##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088330_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3171679
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.933405303626248	31.0	31.0	33.0	28.0	34.0
2	31.250846633596904	31.0	31.0	34.0	28.0	34.0
3	31.316012433792952	31.0	31.0	34.0	28.0	34.0
4	35.20717796473098	35.0	35.0	37.0	33.0	37.0
5	34.96192300671033	35.0	35.0	37.0	32.0	37.0
6	35.00634238206325	36.0	35.0	37.0	32.0	37.0
7	34.818183365971144	35.0	35.0	37.0	32.0	37.0
8	34.8841452744745	36.0	35.0	37.0	32.0	37.0
9	36.357614373964076	38.0	35.0	39.0	32.0	39.0
10	36.1596290166817	38.0	35.0	39.0	31.0	39.0
11	36.25897166768768	38.0	35.0	39.0	32.0	39.0
12	36.01376652555319	38.0	35.0	39.0	30.0	39.0
13	36.09725637430522	38.0	35.0	39.0	31.0	39.0
14	36.921598308025494	39.0	36.0	40.0	31.0	41.0
15	37.056409239396544	39.0	36.0	40.0	31.0	41.0
16	37.030870084898254	39.0	36.0	40.0	31.0	41.0
17	37.10280012573782	39.0	36.0	40.0	31.0	41.0
18	37.11825913025877	39.0	36.0	40.0	31.0	41.0
19	37.20694401924028	39.0	36.0	40.0	31.0	41.0
20	37.192242342305136	39.0	36.0	40.0	31.0	41.0
21	37.12969786665044	39.0	36.0	40.0	31.0	41.0
22	37.055153752949145	39.0	36.0	40.0	31.0	41.0
23	36.93849818976006	39.0	35.0	40.0	31.0	41.0
24	36.852595108143035	39.0	35.0	40.0	31.0	41.0
25	36.7191812286174	38.0	35.0	40.0	30.0	41.0
26	36.487491010281936	38.0	35.0	40.0	30.0	41.0
27	36.35665116173484	38.0	35.0	40.0	30.0	41.0
28	36.19218306770641	38.0	35.0	40.0	30.0	41.0
29	35.97093306100649	38.0	34.0	40.0	29.0	41.0
30	35.76204401517304	38.0	34.0	40.0	29.0	41.0
31	35.522110528839775	38.0	34.0	40.0	27.0	41.0
32	35.409016170930286	38.0	34.0	40.0	27.0	41.0
33	35.226376944198954	38.0	34.0	40.0	27.0	41.0
34	35.37755050243105	38.0	34.0	40.0	27.0	41.0
35	35.2925945532319	38.0	34.0	40.0	27.0	41.0
36	35.222924829404235	38.0	34.0	40.0	26.0	41.0
37	35.111024161020076	38.0	34.0	40.0	26.0	41.0
38	35.06722401604954	38.0	33.0	40.0	25.0	41.0
39	34.958149610978914	38.0	33.0	40.0	25.0	41.0
40	34.75560137075662	38.0	33.0	40.0	24.0	41.0
41	34.63177673402636	38.0	33.0	40.0	23.0	41.0
42	34.47382537766275	38.0	33.0	40.0	23.0	41.0
43	34.10306875317458	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	4.0
14	8.0
15	15.0
16	37.0
17	88.0
18	261.0
19	586.0
20	1303.0
21	2684.0
22	4709.0
23	8145.0
24	13668.0
25	21348.0
26	32298.0
27	46938.0
28	64833.0
29	85802.0
30	109449.0
31	133875.0
32	162537.0
33	195788.0
34	235236.0
35	278486.0
36	339558.0
37	428436.0
38	515512.0
39	490073.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.99916132748616	22.81441469959602	14.493553729743772	22.692870243174042
2	18.190050128023675	24.071919005674914	35.93752709527036	21.800503771031053
3	19.397517844649474	25.353290796451972	33.74367330363508	21.505518055263472
4	13.27650118438846	16.768216455700593	36.961054381606715	32.99422797830424
5	11.933805407167624	38.8880463628255	35.724926765917985	13.453221464088895
6	31.00461931992487	37.881576288142654	14.024149354332515	17.08965503759996
7	24.16726282830009	34.5909532458991	22.342456471793014	18.899327454007796
8	25.956189135155228	34.868724104803796	19.927804799918277	19.2472819601227
9	23.833181100609487	14.682538806732964	23.515841294153663	37.96843879850388
10	15.691405088598184	27.890022918460538	34.80273382016276	21.615838172778517
11	31.68987151600146	22.854645757026486	25.046702393274984	20.408780333697074
12	20.601958773255426	27.53576260397096	31.86580356965506	19.996475053118555
13	30.965744011294966	21.727545568135994	27.836612721526986	19.470097699042054
14	19.734058837606202	23.839613025151664	29.09276127880533	27.333566858436807
15	21.30890295014092	30.097087378640776	27.188722440070386	21.405287231147916
16	20.722494300337456	29.260054374985618	28.15196619834479	21.86548512633214
17	20.4577134066846	27.336152239870426	28.179900929444624	24.02623342400035
18	22.20019743486021	25.920718963047644	31.394696625982643	20.4843869761095
19	21.50715756544089	25.209360720299877	32.71563736431083	20.567844349948402
20	22.65314365041355	24.701459384761193	32.67912673382142	19.96627023100383
21	21.567378035419097	25.5594276722203	33.20373846155301	19.669455830807596
22	19.858535494922407	25.911386366653122	32.69151764727767	21.538560491146804
