##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088332_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1013574
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.682222511627174	31.0	30.0	31.0	28.0	34.0
2	30.981877001580546	31.0	31.0	34.0	28.0	34.0
3	31.009367840927254	31.0	31.0	34.0	27.0	34.0
4	34.91566871289121	35.0	35.0	37.0	32.0	37.0
5	34.66607075556397	35.0	35.0	37.0	32.0	37.0
6	34.75036159175354	35.0	35.0	37.0	32.0	37.0
7	34.545560560945724	35.0	35.0	37.0	30.0	37.0
8	34.640825435538005	35.0	35.0	37.0	30.0	37.0
9	36.05124144857702	38.0	35.0	39.0	31.0	39.0
10	35.83080367097025	37.0	35.0	39.0	30.0	39.0
11	35.950226623808426	37.0	35.0	39.0	30.0	39.0
12	35.690413329465834	37.0	35.0	39.0	30.0	39.0
13	35.781751505070176	37.0	35.0	39.0	30.0	39.0
14	36.51438572812641	38.0	35.0	40.0	30.0	41.0
15	36.642198793576	38.0	35.0	40.0	30.0	41.0
16	36.590633737645206	38.0	35.0	40.0	30.0	41.0
17	36.69381909954281	38.0	36.0	40.0	31.0	41.0
18	36.71502426068545	38.0	36.0	40.0	31.0	41.0
19	36.81823823420885	39.0	36.0	40.0	31.0	41.0
20	36.80143235718359	39.0	36.0	40.0	30.0	41.0
21	36.713754496464986	38.0	35.0	40.0	30.0	41.0
22	36.64550787608996	38.0	35.0	40.0	30.0	41.0
23	36.505596039361706	38.0	35.0	40.0	30.0	41.0
24	36.416912825309254	38.0	35.0	40.0	30.0	41.0
25	36.278577587822895	38.0	35.0	40.0	30.0	41.0
26	36.002541501656516	38.0	34.0	40.0	29.0	41.0
27	35.847980512523016	38.0	34.0	40.0	29.0	41.0
28	35.659498961102	38.0	34.0	40.0	27.0	41.0
29	35.43880762529425	38.0	34.0	40.0	27.0	41.0
30	35.20049646103787	37.0	33.0	40.0	27.0	41.0
31	34.91020882540397	37.0	33.0	40.0	26.0	41.0
32	34.7921651502505	37.0	33.0	40.0	26.0	41.0
33	34.592379046818486	37.0	33.0	40.0	25.0	41.0
34	34.725450731767	37.0	33.0	40.0	25.0	41.0
35	34.62272118266649	37.0	33.0	40.0	25.0	41.0
36	34.54900678194192	37.0	33.0	40.0	25.0	41.0
37	34.407651538022876	37.0	33.0	40.0	24.0	41.0
38	34.34771807485196	37.0	33.0	40.0	24.0	41.0
39	34.20297876622723	37.0	33.0	40.0	23.0	41.0
40	33.984519137231224	37.0	33.0	40.0	22.0	41.0
41	33.83845086791887	37.0	33.0	40.0	21.0	41.0
42	33.633371613715425	37.0	32.0	40.0	20.0	41.0
43	33.23355670133606	37.0	31.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	0.0
14	2.0
15	7.0
16	12.0
17	43.0
18	135.0
19	286.0
20	629.0
21	1167.0
22	2159.0
23	3728.0
24	5781.0
25	8819.0
26	13126.0
27	18517.0
28	24847.0
29	32640.0
30	40816.0
31	48359.0
32	57548.0
33	67976.0
34	79819.0
35	93390.0
36	112979.0
37	139341.0
38	157397.0
39	104049.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.773021012772624	23.59393591390466	15.0211035405407	21.611939532782017
2	19.413678724987026	24.727153616805484	34.84826958860429	21.010898069603208
3	20.665585344533305	25.606517136390632	33.38118381095016	20.3467137081259
4	14.335805772444834	17.17417771174083	35.839415770333495	32.650600745480844
5	11.729483984395811	39.59908206011599	35.251792173043114	13.41964178244509
6	31.624232665794505	37.58393565738663	13.240671130080289	17.55116054673857
7	23.986704473477023	35.02201121970374	21.76920481385671	19.222079492962525
8	26.46378064157131	35.02161657658937	19.321233575446882	19.19336920639243
9	23.734626184175994	14.545065283837195	23.352710310248685	38.36759822173813
10	16.1477109712759	27.959379384238346	34.197108449901044	21.695801194584707
11	31.82411940322068	22.620351350764718	24.70584288862974	20.849686357384858
12	20.678707228085962	27.741043081215576	31.83970780623812	19.740541884460335
13	31.84365423738178	21.311418801192612	28.045707565505822	18.799219395919785
14	19.82687006572781	23.74626815604978	29.07256894908512	27.35429282913729
15	21.296126380510945	30.10179819135061	27.32587852490297	21.27619690323548
16	20.453661992118978	29.21858690140039	28.463042658947447	21.864708447533186
17	20.615367008230283	27.07291228859462	28.148808079133836	24.16291262404126
18	22.497420020639836	25.119231550927708	31.661427779323464	20.721920649108995
19	21.623384183098622	24.316034152415117	33.37881595226397	20.681765712222294
20	22.8339519364151	23.831905711867115	33.43317804126783	19.900964310449954
