##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088334_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3090348
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.945027226707154	31.0	31.0	33.0	28.0	34.0
2	31.26899850761144	31.0	31.0	34.0	28.0	34.0
3	31.340545142488807	31.0	31.0	34.0	28.0	34.0
4	35.22322178602539	35.0	35.0	37.0	33.0	37.0
5	34.970652172506135	35.0	35.0	37.0	32.0	37.0
6	35.0172999933988	36.0	35.0	37.0	32.0	37.0
7	34.832568047352595	35.0	35.0	37.0	32.0	37.0
8	34.88900052680151	36.0	35.0	37.0	32.0	37.0
9	36.37903174658647	38.0	35.0	39.0	32.0	39.0
10	36.173423187291526	38.0	35.0	39.0	32.0	39.0
11	36.275069021352934	38.0	35.0	39.0	32.0	39.0
12	36.03696832848598	38.0	35.0	39.0	30.0	39.0
13	36.10920032307041	38.0	35.0	39.0	31.0	39.0
14	36.94172468602242	39.0	36.0	40.0	31.0	41.0
15	37.067628953114664	39.0	36.0	40.0	31.0	41.0
16	37.03919332062279	39.0	36.0	40.0	31.0	41.0
17	37.0955099555131	39.0	36.0	40.0	31.0	41.0
18	37.09599630850636	39.0	36.0	40.0	31.0	41.0
19	37.18209405542677	39.0	36.0	40.0	31.0	41.0
20	37.158391546841976	39.0	36.0	40.0	31.0	41.0
21	37.07725278835911	39.0	36.0	40.0	31.0	41.0
22	37.00232886393377	39.0	36.0	40.0	31.0	41.0
23	36.88033515966487	39.0	35.0	40.0	31.0	41.0
24	36.80677418853799	39.0	35.0	40.0	30.0	41.0
25	36.66918677119858	38.0	35.0	40.0	30.0	41.0
26	36.43478889756105	38.0	35.0	40.0	30.0	41.0
27	36.31440828023252	38.0	35.0	40.0	30.0	41.0
28	36.15105062601364	38.0	35.0	40.0	30.0	41.0
29	35.94153829924656	38.0	34.0	40.0	29.0	41.0
30	35.734441234449974	38.0	34.0	40.0	28.0	41.0
31	35.51903054283854	38.0	34.0	40.0	27.0	41.0
32	35.40115029116462	38.0	34.0	40.0	27.0	41.0
33	35.22051432395316	38.0	33.0	40.0	27.0	41.0
34	35.395194651217274	38.0	34.0	40.0	27.0	41.0
35	35.32196794665196	38.0	34.0	40.0	27.0	41.0
36	35.28354573659666	38.0	34.0	40.0	26.0	41.0
37	35.18264091940455	38.0	34.0	40.0	26.0	41.0
38	35.16163843036448	38.0	34.0	40.0	26.0	41.0
39	35.07909335777071	38.0	33.0	40.0	25.0	41.0
40	34.90128425665977	38.0	33.0	40.0	24.0	41.0
41	34.79563304844633	38.0	33.0	40.0	24.0	41.0
42	34.647891758468624	38.0	33.0	40.0	24.0	41.0
43	34.284475405358876	38.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	0.0
14	2.0
15	11.0
16	27.0
17	90.0
18	228.0
19	541.0
20	1206.0
21	2342.0
22	4484.0
23	7865.0
24	13066.0
25	20503.0
26	31325.0
27	44403.0
28	62128.0
29	83013.0
30	106046.0
31	129700.0
32	157562.0
33	190296.0
34	228340.0
35	272254.0
36	332648.0
37	420091.0
38	512610.0
39	469566.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.73758036311768	22.160384526273415	13.772753100945264	23.329282009663636
2	18.54674619169103	23.275889964495907	35.71516864767334	22.462195196139724
3	19.62613919209099	25.183798070637998	32.71502109147578	22.47504164579523
4	13.39907350240167	16.28276168250307	36.77844695807722	33.53971785701804
5	12.353139516973492	38.27025305887881	35.762347800312455	13.614259623835245
6	32.09447609136576	37.227652031421705	13.823135776294448	16.854736100918082
7	25.318928483135235	33.50535279521918	22.107186633997205	19.068532087648382
8	26.21866534124959	34.57529702156521	19.573103093891046	19.63293454329415
9	24.1131419503564	14.437759113213138	22.653597588362217	38.79550134806824
10	15.978621177938537	27.151990649596748	34.72731873562459	22.142069436840124
11	32.56231984229608	22.381039287484775	24.433429503732267	20.62321136648688
12	21.11671565791296	26.74236040730688	31.34307204237193	20.797851892408232
13	31.059997126537205	21.15111307852708	27.2470284899953	20.541861304940415
14	20.38149101654571	22.78115603809021	28.136151656706627	28.701201288657458
15	22.071883166556	29.391641329714325	26.36851901468702	22.16795648904266
16	21.743149962399055	28.636321864074855	27.149045997408706	22.471482176117384
17	21.28278109779222	26.737506585018906	27.32187442967588	24.657837887512994
18	22.873961120236295	25.467326009886264	30.342666910004958	21.316045959872483
19	22.375214700739203	24.52416362170215	31.424098515765863	21.676523161792783
20	23.436875070380424	24.166533995524127	31.53690134573841	20.85968958835704
21	22.382883739954206	25.112382165374253	31.869679401802	20.63505469286954
