35.0	37.0	32.0	37.0
7	34.81834603692118	35.0	35.0	37.0	32.0	37.0
8	34.879182703262586	35.0	35.0	37.0	32.0	37.0
9	36.354016138453474	38.0	35.0	39.0	32.0	39.0
10	36.15815311393549	38.0	35.0	39.0	31.0	39.0
11	36.25358557967311	38.0	35.0	39.0	32.0	39.0
12	36.00315898076852	38.0	35.0	39.0	30.0	39.0
13	36.08930209149016	38.0	35.0	39.0	31.0	39.0
14	36.90275193797402	39.0	36.0	40.0	31.0	41.0
15	37.03363353606636	39.0	36.0	40.0	31.0	41.0
16	37.00258675844569	38.0	36.0	40.0	31.0	41.0
17	37.07528656391172	39.0	36.0	40.0	31.0	41.0
18	37.082382174774594	39.0	36.0	40.0	31.0	41.0
19	37.166924576664954	39.0	36.0	40.0	31.0	41.0
20	37.14357022941061	39.0	36.0	40.0	31.0	41.0
21	37.07241572154478	39.0	36.0	40.0	31.0	41.0
22	36.99181562602122	39.0	36.0	40.0	31.0	41.0
23	36.87510389605826	39.0	35.0	40.0	31.0	41.0
24	36.78963680235571	39.0	35.0	40.0	30.0	41.0
25	36.66204690168777	38.0	35.0	40.0	30.0	41.0
26	36.415030931630376	38.0	35.0	40.0	30.0	41.0
27	36.28759104673437	38.0	35.0	40.0	30.0	41.0
28	36.11894412682847	38.0	35.0	40.0	30.0	41.0
29	35.90073199587741	38.0	34.0	40.0	29.0	41.0
30	35.68086996173381	38.0	34.0	40.0	28.0	41.0
31	35.44260031662788	38.0	34.0	40.0	27.0	41.0
32	35.33428352332178	38.0	34.0	40.0	27.0	41.0
33	35.15450975791779	38.0	33.0	40.0	27.0	41.0
34	35.31180305172625	38.0	34.0	40.0	27.0	41.0
35	35.22194955091224	38.0	34.0	40.0	26.0	41.0
36	35.170153294495016	38.0	33.0	40.0	26.0	41.0
37	35.05484928009999	38.0	33.0	40.0	25.0	41.0
38	35.01202748072703	38.0	33.0	40.0	25.0	41.0
39	34.91054086497202	38.0	33.0	40.0	25.0	41.0
40	34.71053091798029	38.0	33.0	40.0	24.0	41.0
41	34.590002300241835	38.0	33.0	40.0	24.0	41.0
42	34.41752876180766	38.0	33.0	40.0	23.0	41.0
43	34.0524679486573	37.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	0.0
13	3.0
14	3.0
15	14.0
16	46.0
17	118.0
18	292.0
19	734.0
20	1474.0
21	3060.0
22	5606.0
23	10050.0
24	16337.0
25	25875.0
26	38606.0
27	55252.0
28	76971.0
29	102013.0
30	129826.0
31	157798.0
32	191187.0
33	229409.0
34	274699.0
35	325346.0
36	398445.0
37	501355.0
38	601925.0
39	553165.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.7232003580917	22.100485699712756	14.042124969354886	23.134188972840658
2	18.39360949029506	23.501227561492275	35.8567698063391	22.248393141873564
3	19.58838375169714	25.06950055019298	33.34034307931294	22.00177261879695
4	13.183277917597284	16.16153698321256	36.831791234267605	33.82339386492256
5	12.211040566156822	38.35851931459821	35.77073373389797	13.659706385347
6	31.916896127728023	37.12968741848805	13.617350580912428	17.336065872871497
7	25.094800507404695	33.799094012857026	21.869974978450436	19.23613050128784
8	26.50140785071728	34.179647535916615	19.49369812123491	19.8252464921312
9	23.85164278082209	14.443059013501689	23.02585325862638	38.67944494704984
10	15.93551322017369	27.195615971517007	34.667942116076524	22.200928692232775
11	32.20406145402854	22.291586872241435	24.72249109433397	20.78186057939605
12	21.29923929840191	26.82344170779036	31.380326201862847	20.496992791944884
13	31.58980768518636	20.862328498861096	27.52189351799419	20.025970297958352
14	20.390846497104693	22.881702968230986	28.436016651480383	28.291433883183934
15	21.848080784709527	29.464638309270896	26.62052848258912	22.066752423430465
16	21.57964715749845	28.574598788899696	27.444588093180606	22.401165960421242
17	21.165441447763023	26.47272915990357	27.389041712763856	24.972787679569556
18	22.982254080226273	25.119505807502463	30.87846262755733	21.019777484713934
19	22.141084562674294	24.159431897029172	32.00125634830256	21.69822719199397
20	23.516337258160387	23.654351768334564	32.111646332546854	20.717664640958198
21	22.55650661650644	24.59007176700469	32.4926323335075	20.360789282981372
22	20.52402266076082	25.083123604076214	32.00941936868497	22.38343436647799
23	22.114081723727168	25.32339210798108	33.16751409810383	19.39501207018792
24	21.854459833750088	25.072284626681025	30.387356941040554	22.685898598528333