21	21.861847284954035	24.698541991013975	34.051287819142956	19.388322904889037
22	19.682726668205774	25.16589809920144	33.39923873343239	21.752136499160397
23	21.287641553552085	25.601288115125286	34.62598685443786	18.485083476884768
24	21.06121506668482	25.201317318715756	31.873548453294976	21.863919161304455
25	20.72024341587294	26.19611394925284	32.73860615998437	20.345036474889845
26	20.54048347727941	26.940016219832003	32.68315880241601	19.836341500472585
27	19.183305806976104	26.955012658177896	32.41785996878373	21.443821566062272
28	20.08269746461531	26.3468676189405	32.47488589880956	21.095549017634628
29	20.227235505251713	25.88513517513275	31.449701748466317	22.43792757114922
30	20.441625377130826	26.382385499233408	33.498886119809704	19.677103003826065
31	21.833136998383935	25.79446591960725	33.15436268096853	19.218034401040278
32	21.07315302089438	26.0922241493961	31.74519078034756	21.08943204936196
33	20.518383462874937	26.24396442686967	32.37306797530323	20.86458413495216
34	19.4196970324811	27.095505606892047	33.36184629834625	20.122951062280602
35	18.930043588331984	29.542391576737366	31.78090598219765	19.746658852733002
36	21.866780323883603	26.464964570914407	31.3397936411155	20.328461464086487
37	20.772829610862157	27.789978827396915	31.2193288304554	20.21786273128553
38	20.771152377626105	25.671731911039547	32.6843427317591	20.872772979575245
39	20.112493019749913	25.5196956512302	33.50608835664687	20.86172297237301
40	18.419375398342893	26.31845331470618	32.780438330107124	22.481732956843803
41	20.638946934313626	25.286658892197313	32.75606911779505	21.31832505569401
42	19.123320053592536	27.91221953207166	31.808826982539017	21.15563343179679
43	19.490239489173952	28.256841631691422	30.487857817978757	21.765061061155873
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	62.0
1	103.5
2	145.0
3	352.5
4	560.0
5	560.0
6	1104.5
7	1649.0
8	1860.0
9	2071.0
10	3349.5
11	4628.0
12	4628.0
13	8976.5
14	13325.0
15	22253.0
16	31181.0
17	29968.5
18	28756.0
19	28756.0
20	30877.0
21	32998.0
22	26331.0
23	19664.0
24	21314.0
25	22964.0
26	22964.0
27	24574.5
28	26185.0
29	26985.0
30	27785.0
31	30102.5
32	32420.0
33	32420.0
34	35320.0
35	38220.0
36	41580.5
37	44941.0
38	46946.0
39	48951.0
40	48951.0
41	50623.0
42	52295.0
43	54186.5
44	56078.0
45	62005.5
46	67933.0
47	67933.0
48	94452.0
49	120971.0
50	109778.5
51	98586.0
52	86893.0
53	75200.0
54	75200.0
55	63508.5
56	51817.0
57	42149.5
58	32482.0
59	28450.0
60	24418.0
61	24418.0
62	21315.0
63	18212.0
64	15721.0
65	13230.0
66	11105.0
67	8980.0
68	8980.0
69	7650.0
70	6320.0
71	5337.0
72	4354.0
73	3589.0
74	2824.0
75	2824.0
76	2238.0
77	1652.0
78	1273.0
79	894.0
80	667.5
81	441.0
82	441.0
83	316.5
84	192.0
85	145.5
86	99.0
87	77.0
88	55.0
89	55.0
90	39.5
91	24.0
92	16.0
93	8.0
94	5.5
95	3.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1013574.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.39398286348171
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.09501069850815	42.19189573016476
2	10.309798380903347	10.597232026281963
3	3.010442402328983	4.641558757103835
4	1.3127683068945812	2.6987356747304805
5	0.7157645579497933	1.8392995712779618
6	0.44026565024467185	1.3576203170432577
7	0.3149556303910429	1.133077698975204
8	0.24419610141162032	1.0040168201022288
9	0.1824400331170205	0.8438687942066269
>10	1.2053479732230663	11.694687161914095
>50	0.09785058087268819	3.4647530877817427
>100	0.057237137351217736	5.383010575409044
>500	0.008121485635567784	3.1148635607833244
>1k	0.005027586345827675	5.31463719183137
>5k	1.9336870560875675E-4	0.5155828144511976
>10k+	5.801061168262702E-4	4.20516021794294
