FastQCFastQC Report
Wed 25 May 2016
SRR2088330_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088330_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3171679
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT394021.2423073078959126No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT379241.1957073840070196No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT326831.030463675548503No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135920.4285427371433238No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG107630.3393470776834604No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA106540.3359104121192592No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG105930.3339871405649815No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA103090.3250328926729344No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG96140.30312020857091776No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA93760.29561629660504735No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA65110.2052855916377414No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA57650.1817649264001811No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC54890.17306291084312125No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA54520.17189633629380527No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT49020.1545553632634324No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA47940.15115022674110462No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA47800.15070882015487697No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG39790.12545405761427939No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG39740.12529641240491235No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA38330.12085081750076222No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37960.11968424295144622No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG37580.11848613936025683No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT34620.10915354296572888No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC34060.10738791662081817No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC32610.10281620554917442No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95950.030.579471
ATTGGAC7550.022.2980143
GTATTGG8700.019.7758621
GTATCAA166450.017.7053172
CGAATTA3050.017.59016415
TTGGACC15400.016.4577924
GGACCCT15750.015.6222226
GATACTG11450.015.34934436
GACCCTC16300.015.2085897
GACCGTC1601.0984877E-815.0312522
TGATACT11400.014.92982435
ACCGTCG1752.242814E-914.823
AATGCGT3000.014.79999935
GCTAGCG750.004106524414.79999920
TGGACCC16850.014.7121665
TATACCG1904.5656634E-1014.6052645
ACGTTTA2301.8189894E-1214.47826126
CGAACTA1803.3378456E-914.38888824
TATTGGA12600.014.2420642
TACGTTA3150.014.09523919
Produced by FastQC (version 0.11.5)