##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088328_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	539688
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.920050103022486	31.0	31.0	33.0	28.0	34.0
2	31.273713701249612	31.0	31.0	34.0	28.0	34.0
3	31.366183795081604	31.0	31.0	34.0	28.0	34.0
4	35.25912749588651	35.0	35.0	37.0	33.0	37.0
5	34.93932049628674	35.0	35.0	37.0	32.0	37.0
6	35.00675946102192	36.0	35.0	37.0	32.0	37.0
7	34.84692266642949	35.0	35.0	37.0	32.0	37.0
8	34.8821318984302	35.0	35.0	37.0	32.0	37.0
9	36.391394657654054	38.0	35.0	39.0	32.0	39.0
10	36.154300262373816	38.0	35.0	39.0	32.0	39.0
11	36.27795318776775	38.0	35.0	39.0	32.0	39.0
12	36.025635181808745	38.0	35.0	39.0	30.0	39.0
13	36.11907064822638	38.0	35.0	39.0	31.0	39.0
14	36.96165747617142	39.0	36.0	40.0	31.0	41.0
15	37.103811832021464	39.0	36.0	40.0	31.0	41.0
16	37.09962793317621	39.0	36.0	40.0	31.0	41.0
17	37.13658261810527	39.0	36.0	40.0	31.0	41.0
18	37.123862305628435	39.0	36.0	40.0	31.0	41.0
19	37.201490490802094	39.0	36.0	40.0	31.0	41.0
20	37.18189954195758	39.0	36.0	40.0	31.0	41.0
21	37.114701457138196	39.0	36.0	40.0	31.0	41.0
22	37.022411096781845	39.0	36.0	40.0	31.0	41.0
23	36.91722439631787	39.0	36.0	40.0	31.0	41.0
24	36.84998369428262	39.0	35.0	40.0	31.0	41.0
25	36.73567690962186	38.0	35.0	40.0	30.0	41.0
26	36.515735017269236	38.0	35.0	40.0	30.0	41.0
27	36.388541157112996	38.0	35.0	40.0	30.0	41.0
28	36.24033886245386	38.0	35.0	40.0	30.0	41.0
29	36.048476156594184	38.0	34.0	40.0	29.0	41.0
30	35.86466625161204	38.0	34.0	40.0	29.0	41.0
31	35.69537214094069	38.0	34.0	40.0	28.0	41.0
32	35.56288448140407	38.0	34.0	40.0	27.0	41.0
33	35.394589095922086	38.0	34.0	40.0	27.0	41.0
34	35.59940187663984	38.0	34.0	40.0	28.0	41.0
35	35.57505447591942	38.0	34.0	40.0	27.0	41.0
36	35.53163123878982	38.0	34.0	40.0	27.0	41.0
37	35.452346540964406	38.0	34.0	40.0	27.0	41.0
38	35.46328990083159	38.0	34.0	40.0	27.0	41.0
39	35.38593038941018	38.0	34.0	40.0	26.0	41.0
40	35.253750314996815	38.0	34.0	40.0	26.0	41.0
41	35.17063006774284	38.0	34.0	40.0	25.0	41.0
42	35.090572701264435	38.0	34.0	40.0	25.0	41.0
43	34.73692022057189	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	1.0
16	1.0
17	12.0
18	47.0
19	82.0
20	185.0
21	406.0
22	718.0
23	1205.0
24	2051.0
25	3282.0
26	4969.0
27	7131.0
28	10190.0
29	13661.0
30	17784.0
31	21991.0
32	27159.0
33	33038.0
34	39558.0
35	47727.0
36	58318.0
37	72508.0
38	90904.0
39	86759.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.40035724344436	22.97327344688042	13.990861386578912	23.63550792309631
2	17.894598360534232	23.776515319962645	35.748988304353624	22.579898015149492
3	18.990231392952968	25.702628185173655	32.72186893167904	22.585271490194334
4	13.634914987918945	17.216799335912604	37.66546597293251	31.482819703235943
5	12.550028905589897	38.41330546538	35.862943033752835	13.17372259527727
6	30.968077852388788	38.60508293680793	15.295874653503505	15.130964557299773
7	24.74985547205052	33.49639050710781	23.512103289307895	18.241650731533777
8	25.146936748639952	35.40730940839893	20.65860274825455	18.787151094706573
9	24.369635789567308	14.789470953588	23.01904063088303	37.82185262596167
10	15.445405493544417	28.046204473695912	35.582039993477714	20.926350039281953
11	32.298846741080034	23.51803264108151	24.254569306710543	19.92855131112791
12	19.76845881324024	27.3322734617038	32.26030595455152	20.63896177050444
13	29.362891151924813	22.23043684499192	27.112516861594106	21.294155141489156
14	19.88611938749796	23.413157231585657	28.928566134507346	27.772157246409034
