FastQCFastQC Report
Wed 25 May 2016
SRR2088324_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088324_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3023679
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT403191.3334418104567318No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT401571.3280840988742522No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT344131.1381168437522633No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137340.45421488193687226No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA118810.39293192167554825No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG117000.38694583651240755No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG112370.3716333645205063No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA111010.3671355325747211No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA108390.3584705916203407No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG103890.3435880594467865No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA68620.22694207949984108No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA65820.2176818372585185No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA61290.20270008820380733No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC58960.1949942437672782No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA52390.17326574679388917No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA50800.1680072520925667No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT49610.1640716491400046No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG43410.1435668270342189No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG40950.13543104277934265No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG40740.13473652461124347No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC40730.13470345231752445No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT38170.1262369451254581No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA37780.12494712567041673No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37190.12299586034099519No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC36170.11962248638165626No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG33360.11032917184661467No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95250.030.5711291
ATTGGAC12000.021.73753
GTATTGG11850.021.0759491
TTGGACC18600.019.0967734
GTATCAA165100.017.7604482
GGACCCT19300.017.2538876
ACGTTTA2200.016.81818226
GACCCTC20350.016.2727287
CGACGAG2200.015.97727324
TGGACCC21500.015.6604645
CGAATTA1802.0190782E-1015.41666715
CGCCGGA1802.0190782E-1015.41666714
GATACTG10100.015.202970536
TATTGGA17200.015.0581392
CTAATAC4300.015.0581393
TATACCG2950.014.4237295
TGCGGTA908.279516E-414.38888936
TGATACT10750.014.2837235
CGTACAC800.00630175413.8753
CCGTATC950.001245895713.6315799
Produced by FastQC (version 0.11.5)