25	21.76482878875644	25.671239489773384	31.506339450282745	21.05759227118743
26	21.496908729052866	26.409100848710853	31.354377527799542	20.73961289443674
27	19.896740495149352	26.701347790348766	31.148220718340387	22.253690996161488
28	20.938795997741384	25.89218163747486	31.17919694800345	21.989825416780302
29	21.09040653192998	25.899209403367003	30.14789933320017	22.862484731502853
30	21.23331344835984	26.25192216154617	32.14924488006982	20.365519510024164
31	22.459388297850776	25.751923648188956	31.839185254882203	19.949502799078065
32	21.70168701520241	25.755113172709237	30.376058455875498	22.167141356212856
33	21.30351001767483	25.954350335751514	31.225661292498046	21.516478354075605
34	20.378818205481604	26.889070229275458	31.849024127131205	20.883087438111737
35	19.851519524620294	29.57432551692597	30.188714435112228	20.385440523341508
36	22.52682782054654	26.783815920106196	29.93314702545754	20.756209233889724
37	21.57534940835672	27.78492117144208	29.85430089812145	20.785428522079755
38	21.44503841079508	26.030060998304954	31.13032694518424	21.394573645715724
39	21.043671888747223	25.58563589523331	31.860917269410216	21.50977494660925
40	19.356386387649945	26.602526589957705	31.33956515968841	22.701521862703945
41	21.66281806384509	25.416537035920804	31.232689058390196	21.687955841843912
42	19.72885797993357	28.060436624282932	30.610515537984938	21.60018985779856
43	20.286808532032154	28.11163119582032	29.309919340168467	22.291640931979064
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	210.0
1	321.0
2	432.0
3	1357.0
4	2282.0
5	2282.0
6	3777.0
7	5272.0
8	5934.0
9	6596.0
10	10196.0
11	13796.0
12	13796.0
13	26484.0
14	39172.0
15	65408.5
16	91645.0
17	89414.0
18	87183.0
19	87183.0
20	92792.5
21	98402.0
22	80577.5
23	62753.0
24	69654.5
25	76556.0
26	76556.0
27	83234.0
28	89912.0
29	95045.0
30	100178.0
31	108222.5
32	116267.0
33	116267.0
34	128140.0
35	140013.0
36	153048.5
37	166084.0
38	173390.5
39	180697.0
40	180697.0
41	187476.5
42	194256.0
43	201473.5
44	208691.0
45	223413.5
46	238136.0
47	238136.0
48	326139.0
49	414142.0
50	385354.5
51	356567.0
52	321349.5
53	286132.0
54	286132.0
55	247805.5
56	209479.0
57	173557.0
58	137635.0
59	122590.5
60	107546.0
61	107546.0
62	96661.5
63	85777.0
64	74152.5
65	62528.0
66	52853.5
67	43179.0
68	43179.0
69	36480.5
70	29782.0
71	25046.5
72	20311.0
73	16526.5
74	12742.0
75	12742.0
76	10024.5
77	7307.0
78	5547.5
79	3788.0
80	2898.0
81	2008.0
82	2008.0
83	1565.0
84	1122.0
85	821.5
86	521.0
87	405.5
88	290.0
89	290.0
90	227.5
91	165.0
92	99.0
93	33.0
94	26.0
95	19.0
96	19.0
97	10.5
98	2.0
99	2.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3699611.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.01473704654053
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.05185640191972	27.010176377875357
2	14.23063931529117	10.819480231499108
3	5.724774583479826	6.528774013251125
4	2.8291959771275628	4.302045644945384
5	1.6565162745978084	3.14860152960753
6	1.0016291128706616	2.284600040636272
7	0.6520854219884322	1.7352199094140868
8	0.45016829763283317	1.3690423568960757
9	0.33332085485161617	1.140399418438106
>10	1.7560547169226757	12.563566329923331
>50	0.17081986888136444	4.53011857203262
>100	0.12525804966941437	9.05520734661155
>500	0.00995010665079618	2.5116934168043086
>1k	0.006299348094630525	5.211641384063515
>5k	7.158350107534687E-4	1.7399803764575925
>10k+	7.158350107534687E-4	6.049453051544104
