FastQCFastQC Report
Wed 25 May 2016
SRR2088326_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088326_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3006183
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT415581.38241750419053No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT411731.3696105659568962No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT357591.1895150760948352No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT143820.47841398876914676No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA118470.3940877850749605No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG115950.3857050618674911No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG112500.3742287146191699No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA111820.37196670994413844No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG107910.35896018306270777No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA107230.3566981783876763No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA70910.23588051692129186No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA64960.2160879760147669No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA64300.2138925008890011No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC60000.1995886477968906No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA53080.17656942375098256No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT52620.17503924411787306No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA50220.16705569820599744No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA43490.14466850487811286No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG43160.14357076731522997No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG42430.14114243876703447No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT39620.13179503709521342No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG39570.13162871322204936No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39040.12986568016651015No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC38600.1284020300826663No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG36350.12091745579028289No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC35750.11892156931231398No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33120.11017293358388362No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT31820.10584851288161766No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT30580.1017236808271486No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102650.029.4125651
GTATTGG10450.021.775121
ATTGGAC10650.020.6713623
CTAACGG851.246679E-619.58823427
CGTATAC2250.018.0888883
TTGGACC18550.017.8517514
ACGTTTA2200.017.6590926
TATTGGA12800.017.4882812
TATACCG1800.017.4722215
GGACCCT19200.017.2473966
TATACTG4050.016.9012345
TGGACCC19750.016.5797485
GTATCAA184300.016.4422152
CCCGTAT1708.54925E-1116.3235281
CCGTATA2400.016.18752
GACCCTC20200.015.844067
GTCTAGA1557.221388E-915.51612951
GTATACT2500.014.7999994
CGTGTCT3300.014.57575735
TCTAACG1551.21212E-714.3225812
Produced by FastQC (version 0.11.5)