22	20.84017722275938	25.437944205636388	31.35116174618522	22.370716825419017
23	22.076316324245685	25.700989014829396	32.46262233250106	19.760072328423853
24	21.991600945912886	25.457844877017088	29.869451595742614	22.681102581327412
25	21.84139132550768	26.053635383458435	30.90525727199655	21.199716019037336
26	21.592001936351505	26.747343664855865	30.72420970065507	20.936444698137556
27	20.357027752214314	26.89043434590538	30.412108927538256	22.340428974342046
28	21.10173352645074	26.15122957026199	30.685314404720764	22.061722498566503
29	21.335105302056597	26.12670158830009	29.81311489838685	22.725078211256466
30	21.525310418114724	26.298235667957137	31.447817527346434	20.728636386581705
31	22.452649345640037	26.011180617846275	31.18153683662811	20.35463319988558
32	21.706131477749434	26.054929736068562	30.071014655954603	22.167924130227405
33	21.386394024232867	26.060204222954823	30.81711185924692	21.736289893565385
34	20.603278336290927	26.812934983374042	31.446361380660043	21.137425299674987
35	20.217625976103662	28.985538198287053	29.91731028350205	20.87952554210723
36	22.597552120343728	26.584158159534137	29.62074174170676	21.197547978415376
37	21.638372118609293	27.294757742493726	29.711055195078355	21.355814943818626
38	21.49566974334282	25.820101813776315	30.87251015096035	21.81171829192052
39	21.048212046022	25.505833000037537	31.649930687417726	21.796024266522736
40	19.71438168128638	26.386672310044045	31.07290829382322	22.826037714846354
41	21.563623255374477	25.193635150474964	31.177103678938423	22.06563791521214
42	19.869283329903297	27.555666869880024	30.578206726232775	21.9968430739839
43	20.48869577147946	27.433026960070517	29.515478515688198	22.562798752761825
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	169.0
1	283.0
2	397.0
3	1109.0
4	1821.0
5	1821.0
6	3124.5
7	4428.0
8	4894.0
9	5360.0
10	8208.5
11	11057.0
12	11057.0
13	20570.0
14	30083.0
15	49265.5
16	68448.0
17	67362.0
18	66276.0
19	66276.0
20	71257.5
21	76239.0
22	62961.5
23	49684.0
24	55043.5
25	60403.0
26	60403.0
27	66294.0
28	72185.0
29	77166.5
30	82148.0
31	90369.5
32	98591.0
33	98591.0
34	109532.5
35	120474.0
36	132630.5
37	144787.0
38	153409.5
39	162032.0
40	162032.0
41	167824.5
42	173617.0
43	180254.5
44	186892.0
45	197284.5
46	207677.0
47	207677.0
48	270704.0
49	333731.0
50	310136.5
51	286542.0
52	257520.0
53	228498.0
54	228498.0
55	199735.5
56	170973.0
57	144363.5
58	117754.0
59	105663.0
60	93572.0
61	93572.0
62	83225.5
63	72879.0
64	63635.0
65	54391.0
66	46162.5
67	37934.0
68	37934.0
69	32457.0
70	26980.0
71	22687.0
72	18394.0
73	14948.0
74	11502.0
75	11502.0
76	9207.5
77	6913.0
78	5248.0
79	3583.0
80	2856.5
81	2130.0
82	2130.0
83	1498.5
84	867.0
85	669.5
86	472.0
87	369.0
88	266.0
89	266.0
90	205.0
91	144.0
92	91.0
93	38.0
94	25.0
95	12.0
96	12.0
97	7.5
98	3.0
99	2.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3090348.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.556623488176875
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.55923495611333	30.568734311495405
2	13.622445180454019	11.322056507049146
3	5.132556244541613	6.398751221589206
4	2.402644664231044	3.993831987493065
5	1.3336343025651998	2.771066929130968
6	0.819444532970998	2.043200875567242
7	0.5293408723427793	1.5398333530196384
8	0.3915370983478579	1.3016767822156166
9	0.2693135801594521	1.0072586745845362
>10	1.6607246461942127	13.121863536363291
>50	0.15765162607509844	4.580684109112324
>100	0.1070556247462976	8.631519220110945
>500	0.007600093000144565	2.1118888926874297
>1k	0.005876360567102984	4.974844544857469
>5k	4.701088453682387E-4	1.53778696770758
>10k+	4.701088453682387E-4	4.095002087016011