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	15425	1.5218425097723502	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	14646	1.4449857632496492	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	12243	1.2079039122945143	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5188	0.5118521193321849	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	4287	0.42295875782133324	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	4206	0.4149672347554298	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	4161	0.4105274997188168	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	3920	0.3867502520782893	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3874	0.3822118562630849	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	3536	0.3488645130991916	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2133	0.2104434407354569	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	2103	0.2074836173777149	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	1912	0.18863940866675744	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1885	0.18597556764478962	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1874	0.18489029908028423	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	1836	0.181141189493811	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1741	0.17176841552762798	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	1452	0.14325545051471328	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	1384	0.13654651757049807	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	1369	0.13506660589162706	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1326	0.13082419241219684	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	1298	0.1280616906116376	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	1283	0.12658177893276662	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1276	0.12589115348262683	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	1256	0.12391793791079883	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	1165	0.11493980705898138	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG	1102	0.10872417800772316	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1076	0.10615899776434676	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	1014	0.10004202949167995	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	9.866077859140033E-5	0.0	0.0	0.0	0.0
7	9.866077859140033E-5	0.0	0.0	0.0	0.0
8	9.866077859140033E-5	0.0	0.0	0.0	0.0
9	9.866077859140033E-5	0.0	0.0	0.0	0.0
10	9.866077859140033E-5	0.0	0.0	2.95982335774201E-4	0.0
11	9.866077859140033E-5	0.0	0.0	8.87947007322603E-4	0.0
12	9.866077859140033E-5	0.0	0.0	0.001775894014645206	0.0
13	9.866077859140033E-5	0.0	0.0	0.0019732155718280068	0.0
14	1.9732155718280066E-4	0.0	0.0	0.0022691979076022075	0.0
15	1.9732155718280066E-4	0.0	0.0	0.0027625018005592093	0.0
16	1.9732155718280066E-4	0.0	0.0	0.004341074258021615	0.0
17	3.946431143656013E-4	0.0	0.0	0.005130360486752817	0.0
18	5.91964671548402E-4	0.0	0.0	0.006215629051258221	0.0
19	5.91964671548402E-4	0.0	0.0	0.007300897615763625	0.0
20	5.91964671548402E-4	0.0	0.0	0.010260720973505635	0.0
21	6.906254501398023E-4	0.0	0.0	0.01805492248222626	0.0
22	6.906254501398023E-4	0.0	0.0	0.0292035904630545	0.0
23	8.87947007322603E-4	0.0	0.0	0.03660314885740952	0.0
24	8.87947007322603E-4	0.0	0.0	0.04706119138809796	0.0
25	8.87947007322603E-4	0.0	0.0	0.05238887343203358	0.0
26	8.87947007322603E-4	0.0	0.0	0.06097236116948541	0.0
27	8.87947007322603E-4	0.0	0.0	0.12894963761896022	0.0
28	8.87947007322603E-4	0.0	0.0	0.22238139494501635	0.0
29	8.87947007322603E-4	0.0	0.0	0.3297243220524599	0.0
30	9.866077859140034E-4	0.0	0.0	0.5171798013761205	0.0
31	9.866077859140034E-4	0.0	0.0	0.7650156771977181	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3455	0.0	30.413895	1
GTATTGG	340	0.0	24.485294	1
GGTCTAG	40	0.0019310308	23.125	1
ATTGGAC	385	0.0	19.701298	3
GCGAACT	100	2.8740942E-7	18.5	23
CGCTAGA	50	0.007034322	18.5	1
GTATCAA	5905	0.0	17.920406	2
CGAACTA	95	3.6055353E-6	17.526316	24
CGCCGGA	85	2.7226011E-5	17.411764	14
TTGGACC	605	0.0	17.123966	4
GGGTCGG	65	0.0015797601	17.076923	24
GCGTGCG	65	0.0015797601	17.076923	9
TGGACCC	640	0.0	17.054688	5
GGACCCT	655	0.0	16.664122	6
TATTGGA	475	0.0	16.357895	2
GTCGTAA	70	0.00259221	15.857143	1
TGCGTTA	70	0.00259221	15.857143	37
TACTCCG	105	9.340842E-6	15.857142	5
GACCCTC	630	0.0	15.563493	7
CGTGTCT	110	1.4514524E-5	15.136364	35
>>END_MODULE