15	22.04477401758053	30.445183142852905	25.77970975823068	21.730333081335885
16	21.331398882317192	29.17278131068321	27.386193504395134	22.109626302604468
17	20.686952461422155	28.235387853722894	28.32191933116912	22.755740353685834
18	21.49723543973555	27.339685151420824	30.188738678643958	20.97434073019967
19	21.507426513096455	26.893130845970266	31.163746460918162	20.43569618001512
20	22.041624049450796	26.695979899497484	30.78426794740665	20.47812810364507
21	21.42163620462193	27.132899008315913	30.882843420643034	20.562621366419116
22	20.857791909399506	27.38786113458146	30.585078786261693	21.16926816975734
23	21.35215161352485	27.420472569336358	30.844673218600377	20.382702598538415
24	21.308793228680276	27.43344302634115	29.817783608307018	21.439980136671558
25	21.32676657624405	27.461422155022902	30.538570433287383	20.673240835445664
26	20.86557418360238	28.206482263826505	30.080713301018363	20.847230251552748
27	20.433102088614163	28.17220319888528	30.079972132046663	21.314722580453893
28	20.668793821615452	28.048427980611017	30.035872578230382	21.246905619543142
29	20.650449889565824	28.014148915669796	29.835756955870803	21.499644238893584
30	21.030298987563185	28.169238522998473	30.52207942366701	20.278383065771337
31	21.58339633269593	27.716384281288448	30.348090007559925	20.3521293784557
32	20.980455374216213	27.98746683268852	29.988623056284368	21.043454736810897
33	20.85834778612828	27.731578245208343	30.259149730955663	21.150924237707713
34	20.469604660470495	27.800877544062498	30.84986140140229	20.879656394064718
35	20.343050058552347	28.793302797171698	30.15316256800225	20.7104845762737
36	21.471850402454752	27.711010806243607	29.848912705118515	20.96822608618313
37	20.871132950890146	27.75900149716132	30.177991728554275	21.191873823394257
38	20.702702302070826	27.110849231407773	30.813544121788883	21.37290434473251
39	20.344347104252826	26.8970219830717	31.49708720594121	21.261543706734262
40	19.70675649634604	27.06248795600421	31.338847630482796	21.891907917166957
41	20.424022768710813	26.41155630660678	31.420932094098813	21.743488830583598
42	19.589651798817094	27.647270274677222	30.859681890277347	21.90339603622834
43	19.80107024799514	27.332458753946725	30.54487036954685	22.321600628511288
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	55.0
1	79.0
2	103.0
3	272.5
4	442.0
5	442.0
6	670.0
7	898.0
8	973.5
9	1049.0
10	1591.0
11	2133.0
12	2133.0
13	3555.5
14	4978.0
15	7496.0
16	10014.0
17	9999.5
18	9985.0
19	9985.0
20	11154.5
21	12324.0
22	11490.5
23	10657.0
24	12151.0
25	13645.0
26	13645.0
27	15273.0
28	16901.0
29	18009.0
30	19117.0
31	21005.5
32	22894.0
33	22894.0
34	24985.5
35	27077.0
36	28652.0
37	30227.0
38	31449.5
39	32672.0
40	32672.0
41	33476.0
42	34280.0
43	35012.0
44	35744.0
45	36035.0
46	36326.0
47	36326.0
48	41347.0
49	46368.0
50	42833.5
51	39299.0
52	35511.5
53	31724.0
54	31724.0
55	28744.5
56	25765.0
57	22837.5
58	19910.0
59	18078.0
60	16246.0
61	16246.0
62	14157.0
63	12068.0
64	10481.5
65	8895.0
66	7517.0
67	6139.0
68	6139.0
69	5260.0
70	4381.0
71	3686.5
72	2992.0
73	2452.5
74	1913.0
75	1913.0
76	1514.5
77	1116.0
78	883.0
79	650.0
80	506.5
81	363.0
82	363.0
83	270.5
84	178.0
85	135.5
86	93.0
87	71.0
88	49.0
89	49.0
90	32.0
91	15.0
92	9.0
93	3.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	539688.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.17781408707137
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.5260444279664	52.769929062985256
2	9.955956673512866	12.579911595562669