23	21.26006446427901	26.083282702946924	33.753951771285806	18.90270106148825
24	21.028704355011968	25.842558468243475	31.345921198204486	21.782815978540075
25	20.797596478079907	26.57759502143817	32.27079411251895	20.35401438796297
26	20.45597930938156	27.499062799230312	31.95938176593533	20.085576125452796
27	19.15840159108157	27.546324832998547	31.757028375191815	21.538245200728067
28	20.02472507463712	26.91092005212381	31.7508802120265	21.313474661212563
29	20.15263839751753	26.68164085962041	31.03715729113823	22.128563451723835
30	20.487130002752487	27.01096170198813	32.8368350012722	19.66507329398719
31	21.539254130068016	26.623438248322103	32.45287432933787	19.38443329227201
32	20.918384237496923	26.75144615832813	31.21747188161223	21.112697722562718
33	20.39988914388877	26.69598657367281	31.96458405784444	20.939540224593976
34	19.509067594797582	27.487996105532748	32.624329259045446	20.378607040624225
35	19.101901548044427	29.653568346607585	31.26728776777221	19.977242337575777
36	21.58481359557509	27.177340455954084	30.837673043205193	20.400172905265634
37	20.803492408910234	27.89881952114322	30.77597070825894	20.52171736168761
38	20.42385121571256	26.28137967303753	32.16870307493287	21.12606603631704
39	19.978503499250714	25.9858579635581	32.95084401668643	21.084794520504754
40	18.62010625917692	26.42370176805408	32.56899579055762	22.387196182211376
41	20.521370542227004	25.494225613626092	32.50927978524939	21.47512405889751
42	18.96421422218327	27.853386171803642	31.77200467008168	21.41039493593141
43	19.32664055851806	28.01235560092935	30.797032108230372	21.86397173232222
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	211.0
1	368.5
2	526.0
3	1412.5
4	2299.0
5	2299.0
6	3846.5
7	5394.0
8	6012.5
9	6631.0
10	10245.5
11	13860.0
12	13860.0
13	25722.5
14	37585.0
15	60866.5
16	84148.0
17	82529.0
18	80910.0
19	80910.0
20	88141.5
21	95373.0
22	80751.5
23	66130.0
24	72884.0
25	79638.0
26	79638.0
27	84490.0
28	89342.0
29	92745.5
30	96149.0
31	104849.0
32	113549.0
33	113549.0
34	122700.0
35	131851.0
36	141620.0
37	151389.0
38	156700.5
39	162012.0
40	162012.0
41	167296.0
42	172580.0
43	177961.0
44	183342.0
45	195345.5
46	207349.0
47	207349.0
48	278669.0
49	349989.0
50	318981.5
51	287974.0
52	255690.5
53	223407.0
54	223407.0
55	192579.5
56	161752.0
57	133800.5
58	105849.0
59	91953.5
60	78058.0
61	78058.0
62	67914.0
63	57770.0
64	50370.5
65	42971.0
66	36622.0
67	30273.0
68	30273.0
69	25500.5
70	20728.0
71	17128.5
72	13529.0
73	11107.0
74	8685.0
75	8685.0
76	6835.5
77	4986.0
78	3868.0
79	2750.0
80	2105.5
81	1461.0
82	1461.0
83	1051.5
84	642.0
85	492.5
86	343.0
87	239.5
88	136.0
89	136.0
90	114.0
91	92.0
92	51.5
93	11.0
94	6.5
95	2.0
96	2.0
97	2.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3171679.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.61369310612021
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.14653703920652	27.47230546795135
2	14.015301084516329	10.823650697347746
3	5.830374780860067	6.753969074453805
4	2.940992673786079	4.542503541316942
5	1.5914370221251823	3.0725630385029814
6	0.9775554821067924	2.2648216428166243
7	0.6456466614212241	1.7451561427377174
8	0.46088924924930497	1.4237308821138246
9	0.33207752366209786	1.1540465627515755
>10	1.758186603387989	12.662597825427776
>50	0.16015268729504595	4.281824009003995
>100	0.12316591497181849	9.044844971479495
>500	0.009211753814405275	2.3856025703310793
>1k	0.007320054370354596	5.7624961176414935
>5k	4.934868114845795E-4	1.3404484755010857
>10k+	6.579824153127727E-4	5.269438980622649
