##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088326_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3006183
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.8818109210251	31.0	31.0	33.0	28.0	34.0
2	31.20139791888917	31.0	31.0	34.0	28.0	34.0
3	31.25469973052206	31.0	31.0	34.0	28.0	34.0
4	35.156770562537275	35.0	35.0	37.0	33.0	37.0
5	34.877665464810356	35.0	35.0	37.0	32.0	37.0
6	34.962343277172415	36.0	35.0	37.0	32.0	37.0
7	34.77249721656998	35.0	35.0	37.0	32.0	37.0
8	34.84046713057722	35.0	35.0	37.0	32.0	37.0
9	36.295522261951454	38.0	35.0	39.0	32.0	39.0
10	36.08675918931083	38.0	35.0	39.0	31.0	39.0
11	36.20823715655368	38.0	35.0	39.0	32.0	39.0
12	35.962112752284206	38.0	35.0	39.0	30.0	39.0
13	36.050393472386745	38.0	35.0	39.0	30.0	39.0
14	36.80147083527516	38.0	36.0	40.0	31.0	41.0
15	36.93866707382751	38.0	36.0	40.0	31.0	41.0
16	36.912781756799234	38.0	36.0	40.0	31.0	41.0
17	37.020546985995196	38.0	36.0	40.0	31.0	41.0
18	37.02820819624088	39.0	36.0	40.0	31.0	41.0
19	37.124897918722844	39.0	36.0	40.0	31.0	41.0
20	37.11997173824747	39.0	36.0	40.0	31.0	41.0
21	37.04404588809131	39.0	36.0	40.0	31.0	41.0
22	36.96593919931022	39.0	36.0	40.0	31.0	41.0
23	36.85565316549258	39.0	35.0	40.0	31.0	41.0
24	36.76459051228751	39.0	35.0	40.0	30.0	41.0
25	36.64214254421637	38.0	35.0	40.0	30.0	41.0
26	36.39773959203415	38.0	35.0	40.0	30.0	41.0
27	36.25905542011248	38.0	35.0	40.0	30.0	41.0
28	36.079959869375884	38.0	34.0	40.0	30.0	41.0
29	35.85566081639075	38.0	34.0	40.0	29.0	41.0
30	35.61685665842698	38.0	34.0	40.0	28.0	41.0
31	35.35732488674176	38.0	34.0	40.0	27.0	41.0
32	35.23492049552539	37.0	34.0	40.0	27.0	41.0
33	35.03690127979567	37.0	33.0	40.0	27.0	41.0
34	35.16419226640561	38.0	33.0	40.0	27.0	41.0
35	35.08022199579999	38.0	33.0	40.0	26.0	41.0
36	34.99189570295621	38.0	33.0	40.0	25.0	41.0
37	34.86627061625989	38.0	33.0	40.0	25.0	41.0
38	34.80194319507495	38.0	33.0	40.0	25.0	41.0
39	34.669304895942794	38.0	33.0	40.0	24.0	41.0
40	34.46400402104596	38.0	33.0	40.0	23.0	41.0
41	34.30118658777593	38.0	33.0	40.0	23.0	41.0
42	34.124295493654245	38.0	33.0	40.0	21.0	41.0
43	33.74114017676236	37.0	33.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	1.0
14	5.0
15	13.0
16	29.0
17	95.0
18	280.0
19	642.0
20	1443.0
21	2638.0
22	4993.0
23	8514.0
24	14196.0
25	21948.0
26	32604.0
27	47236.0
28	64606.0
29	86366.0
30	109167.0
31	132354.0
32	159135.0
33	190505.0
34	226572.0
35	267227.0
36	324117.0
37	406519.0
38	474721.0
39	430255.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.57434294585526	22.238433255726616	14.634870864481636	22.55235293393649
2	18.527548056788294	23.82087184978426	35.77556655732535	21.876013536102093
3	19.62788027209255	25.061082442419508	33.620042425893566	21.690994859594376
4	13.476491617443118	16.162222991747342	36.18209536811299	34.17919002269655
5	11.872830097169732	38.73410234839329	35.47232487177261	13.920742682664361
6	31.737788418070355	36.990096743944065	13.387375286201806	17.884739551783774
7	24.33827215442307	34.53369272595847	21.743719527387388	19.384315592231076
8	26.410567819723553	34.44530821975908	19.479752230652625	19.664371729864747
9	23.749818291168566	14.470842260767224	23.32243246668616	38.45690698137805
10	15.915032451450891	27.539241623014966	34.35256602808279	22.193159897451352
11	31.846198318598702	22.354959761265366	24.815222493108372	20.983619427027563
12	21.073401053761533	27.272291806586622	31.605394615031752	20.048912524620093
13	31.680805859124344	21.325148868182676	27.71853875828584	19.27550651440714
14	19.979821587707736	23.48905572282193	28.915771262095486	27.615351427374847
15	21.416893116619978	29.76408954478154	27.17229789404038	21.646719444558098
16	20.95594313453306	28.7866374069709	27.9091791817065	22.348240276789536
17	20.70196658021152	26.726516649186028	27.76471026547619	24.806806505126268
18	22.805131956371252	25.037697305852635	31.343101867052003	20.814068870724103
19	21.917960416914074	24.359262227216373	32.76264285973276	20.960134496136796
20	23.214255419580248	23.620351788297654	32.84933086242587	20.31606192969623
21	22.035917307762034	24.63003749272749	33.4769373654232	19.85710783408728