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	45070	1.218236187534311	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	43813	1.1842596424326775	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	38805	1.0488940594024614	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	16113	0.4355322762312037	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15302	0.4136110526214783	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	14559	0.39352786009123664	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	12879	0.3481176804804613	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	12266	0.3315483708962915	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	11945	0.32287178300637553	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	11554	0.31230310429934394	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	8944	0.24175514668974657	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	7290	0.19704774366818564	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	7135	0.19285811400171532	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	6699	0.1810730911979665	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	6193	0.167395977577102	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	5943	0.1606385103731176	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	5497	0.1485831888812094	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	5453	0.14739387465330814	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	5439	0.147015456489885	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5348	0.1445557384276347	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	4926	0.133149133787309	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	4806	0.12990554952939645	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	4389	0.11863409423315047	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4378	0.11833676567617514	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	3721	0.1005781418641041	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3717	0.10047002238884034	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.7029868815937676E-5	0.0
3	0.0	0.0	0.0	2.7029868815937676E-5	0.0
4	0.0	0.0	0.0	2.7029868815937676E-5	0.0
5	0.0	0.0	0.0	2.7029868815937676E-5	0.0
6	0.0	0.0	0.0	2.7029868815937676E-5	0.0
7	5.405973763187535E-5	0.0	0.0	2.7029868815937676E-5	0.0
8	1.081194752637507E-4	0.0	0.0	2.7029868815937676E-5	0.0
9	1.081194752637507E-4	0.0	0.0	5.405973763187535E-5	0.0
10	1.3514934407968837E-4	0.0	0.0	8.108960644781302E-5	0.0
11	1.3514934407968837E-4	0.0	0.0	1.6217921289562605E-4	0.0
12	1.6217921289562605E-4	0.0	0.0	1.8920908171156373E-4	0.0
13	1.6217921289562605E-4	0.0	0.0	2.7029868815937674E-4	0.0
14	1.8920908171156373E-4	0.0	0.0	3.243584257912521E-4	0.0
15	1.8920908171156373E-4	0.0	0.0	5.946571139506288E-4	0.0
16	1.8920908171156373E-4	0.0	0.0	0.0010271350150056317	0.0
17	2.162389505275014E-4	0.0	0.0	0.0014596129160606346	0.0
18	2.162389505275014E-4	0.0	0.0	0.0018110012106678243	0.0
19	2.162389505275014E-4	0.0	0.0	0.0025948674063300168	0.0
20	2.162389505275014E-4	0.0	0.0	0.0038923011094950254	0.0
21	2.162389505275014E-4	0.0	0.0	0.006649347728720668	0.0
22	3.243584257912521E-4	0.0	0.0	0.010866007264006945	0.0
23	3.243584257912521E-4	0.0	0.0	0.013974442177839778	0.0
24	3.513882946071898E-4	0.0	0.0	0.01789377315615074	0.0
25	3.513882946071898E-4	0.0	0.0	0.021137357414063262	0.0
26	3.513882946071898E-4	0.0	0.0	0.027651555798704243	0.0
27	3.513882946071898E-4	0.0	0.0	0.07246707829552891	0.0
28	3.513882946071898E-4	0.0	0.0	0.13468983630981743	0.0
29	3.513882946071898E-4	0.0	0.0	0.21104921571484136	0.0
30	4.324779010550028E-4	0.0	0.0	0.3430090352742491	0.0
31	4.324779010550028E-4	0.0	0.0	0.5107564011459583	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10935	0.0	30.23274	1
GTATTGG	1325	0.0	23.177359	1
ATTGGAC	1505	0.0	20.405317	3
ACGTTTA	165	0.0	19.060606	26
TTGGACC	2230	0.0	18.748878	4
CGAATTA	235	0.0	18.106382	15
TATACCG	250	0.0	17.76	5
TGGACCC	2340	0.0	17.551283	5
GTATCAA	18955	0.0	17.460566	2
GGACCCT	2250	0.0	17.43111	6
TACCCCG	350	0.0	16.914286	5
GATACCT	1375	0.0	16.01091	36
TATTGGA	1900	0.0	15.968421	2
GACCCTC	2440	0.0	15.467213	7
TTGATAC	3595	0.0	15.283728	34
ACCCCGC	335	0.0	14.910448	6
GTATTAG	665	0.0	14.74436	1
GATACTG	1105	0.0	14.73303	36
TCACGTT	245	0.0	14.346938	24
TGATACC	2710	0.0	14.199263	35
>>END_MODULE