##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088324_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3023679
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.909238381455175	31.0	31.0	33.0	28.0	34.0
2	31.223640472417873	31.0	31.0	34.0	28.0	34.0
3	31.284564929015282	31.0	31.0	34.0	28.0	34.0
4	35.1795094651251	35.0	35.0	37.0	33.0	37.0
5	34.908901374782175	35.0	35.0	37.0	32.0	37.0
6	34.98912781416281	36.0	35.0	37.0	32.0	37.0
7	34.807934638564475	35.0	35.0	37.0	32.0	37.0
8	34.87361489099868	35.0	35.0	37.0	32.0	37.0
9	36.33930222090374	38.0	35.0	39.0	32.0	39.0
10	36.12743085492871	38.0	35.0	39.0	31.0	39.0
11	36.24298544918293	38.0	35.0	39.0	32.0	39.0
12	35.99094414453386	38.0	35.0	39.0	30.0	39.0
13	36.089953993132205	38.0	35.0	39.0	31.0	39.0
14	36.85939711192888	38.0	36.0	40.0	31.0	41.0
15	36.99668913267579	38.0	36.0	40.0	31.0	41.0
16	36.97359640358649	38.0	36.0	40.0	31.0	41.0
17	37.07373997041353	39.0	36.0	40.0	31.0	41.0
18	37.075418720042705	39.0	36.0	40.0	31.0	41.0
19	37.16575403672149	39.0	36.0	40.0	31.0	41.0
20	37.159943565438	39.0	36.0	40.0	31.0	41.0
21	37.0781994384986	39.0	36.0	40.0	31.0	41.0
22	37.00038562294476	39.0	36.0	40.0	31.0	41.0
23	36.89454072340351	39.0	35.0	40.0	31.0	41.0
24	36.80298371619474	39.0	35.0	40.0	30.0	41.0
25	36.684979457144756	38.0	35.0	40.0	30.0	41.0
26	36.44256979659547	38.0	35.0	40.0	30.0	41.0
27	36.30933442339614	38.0	35.0	40.0	30.0	41.0
28	36.134093599221345	38.0	35.0	40.0	30.0	41.0
29	35.91291337473323	38.0	34.0	40.0	29.0	41.0
30	35.68151645726944	38.0	34.0	40.0	28.0	41.0
31	35.432022711405544	38.0	34.0	40.0	27.0	41.0
32	35.31581626224212	38.0	34.0	40.0	27.0	41.0
33	35.13049599511059	37.0	33.0	40.0	27.0	41.0
34	35.261427221606525	38.0	34.0	40.0	27.0	41.0
35	35.17888572166556	38.0	34.0	40.0	26.0	41.0
36	35.092653353745554	38.0	33.0	40.0	26.0	41.0
37	34.96827077212892	38.0	33.0	40.0	25.0	41.0
38	34.90774814390019	38.0	33.0	40.0	25.0	41.0
39	34.79088884765876	38.0	33.0	40.0	24.0	41.0
40	34.58532999038589	38.0	33.0	40.0	24.0	41.0
41	34.439916737193336	38.0	33.0	40.0	23.0	41.0
42	34.262484542836724	38.0	33.0	40.0	22.0	41.0
43	33.885661143262894	37.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	2.0
13	0.0
14	5.0
15	6.0
16	41.0
17	121.0
18	228.0
19	630.0
20	1364.0
21	2562.0
22	4585.0
23	7994.0
24	13153.0
25	20763.0
26	31807.0
27	45233.0
28	63170.0
29	83963.0
30	107849.0
31	130247.0
32	158608.0
33	190091.0
34	227466.0
35	270847.0
36	327279.0
37	411284.0
38	481286.0
39	443094.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.39403653628577	22.484959547623937	14.498066759070655	22.622937157019642
2	18.26106541071324	23.95221185846778	35.89868501252944	21.888037718289542
3	19.637071263186336	25.155018108734428	33.69163856348508	21.51627206459416
4	13.25587140698467	16.316513756916656	36.69331301371607	33.7343018223826
5	11.772413672218512	38.78232444647729	35.75627571577539	13.688986165528814
6	31.365300350996257	37.462276914976755	13.64165971321691	17.53076302081008
7	24.265637986042833	34.45001933075568	21.94178019558293	19.342562487618558
8	26.28807489154768	34.51169915854163	19.6356160822627	19.564609867647988
9	23.739887732791743	14.520721280268175	23.38551810559256	38.35387288134752
10	15.73457367663697	27.642286102459952	34.68016942274627	21.942970798156818
11	31.749699620892297	22.456815025669062	25.018859475493265	20.774625877945375
12	20.886311013834472	27.228849358678612	31.771725768509157	20.113113858977755
13	31.531620916109148	21.26313672846886	27.854047999142768	19.351194356279223
14	19.896887202642873	23.434597389471566	28.983532974234365	27.68498243365119
15	21.299086311741426	29.93274087626365	27.215521224309857	21.55265158768507
16	20.775915697400418	29.043823765684124	28.034391216792525	22.145869320122934
17	20.67276321329083	26.843358703089848	27.941259637679792	24.54261844593953
18	22.627831856490058	25.285157584518725	31.443284819585678	20.64372573940554
19	21.631595152792343	24.51778115335656	32.87376073981398	20.976862954037117
20	23.06140962714627	23.905183056799352	32.93167032611597	20.101736989938416
21	21.880927175139952	24.82442746071921	33.50941022509334	19.785235139047497
22	19.888222261688494	25.241138361578724	32.992192623621754	21.878446753111028
23	21.37836059978589	25.626695161754938	34.067009097195836	18.92793514126334
24	21.21753003543035	25.300933068622694	31.4118330682589	22.06970382768806
25	20.92573318794753	26.211942471406523	32.35736994568537	20.504954394960574
26	20.674648334032813	26.916779195146045	32.19257070608355	20.216001764737594
27	19.15904432977178	27.136478442321422	31.986133448689497	21.718343779217307
28	20.20604700432817	26.260525670879748	31.924982777603045	21.608444547189038
29	20.218118391535608	26.235688378296768	31.083623625391454	22.46256960477617
30	20.58386488777413	26.56313715840868	33.078114442703736	19.774883511113448
31	21.80459632123648	26.039172809018417	32.67959330338968	19.47663756635542
32	21.134816228839107	26.22133500282272	31.184064181416083	21.45978458692209
33	20.719229785966036	26.061397390397588	31.994467666706683	21.224905156929687
34	19.544336551598235	27.13932265958126	32.88361628334225	20.432724505478262
