FastQCFastQC Report
Wed 25 May 2016
SRR2088333_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088333_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3116399
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT379791.2186822034020675No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT365521.1728921745899674No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT328391.0537482523900181No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130680.419330130705343No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA129980.41708394849311653No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG118580.38050326675114454No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG108950.3496022171743734No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA108870.34934551063583325No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA107540.34507776443260313No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG104880.33654227202614295No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA71360.22898223237781812No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA59830.1919844025107183No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA59190.18993075020239705No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC52360.16801442947453135No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA48320.15505074927825352No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA48110.15437689461458562No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT45770.14686822836228608No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG45380.14561678398690284No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG44320.1422154223512458No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG43320.13900659061949386No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC39630.1271660015293292No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC38390.1231870501819568No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT35500.11391352647719372No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA35120.11269417041912798No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA93950.029.9111231
GTATTGG11850.022.1687761
ATTGGAC13300.019.4736843
TACGTTA1552.1827873E-1117.90322719
GGACCCT19950.017.7117796
GTATCAA162300.017.337342
TTGGACC21200.017.2783014
ATTACGT1708.54925E-1116.3235317
TGGACCC21550.016.3109055
TCTAACG1151.2437886E-616.0869562
ATTAGAC2750.015.4727283
TATTGGA16750.015.4626872
GACCCTC22000.015.2204557
GATCGAT1001.0943183E-414.79999926
ACGTTTA1252.962497E-614.79999926
ACGCGAA1051.6570668E-414.09523834
CGTTTAT2151.9826984E-1013.7674421
GATACTG10300.013.65048536
AATTACG2051.4260877E-913.53658516
TATACCG2202.8558134E-1013.4545455
Produced by FastQC (version 0.11.5)