22	19.830828662127356	25.089789942927627	32.91173557963704	22.167645815307985
23	21.433026532316894	25.39822093332309	34.12047769546964	19.048274838890382
24	21.29324794930981	25.156552345615683	31.407203087769442	22.142996617305066
25	20.98451757594265	25.980620607594414	32.493497568178654	20.541364248284285
26	20.772720755855513	26.830202951716515	32.16377712201819	20.233299170409786
27	19.14657224793035	26.949091256254192	31.925734394745763	21.978602101069693
28	20.049511290563483	26.095483874401527	32.067375805132286	21.787629029902703
29	20.29530471032535	25.98288261226945	31.044583779497124	22.677228897908076
30	20.721526267695612	26.28845283204648	33.076595802717264	19.913425097540635
31	21.91164010973384	25.75192528199381	32.807683364585586	19.528751243686763
32	21.077193238069672	26.01455067771989	31.336216058703016	21.57204002550743
33	20.626821454315987	25.91828241993252	32.24999941786644	21.204896707885048
34	19.397821090732002	26.855118267916488	33.11691270957224	20.63014793177927
35	19.02442399547865	29.35543178841741	31.37164304368696	20.248501172416983
36	21.869892817569657	26.53517766549808	31.025323474984724	20.569606041947548
37	20.91373013552402	27.63321461135267	30.817784546050593	20.635270707072724
38	20.64242263361878	25.73605798449396	32.33312808967385	21.288391292213415
39	20.166536767721727	25.349354979387485	33.142825969011206	21.34128228387959
40	18.638818727935057	25.972504002584007	32.61102201695639	22.777655252524546
41	20.71853243797866	25.05130259867746	32.55686696385416	21.67329799948972
42	19.05855365425192	27.55268059196662	31.80391879004039	21.584846963741064
43	19.46717814584142	27.810981566990435	30.489128572678375	22.23271171448977
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	208.0
1	333.0
2	458.0
3	1277.5
4	2097.0
5	2097.0
6	3704.0
7	5311.0
8	6052.5
9	6794.0
10	10563.5
11	14333.0
12	14333.0
13	26556.5
14	38780.0
15	63881.0
16	88982.0
17	86134.5
18	83287.0
19	83287.0
20	87492.0
21	91697.0
22	73488.0
23	55279.0
24	59941.5
25	64604.0
26	64604.0
27	68989.0
28	73374.0
29	77031.0
30	80688.0
31	86489.5
32	92291.0
33	92291.0
34	101186.5
35	110082.0
36	119577.5
37	129073.0
38	135751.0
39	142429.0
40	142429.0
41	147224.5
42	152020.0
43	157650.0
44	163280.0
45	179941.5
46	196603.0
47	196603.0
48	275750.0
49	354897.0
50	322755.5
51	290614.0
52	257305.5
53	223997.0
54	223997.0
55	192294.5
56	160592.0
57	132198.0
58	103804.0
59	92593.5
60	81383.0
61	81383.0
62	72116.5
63	62850.0
64	55258.0
65	47666.0
66	39966.0
67	32266.0
68	32266.0
69	27112.0
70	21958.0
71	18216.5
72	14475.0
73	11868.0
74	9261.0
75	9261.0
76	7241.5
77	5222.0
78	3977.5
79	2733.0
80	2078.5
81	1424.0
82	1424.0
83	1080.5
84	737.0
85	549.0
86	361.0
87	266.0
88	171.0
89	171.0
90	117.5
91	64.0
92	47.5
93	31.0
94	17.0
95	3.0
96	3.0
97	3.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3006183.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.44817204987325
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.4959068805038	30.462709933445844
2	13.60418828289564	11.277374730966566
3	5.30605218511544	6.597784916228119
4	2.4902362791798307	4.128629669771273
5	1.3025080181411002	2.6993288216125877
6	0.8538957602461804	2.12354510300046
7	0.5554553320915093	1.6115825719383854
8	0.37372165247199435	1.2392063480337727
9	0.26786860310498595	0.9992397553429203
>10	1.4942679309125089	11.677366619825937
>50	0.14763517733570095	4.271972118496533
>100	0.09372899072215056	7.2417900560417285
>500	0.006702207245455174	1.8710613419720232
>1k	0.006459958790793331	5.703249603888797
>5k	5.652463941944165E-4	1.3926193363274646
>10k+	8.074948488491664E-4	6.702539073107696