3	2.8148518018166966	5.335085514534994
4	1.1450801468524332	2.8937464253055767
5	0.6109493967347309	1.9299223701757622
6	0.35896956732561325	1.3607347552448426
7	0.26490984898404607	1.171549763226375
8	0.19040630261896832	0.9623563190294266
9	0.1381472149714098	0.7855055157699371
>10	0.8937001540795216	10.600861157530183
>50	0.06751868514395512	2.8997370193536476
>100	0.03023662578353232	3.5002707249449503
>500	0.0020549163153856342	0.875206779580086
>1k	0.0011742378945060767	2.335182996756275
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3946	0.7311631905841893	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3931	0.7283838069403062	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3196	0.5921940083900328	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1518	0.281273624760973	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	864	0.1600924978876684	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	744	0.13785742873660337	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	712	0.13192807696298603	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	687	0.12729577088984748	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	632	0.11710469752894265	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	551	0.10209602585197373	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.8529224292554216E-4	0.0
3	0.0	0.0	0.0	3.7058448585108433E-4	0.0
4	0.0	0.0	0.0	3.7058448585108433E-4	0.0
5	0.0	0.0	0.0	3.7058448585108433E-4	0.0
6	0.0	0.0	0.0	3.7058448585108433E-4	0.0
7	0.0	0.0	0.0	3.7058448585108433E-4	0.0
8	0.0	0.0	0.0	3.7058448585108433E-4	0.0
9	0.0	0.0	0.0	3.7058448585108433E-4	0.0
10	0.0	0.0	0.0	3.7058448585108433E-4	0.0
11	0.0	0.0	0.0	3.7058448585108433E-4	0.0
12	0.0	0.0	0.0	5.558767287766265E-4	0.0
13	0.0	0.0	0.0	5.558767287766265E-4	0.0
14	0.0	0.0	0.0	5.558767287766265E-4	0.0
15	0.0	0.0	0.0	5.558767287766265E-4	0.0
16	0.0	0.0	0.0	0.001111753457553253	0.0
17	0.0	0.0	0.0	0.0016676301863298795	0.0
18	0.0	1.8529224292554216E-4	0.0	0.0016676301863298795	0.0
19	0.0	1.8529224292554216E-4	0.0	0.0020382146721809637	0.0
20	0.0	1.8529224292554216E-4	0.0	0.002223506915106506	0.0
21	0.0	1.8529224292554216E-4	0.0	0.0038911371014363856	0.0
22	0.0	1.8529224292554216E-4	0.0	0.007782274202872771	0.0
23	0.0	1.8529224292554216E-4	0.0	0.009820488875053735	0.0
24	0.0	1.8529224292554216E-4	0.0	0.013341041490639036	0.0
25	0.0	1.8529224292554216E-4	0.0	0.01723217859207542	0.0
26	0.0	1.8529224292554216E-4	0.0	0.02482916055202265	0.0
27	0.0	1.8529224292554216E-4	0.0	0.08819910763255807	0.0
28	0.0	1.8529224292554216E-4	0.0	0.15898074443011517	0.0
29	0.0	1.8529224292554216E-4	0.0	0.24884748224900313	0.0
30	0.0	1.8529224292554216E-4	0.0	0.3687315634218289	0.0
31	0.0	1.8529224292554216E-4	0.0	0.5823735195149791	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGACAG	25	0.0054937922	29.599998	22
CTTCCGT	35	8.8632735E-4	26.42857	35
GGTATCA	1055	0.0	26.12796	1
ACGTTTA	55	1.9002344E-5	23.545454	26
TAAGCCG	55	1.9002344E-5	23.545454	36
CGTTTAT	55	1.9002344E-5	23.545454	27
GACCGTG	40	0.0019298673	23.125	7
GTAATAC	40	0.0019298673	23.125	3
CCCGTAT	40	0.0019298673	23.125	1
TTACCCC	80	2.7190254E-8	23.125	4
TCACGTT	65	2.6784674E-6	22.76923	24
CAATGCG	45	0.0038232172	20.555557	19
TAATACT	125	4.110916E-10	19.24	4
GAGTAAT	70	1.2176769E-4	18.5	1
AAGCCGT	80	1.6140824E-5	18.5	37
CACGTTT	70	1.2176769E-4	18.5	25
TACCCCA	100	2.8679096E-7	18.499998	5
CGACGAG	50	0.0070301653	18.499998	24
CGTTTAG	50	0.0070301653	18.499998	26
CTAATAC	120	1.0381518E-7	16.958332	3
>>END_MODULE