FastQCFastQC Report
Wed 25 May 2016
SRR2088334_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088334_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3090348
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT337381.0917217090114122No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT313131.0132515820224777No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT283180.9163369303392369No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111770.3616744780846688No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA109190.35332590374935124No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG103020.333360514738146No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA92190.2983159178189641No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA91380.2956948537834574No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG91380.2956948537834574No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG86160.2788035522213032No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA59810.1935380740292032No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA51370.16622723395552863No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA49930.1615675645590723No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC46810.15147161420008362No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA44430.1437702161698294No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT41140.13312416595153684No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG39760.12865864944659955No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA39600.12814090840254885No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG37410.12105432786210485No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG36900.11940402828419323No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC35140.11370887679963551No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC33330.10785193123881195No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT30950.10015053320855773No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA84500.029.906511
GTATTGG11650.022.072961
ATTGGAC12850.019.2918283
GTCGCTA500.007037507418.49999816
TTGGACC18750.017.6613354
GTATCAA146050.017.3536452
TGGACCC18700.017.1149735
GGACCCT18350.016.937336
ACGTTTA2100.016.73809426
TAATACT9200.016.2880424
CTAATAC7450.015.8926173
TATTGGA15650.015.8402562
CGACGAG2350.015.74468124
ATACTGG7650.015.4771246
GACCCTC20300.015.3103457
AATGCGT1853.0559022E-1015.035
GCGACGA2350.014.95744723
TACTGGT6600.014.8560617
CAATGCG2251.8189894E-1214.819
AATACTG8550.014.713455
Produced by FastQC (version 0.11.5)