FastQCFastQC Report
Wed 25 May 2016
SRR2088331_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088331_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3394695
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT411231.2113901248860355No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT409261.2055869525833691No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT350011.0310499175920076No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145960.4299649894909557No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA117600.3464228745144998No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG112940.3326955735345885No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG112740.3321064189860945No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA108110.3184674911884573No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG103300.30429832429717546No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA97270.2865353146600799No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA67300.19825050556824694No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA66870.19698382328898473No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA58840.17332926816694874No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC58450.17218041679738533No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT53240.1568329408091154No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA49870.14690568666699072No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA47640.14033661345128207No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG42810.12610853110515083No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG42430.12498913746301213No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG41370.1218666183559937No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA39030.11497351013861334No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38160.11241068785266423No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC36360.10710829691621782No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT35630.10495788281421453No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC35370.10419198190117226No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA103300.029.4961281
GTATTGG10050.019.5124381
TCTATAC2750.018.1636353
ATTGGAC11550.018.0995673
ACGTTTA2350.017.31914926
GTATCAA178650.017.1796822
TTGGACC17900.017.0530744
GGACCCT17850.016.478996
CGAATTA2250.015.62222215
TGGACCC19800.015.5101015
GACCCTC18350.015.1226167
TTTTTAC23300.014.45064451
TGCGCTA1551.2122655E-714.3225815
GTTCGTA800.006301964613.87511
ACCGTCG1601.7864659E-713.87523
CCGAATT2700.013.70370414
ATAGCAC2457.2759576E-1213.5918363
TAATACT8250.013.4545464
TATACTG5650.013.4247795
TATTGGA14750.013.4203392
Produced by FastQC (version 0.11.5)