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	39402	1.2423073078959126	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	37924	1.1957073840070196	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	32683	1.030463675548503	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13592	0.4285427371433238	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	10763	0.3393470776834604	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	10654	0.3359104121192592	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	10593	0.3339871405649815	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	10309	0.3250328926729344	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	9614	0.30312020857091776	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	9376	0.29561629660504735	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	6511	0.2052855916377414	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	5765	0.1817649264001811	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	5489	0.17306291084312125	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	5452	0.17189633629380527	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4902	0.1545553632634324	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	4794	0.15115022674110462	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	4780	0.15070882015487697	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	3979	0.12545405761427939	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	3974	0.12529641240491235	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3833	0.12085081750076222	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3796	0.11968424295144622	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	3758	0.11848613936025683	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	3462	0.10915354296572888	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3406	0.10738791662081817	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	3261	0.10281620554917442	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	3.1529041873405225E-5	0.0
3	0.0	0.0	0.0	3.1529041873405225E-5	0.0
4	0.0	0.0	0.0	3.1529041873405225E-5	0.0
5	0.0	3.1529041873405225E-5	0.0	6.305808374681045E-5	0.0
6	3.1529041873405225E-5	3.1529041873405225E-5	0.0	6.305808374681045E-5	0.0
7	3.1529041873405225E-5	3.1529041873405225E-5	0.0	9.458712562021567E-5	0.0
8	6.305808374681045E-5	3.1529041873405225E-5	0.0	9.458712562021567E-5	0.0
9	6.305808374681045E-5	3.1529041873405225E-5	0.0	1.5764520936702612E-4	0.0
10	6.305808374681045E-5	3.1529041873405225E-5	0.0	2.2070329311383655E-4	0.0
11	6.305808374681045E-5	6.305808374681045E-5	0.0	3.4681946060745745E-4	0.0
12	6.305808374681045E-5	6.305808374681045E-5	0.0	5.990517955946992E-4	0.0
13	6.305808374681045E-5	6.305808374681045E-5	0.0	6.621098793415097E-4	0.0
14	6.305808374681045E-5	6.305808374681045E-5	0.0	7.882260468351306E-4	0.0
15	6.305808374681045E-5	6.305808374681045E-5	0.0	0.0011035164655691827	0.0
16	6.305808374681045E-5	6.305808374681045E-5	0.0	0.0014503359261766402	0.0
17	9.458712562021567E-5	6.305808374681045E-5	0.0	0.0022070329311383655	0.0
18	9.458712562021567E-5	6.305808374681045E-5	0.0	0.0026484395173660387	0.0
19	9.458712562021567E-5	6.305808374681045E-5	0.0	0.003247491312960738	0.0
20	9.458712562021567E-5	6.305808374681045E-5	0.0	0.00488700149037781	0.0
21	9.458712562021567E-5	6.305808374681045E-5	0.0	0.008134492803338547	0.0
22	9.458712562021567E-5	6.305808374681045E-5	0.0	0.012895378126222736	0.0
23	9.458712562021567E-5	6.305808374681045E-5	0.0	0.0163005146485505	0.0
24	9.458712562021567E-5	6.305808374681045E-5	0.0	0.021818096976396414	0.0
25	9.458712562021567E-5	6.305808374681045E-5	0.0	0.026515924215533792	0.0
26	9.458712562021567E-5	6.305808374681045E-5	0.0	0.03632145623816282	0.0
27	9.458712562021567E-5	6.305808374681045E-5	0.0	0.11917977828147173	0.0
28	1.261161674936209E-4	6.305808374681045E-5	0.0	0.21773956317773646	0.0
29	1.261161674936209E-4	1.261161674936209E-4	0.0	0.32642017051536426	0.0
30	1.261161674936209E-4	1.261161674936209E-4	0.0	0.5043385538069899	0.0
31	1.261161674936209E-4	1.261161674936209E-4	0.0	0.7485309831165133	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9595	0.0	30.57947	1
ATTGGAC	755	0.0	22.298014	3
GTATTGG	870	0.0	19.775862	1
GTATCAA	16645	0.0	17.705317	2
CGAATTA	305	0.0	17.590164	15
TTGGACC	1540	0.0	16.457792	4
GGACCCT	1575	0.0	15.622222	6
GATACTG	1145	0.0	15.349344	36
GACCCTC	1630	0.0	15.208589	7
GACCGTC	160	1.0984877E-8	15.03125	22
TGATACT	1140	0.0	14.929824	35
ACCGTCG	175	2.242814E-9	14.8	23
AATGCGT	300	0.0	14.799999	35
GCTAGCG	75	0.0041065244	14.799999	20
TGGACCC	1685	0.0	14.712166	5
TATACCG	190	4.5656634E-10	14.605264	5
ACGTTTA	230	1.8189894E-12	14.478261	26
CGAACTA	180	3.3378456E-9	14.388888	24
TATTGGA	1260	0.0	14.242064	2
TACGTTA	315	0.0	14.095239	19
>>END_MODULE