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	33738	1.0917217090114122	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	31313	1.0132515820224777	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	28318	0.9163369303392369	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11177	0.3616744780846688	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	10919	0.35332590374935124	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	10302	0.333360514738146	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	9219	0.2983159178189641	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	9138	0.2956948537834574	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	9138	0.2956948537834574	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	8616	0.2788035522213032	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	5981	0.1935380740292032	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	5137	0.16622723395552863	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4993	0.1615675645590723	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	4681	0.15147161420008362	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	4443	0.1437702161698294	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4114	0.13312416595153684	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	3976	0.12865864944659955	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3960	0.12814090840254885	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	3741	0.12105432786210485	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	3690	0.11940402828419323	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3514	0.11370887679963551	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	3333	0.10785193123881195	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	3095	0.10015053320855773	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	9.70764457595067E-5	0.0	0.0	0.0
3	0.0	9.70764457595067E-5	0.0	0.0	0.0
4	0.0	1.294352610126756E-4	0.0	9.70764457595067E-5	0.0
5	0.0	2.588705220253512E-4	0.0	9.70764457595067E-5	0.0
6	6.47176305063378E-5	2.912293372785201E-4	0.0	9.70764457595067E-5	0.0
7	9.70764457595067E-5	3.2358815253168897E-4	0.0	9.70764457595067E-5	0.0
8	9.70764457595067E-5	3.2358815253168897E-4	0.0	1.294352610126756E-4	0.0
9	9.70764457595067E-5	3.2358815253168897E-4	0.0	2.265117067721823E-4	0.0
10	9.70764457595067E-5	3.2358815253168897E-4	0.0	2.265117067721823E-4	0.0
11	9.70764457595067E-5	3.2358815253168897E-4	0.0	3.2358815253168897E-4	0.0
12	9.70764457595067E-5	3.559469677848579E-4	0.0	5.500998593038713E-4	0.0
13	9.70764457595067E-5	3.559469677848579E-4	0.0	6.14817489810209E-4	0.0
14	9.70764457595067E-5	6.14817489810209E-4	0.0	8.089703813292224E-4	0.0
15	1.294352610126756E-4	6.471763050633779E-4	0.0	0.0010678409033545737	0.0
16	1.294352610126756E-4	7.118939355697158E-4	0.0	0.0018120936541774584	0.0
17	1.294352610126756E-4	7.118939355697158E-4	0.0	0.002815216927025694	0.0
18	1.294352610126756E-4	7.118939355697158E-4	0.0	0.0032358815253168897	0.0
19	1.6179407626584449E-4	7.118939355697158E-4	0.0	0.004012493091392943	0.0
20	1.6179407626584449E-4	8.736880118355603E-4	0.0	0.005500998593038713	0.0
21	1.6179407626584449E-4	8.736880118355603E-4	0.0	0.010128309174241866	0.0
22	1.6179407626584449E-4	8.736880118355603E-4	0.0	0.015758743028293255	0.0
23	1.6179407626584449E-4	8.736880118355603E-4	0.0	0.020483130055255913	0.0
24	1.6179407626584449E-4	0.0010031232728482358	0.0	0.026534228507598498	0.0
25	1.6179407626584449E-4	0.0010354820881014048	0.0	0.03087030975152313	0.0
26	1.6179407626584449E-4	0.0010354820881014048	0.0	0.03886293711905585	0.0
27	1.6179407626584449E-4	0.0010354820881014048	0.0	0.10070063306786162	0.0
28	1.6179407626584449E-4	0.0010354820881014048	0.0	0.17418750250780818	0.0
29	1.6179407626584449E-4	0.0010354820881014048	0.0	0.26129743316933884	0.0
30	1.6179407626584449E-4	0.0010354820881014048	0.0	0.40438811421885174	0.0
31	1.6179407626584449E-4	0.0010354820881014048	0.0	0.596793629714194	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8450	0.0	29.90651	1
GTATTGG	1165	0.0	22.07296	1
ATTGGAC	1285	0.0	19.291828	3
GTCGCTA	50	0.0070375074	18.499998	16
TTGGACC	1875	0.0	17.661335	4
GTATCAA	14605	0.0	17.353645	2
TGGACCC	1870	0.0	17.114973	5
GGACCCT	1835	0.0	16.93733	6
ACGTTTA	210	0.0	16.738094	26
TAATACT	920	0.0	16.288042	4
CTAATAC	745	0.0	15.892617	3
TATTGGA	1565	0.0	15.840256	2
CGACGAG	235	0.0	15.744681	24
ATACTGG	765	0.0	15.477124	6
GACCCTC	2030	0.0	15.310345	7
AATGCGT	185	3.0559022E-10	15.0	35
GCGACGA	235	0.0	14.957447	23
TACTGGT	660	0.0	14.856061	7
CAATGCG	225	1.8189894E-12	14.8	19
AATACTG	855	0.0	14.71345	5
>>END_MODULE