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	41558	1.38241750419053	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	41173	1.3696105659568962	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	35759	1.1895150760948352	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14382	0.47841398876914676	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	11847	0.3940877850749605	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	11595	0.3857050618674911	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	11250	0.3742287146191699	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	11182	0.37196670994413844	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	10791	0.35896018306270777	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	10723	0.3566981783876763	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	7091	0.23588051692129186	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	6496	0.2160879760147669	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	6430	0.2138925008890011	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	6000	0.1995886477968906	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	5308	0.17656942375098256	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5262	0.17503924411787306	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	5022	0.16705569820599744	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	4349	0.14466850487811286	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	4316	0.14357076731522997	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	4243	0.14114243876703447	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	3962	0.13179503709521342	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	3957	0.13162871322204936	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3904	0.12986568016651015	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3860	0.1284020300826663	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG	3635	0.12091745579028289	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	3575	0.11892156931231398	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3312	0.11017293358388362	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	3182	0.10584851288161766	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	3058	0.1017236808271486	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	3.32647746328151E-5
4	0.0	0.0	0.0	3.32647746328151E-5	3.32647746328151E-5
5	0.0	0.0	0.0	3.32647746328151E-5	3.32647746328151E-5
6	0.0	0.0	0.0	3.32647746328151E-5	3.32647746328151E-5
7	0.0	0.0	0.0	6.65295492656302E-5	3.32647746328151E-5
8	0.0	0.0	0.0	6.65295492656302E-5	3.32647746328151E-5
9	0.0	0.0	0.0	9.979432389844531E-5	3.32647746328151E-5
10	0.0	0.0	0.0	2.328534224297057E-4	3.32647746328151E-5
11	0.0	0.0	0.0	8.316193658203775E-4	3.32647746328151E-5
12	0.0	0.0	0.0	0.0011310023375157134	3.32647746328151E-5
13	0.0	0.0	0.0	0.0014303853092110493	3.32647746328151E-5
14	0.0	0.0	0.0	0.0018628273794376456	3.32647746328151E-5
15	0.0	0.0	0.0	0.0024615933228283177	3.32647746328151E-5
16	0.0	0.0	0.0	0.0033930070125471404	3.32647746328151E-5
17	0.0	0.0	0.0	0.004756862772492559	3.32647746328151E-5
18	0.0	0.0	0.0	0.005455423039781676	3.32647746328151E-5
19	0.0	0.0	0.0	0.00651989582803176	3.32647746328151E-5
20	0.0	0.0	0.0	0.009746578967414824	3.32647746328151E-5
21	0.0	0.0	0.0	0.017098094161266963	3.32647746328151E-5
22	0.0	0.0	0.0	0.02594652421359578	3.32647746328151E-5
23	0.0	0.0	0.0	0.032033977971400944	3.32647746328151E-5
24	0.0	0.0	0.0	0.04091567279836258	3.32647746328151E-5
25	0.0	0.0	0.0	0.04620477196498018	3.32647746328151E-5
26	0.0	0.0	0.0	0.05788070786109828	3.32647746328151E-5
27	0.0	0.0	0.0	0.14277241272404242	3.32647746328151E-5
28	3.32647746328151E-5	0.0	0.0	0.2548414384619965	3.32647746328151E-5
29	6.65295492656302E-5	0.0	0.0	0.3825781730520065	3.32647746328151E-5
30	6.65295492656302E-5	0.0	0.0	0.5936099033225855	3.32647746328151E-5
31	6.65295492656302E-5	0.0	0.0	0.8733333932099276	3.32647746328151E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10265	0.0	29.412565	1
GTATTGG	1045	0.0	21.77512	1
ATTGGAC	1065	0.0	20.671362	3
CTAACGG	85	1.246679E-6	19.588234	27
CGTATAC	225	0.0	18.088888	3
TTGGACC	1855	0.0	17.851751	4
ACGTTTA	220	0.0	17.65909	26
TATTGGA	1280	0.0	17.488281	2
TATACCG	180	0.0	17.472221	5
GGACCCT	1920	0.0	17.247396	6
TATACTG	405	0.0	16.901234	5
TGGACCC	1975	0.0	16.579748	5
GTATCAA	18430	0.0	16.442215	2
CCCGTAT	170	8.54925E-11	16.323528	1
CCGTATA	240	0.0	16.1875	2
GACCCTC	2020	0.0	15.84406	7
GTCTAGA	155	7.221388E-9	15.5161295	1
GTATACT	250	0.0	14.799999	4
CGTGTCT	330	0.0	14.575757	35
TCTAACG	155	1.21212E-7	14.322581	2
>>END_MODULE