FastQCFastQC Report
Wed 25 May 2016
SRR2088332_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088332_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1013574
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT154251.5218425097723502No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT146461.4449857632496492No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT122431.2079039122945143No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51880.5118521193321849No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA42870.42295875782133324No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA42060.4149672347554298No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG41610.4105274997188168No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG39200.3867502520782893No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA38740.3822118562630849No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG35360.3488645130991916No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA21330.2104434407354569No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA21030.2074836173777149No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC19120.18863940866675744No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT18850.18597556764478962No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA18740.18489029908028423No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA18360.181141189493811No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA17410.17176841552762798No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG14520.14325545051471328No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG13840.13654651757049807No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG13690.13506660589162706No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13260.13082419241219684No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC12980.1280616906116376No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC12830.12658177893276662No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA12760.12589115348262683No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT12560.12391793791079883No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA11650.11493980705898138No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG11020.10872417800772316No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10760.10615899776434676No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC10140.10004202949167995No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34550.030.4138951
GTATTGG3400.024.4852941
GGTCTAG400.001931030823.1251
ATTGGAC3850.019.7012983
GCGAACT1002.8740942E-718.523
CGCTAGA500.00703432218.51
GTATCAA59050.017.9204062
CGAACTA953.6055353E-617.52631624
CGCCGGA852.7226011E-517.41176414
TTGGACC6050.017.1239664
GGGTCGG650.001579760117.07692324
GCGTGCG650.001579760117.0769239
TGGACCC6400.017.0546885
GGACCCT6550.016.6641226
TATTGGA4750.016.3578952
GTCGTAA700.0025922115.8571431
TGCGTTA700.0025922115.85714337
TACTCCG1059.340842E-615.8571425
GACCCTC6300.015.5634937
CGTGTCT1101.4514524E-515.13636435
Produced by FastQC (version 0.11.5)