FastQCFastQC Report
Wed 25 May 2016
SRR2088325_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088325_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3285216
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT604801.84097484001052No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT542731.652037491598726No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT517721.575908555175672No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217460.6619351665156872No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG189580.57707012263425No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA179660.5468742390150297No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG172110.523892492913708No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG165950.5051418232469341No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA165810.5047156716635983No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA116260.35388845056154605No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA95000.28917428869212863No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA90840.27651149878729436No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC85240.2594654354538636No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA84830.258217420102666No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA74540.22689527872748702No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT71880.21879839864410744No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG66800.20333518404878095No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG66300.20181321410829606No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG64860.1974299406796996No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA58740.17880102860816457No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA53510.16288122303069266No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50280.15304929721516028No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC48650.14808767520917954No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA48280.14696141745322075No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47510.144617583744874No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG47450.1444349473520158No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA46310.14096485588771027No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC41640.12674965664358143No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT40880.12443626233404438No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC40670.12379703495904075No Hit
ATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGGA40650.12373615616142135No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCCT40170.12227506501855585No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT39910.12148364064950372No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG38720.11786135219114968No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT38650.11764827639948179No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG38290.11655245804233269No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT38230.1163698216494745No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAAAAAAA37040.11274753319112046No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAAAAAAA36620.11146907844111316No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT35380.10769459298871063No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG34950.10638569883989363No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA34100.10379834994106933No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT33850.10303736497082687No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC33160.10093704645295774No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA156650.028.6741141
GTATTGG12600.020.4087311
ATTGGAC13750.019.509093
TCTAACG701.22007266E-418.52
TATTGGA14800.017.02
TTGGACC22200.016.9166664
GTATCAA266800.016.9051722
TATACCG2300.016.8913045
GACGATA904.450004E-516.44444516
GGACCCT21900.016.2191776
CAATGCG1504.6802597E-916.03333319
TGGACCC22900.015.9148475
TTATGCG2700.015.7592594
TATACTG2500.015.545
TTTTTAC29350.015.0647351
TCTATAC1853.0559022E-1015.03
TCGCTTA750.004106565414.829
TTAACGG1508.115421E-814.835
GATACTG13050.014.74329536
ACCGTCC1152.2122063E-514.4782618
Produced by FastQC (version 0.11.5)