FastQCFastQC Report
Wed 25 May 2016
SRR2088329_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088329_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1169745
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT147711.2627538480609022No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT143431.2261646769167638No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT120751.0322762653398816No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50430.4311196029903953No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG38550.32955900645012376No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA36990.3162227665003911No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA36900.31545336804175267No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG36060.3082723157611274No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG35850.30647705269097114No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA31340.2679216410414236No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA23600.20175337359851936No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC19910.17020803679434407No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA19910.17020803679434407No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT18980.1622575860550804No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA18840.1610607440083095No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA17740.15165698506939546No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA15910.13601254974374757No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14290.12216337748825598No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG13970.11942773852420827No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG13730.1173760093011725No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG13280.11352901700798038No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA13240.11318706213747441No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT11850.10130413038739211No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAT251.233639E-437.035
GGTATCA37300.030.1058981
GACGCGA400.001931207123.12500233
ACGTTTA903.8289727E-922.61111326
CGAATTA1059.822543E-1021.14285715
ACGCGAA450.003825847520.55555734
TACCCGG450.003825847520.55555720
CTATCCG450.003825847520.55555737
ATTGGAC3150.020.5555553
ACGGTGT759.266003E-619.73333429
GTATTGG3050.018.196721
GTATCAA62200.018.0241152
GTGCTAG1054.797439E-717.6190471
TTGGACC5400.017.4722214
CCGAATT1401.869921E-917.17857214
TGGACTA650.001579956717.0769235
CGCCGGA1005.877968E-616.65000214
GTGCGGT803.3826285E-416.187511
GGACCCT5450.015.9541286
CCCGAAT1751.3096724E-1015.85714313
Produced by FastQC (version 0.11.5)