FastQCFastQC Report
Wed 25 May 2016
SRR2088328_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088328_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences539688
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT39460.7311631905841893No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT39310.7283838069403062No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT31960.5921940083900328No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15180.281273624760973No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG8640.1600924978876684No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA7440.13785742873660337No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG7120.13192807696298603No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG6870.12729577088984748No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA6320.11710469752894265No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA5510.10209602585197373No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACAG250.005493792229.59999822
CTTCCGT358.8632735E-426.4285735
GGTATCA10550.026.127961
ACGTTTA551.9002344E-523.54545426
TAAGCCG551.9002344E-523.54545436
CGTTTAT551.9002344E-523.54545427
GACCGTG400.001929867323.1257
GTAATAC400.001929867323.1253
CCCGTAT400.001929867323.1251
TTACCCC802.7190254E-823.1254
TCACGTT652.6784674E-622.7692324
CAATGCG450.003823217220.55555719
TAATACT1254.110916E-1019.244
GAGTAAT701.2176769E-418.51
AAGCCGT801.6140824E-518.537
CACGTTT701.2176769E-418.525
TACCCCA1002.8679096E-718.4999985
CGACGAG500.007030165318.49999824
CGTTTAG500.007030165318.49999826
CTAATAC1201.0381518E-716.9583323
Produced by FastQC (version 0.11.5)