FastQCFastQC Report
Wed 25 May 2016
SRR2088327_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088327_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1661529
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT283991.7092088070686697No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT278501.6761669522469966No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT242541.4597397938886412No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90950.5473873763262633No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG85740.5160307162860234No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA83590.5030908277857323No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG80050.4817851509061834No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA79560.4788360600386752No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG79200.4766693810339753No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA69590.41883108871407No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA55490.3339694943633244No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA45010.27089506111539435No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC44780.2695107939735027No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA44290.26656170310599453No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA40960.24651992231252057No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA37920.2282235218283882No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT35780.21534381885600554No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA31200.18777884707399028No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG30680.1846491996227571No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG29450.17724637969003249No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG29290.17628341124349922No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG27480.16538983069209146No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT26380.1587694226221751No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC26070.15690367125701687No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25260.15202864349644213No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC24400.14685268809632573No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT23780.14312118536600926No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT23190.13957023921941777No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA21240.12783406127729338No Hit
ACGCACATGCGAGGGTCCAAAAAAAAAAAAAAAAAAAAAAAAA21140.12723220599821009No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT21080.12687109283076012No Hit
ATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGGA21080.12687109283076012No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCCT21000.12638960860749346No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA20990.12632942307958514No Hit
GCCGAGGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAA20460.12313959010044363No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG20300.12217662165391036No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT20230.12175532295855204No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA20050.12067198345620209No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19510.11742196494915226No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19260.11591732675144399No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC18630.1121256384932192No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG18490.11128304110250259No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG18220.10965803184897767No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA18160.10929691868152769No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC17890.10767190942800277No Hit
CTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTG17280.10400059222559462No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAAAAAAA17200.10351910800232798No Hit
GCACATGCGAGGGTCCAAAAAAAAAAAAAAAAAAAAAAAAAAA16820.10123205794181143No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62800.031.756371
GTATTGG4850.023.6494851
ATTGGAC5650.020.6283173
CGACTAT555.144421E-420.18181836
AGCCGTC1202.382876E-1020.04166612
ATTAGAC1202.382876E-1020.0416663
GCGACTA500.007036167718.535
ACGGGCG801.6173768E-518.55
GCCGCGT701.2196123E-418.513
ACGTGCG1205.178663E-918.59
CGAATTA1103.8546204E-818.515
CGTCGAC609.2391786E-418.519
CGCGTCT801.6173768E-518.515
ACGGTGT801.6173768E-518.529
TTGGACC10950.018.0776274
ACTACGT1054.8004404E-717.6190476
GATACCT9100.017.48351736
GTATCAA115100.017.4070382
TAGACTG752.0679648E-417.2666665
TGCGGTC752.0679648E-417.26666612
Produced by FastQC (version 0.11.5)