35	19.166221017508803	29.64527649925802	31.218161716240385	19.97034076699279
36	21.89468524932706	26.74004085751166	30.86941437897343	20.49585951418785
37	21.016748140262244	27.866714687637145	30.59627030514813	20.52026686695248
38	20.81497407628257	25.91320044224271	32.07182376171544	21.200001719759275
39	20.318691236735116	25.605098953956425	32.90603929848373	21.170170510824725
40	18.66067793572003	26.385274362787847	32.43558592033083	22.518461781161296
41	20.866004625491	25.35090530443212	32.25623487149264	21.52685519858424
42	19.205742408503017	27.91037011534624	31.556788931629313	21.327098544521427
43	19.63766656447328	28.123950988183598	30.175094644636552	22.063287802706572
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	221.0
1	342.0
2	463.0
3	1259.5
4	2056.0
5	2056.0
6	3561.5
7	5067.0
8	5748.0
9	6429.0
10	9876.0
11	13323.0
12	13323.0
13	25279.0
14	37235.0
15	61436.5
16	85638.0
17	82886.5
18	80135.0
19	80135.0
20	85539.0
21	90943.0
22	74583.0
23	58223.0
24	63683.5
25	69144.0
26	69144.0
27	74360.5
28	79577.0
29	82713.5
30	85850.0
31	91863.0
32	97876.0
33	97876.0
34	106253.0
35	114630.0
36	124501.5
37	134373.0
38	140113.5
39	145854.0
40	145854.0
41	150445.0
42	155036.0
43	161455.5
44	167875.0
45	184008.0
46	200141.0
47	200141.0
48	276729.0
49	353317.0
50	320879.5
51	288442.0
52	257193.5
53	225945.0
54	225945.0
55	192969.5
56	159994.0
57	131741.5
58	103489.0
59	90925.5
60	78362.0
61	78362.0
62	68020.5
63	57679.0
64	50043.5
65	42408.0
66	36168.5
67	29929.0
68	29929.0
69	25053.0
70	20177.0
71	17267.5
72	14358.0
73	11698.5
74	9039.0
75	9039.0
76	7026.0
77	5013.0
78	3791.0
79	2569.0
80	2006.0
81	1443.0
82	1443.0
83	1051.5
84	660.0
85	534.0
86	408.0
87	301.5
88	195.0
89	195.0
90	158.0
91	121.0
92	75.0
93	29.0
94	19.0
95	9.0
96	9.0
97	6.5
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	3023679.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.17453954420041
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.32881579995522	28.334480547405335
2	14.022874565960219	10.986793084151413
3	5.40257570918169	6.349302472796241
4	2.6015025347074614	4.076506556809402
5	1.4429465176332257	2.8263382707594538
6	0.888735317144977	2.088947809549403
7	0.5780519602467701	1.5851443562702758
8	0.39470161836518125	1.2369803325445354
9	0.29886803226335146	1.0537215793558223
>10	1.73256770214648	12.839981963501279
>50	0.1763664630044312	4.7964497763618885
>100	0.11580072780418668	8.340004890994885
>500	0.008011299206117043	2.069000021212628
>1k	0.0068181269839246135	5.685292877391905
>5k	5.11359523794346E-4	1.1948583630646537
>10k+	8.522658729905767E-4	6.536197097830761
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	40319	1.3334418104567318	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	40157	1.3280840988742522	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	34413	1.1381168437522633	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13734	0.45421488193687226	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	11881	0.39293192167554825	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	11700	0.38694583651240755	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	11237	0.3716333645205063	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	11101	0.3671355325747211	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	10839	0.3584705916203407	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	10389	0.3435880594467865	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	6862	0.22694207949984108	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	6582	0.2176818372585185	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	6129	0.20270008820380733	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	5896	0.1949942437672782	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	5239	0.17326574679388917	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	5080	0.1680072520925667	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4961	0.1640716491400046	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	4341	0.1435668270342189	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	4095	0.13543104277934265	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	4074	0.13473652461124347	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	4073	0.13470345231752445	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	3817	0.1262369451254581	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3778	0.12494712567041673	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3719	0.12299586034099519	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	3617	0.11962248638165626	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG	3336	0.11032917184661467	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	3.3072293719009195E-5	0.0	0.0	0.0	0.0
4	3.3072293719009195E-5	0.0	0.0	0.0	0.0
5	6.614458743801839E-5	0.0	0.0	0.0	0.0
6	9.921688115702759E-5	0.0	0.0	0.0	0.0
7	9.921688115702759E-5	0.0	0.0	0.0	0.0
8	1.3228917487603678E-4	0.0	0.0	0.0	0.0
9	1.3228917487603678E-4	0.0	0.0	0.0	0.0
10	1.3228917487603678E-4	0.0	0.0	6.614458743801839E-5	0.0
11	1.3228917487603678E-4	0.0	0.0	2.3150605603306437E-4	0.0
12	1.3228917487603678E-4	0.0	0.0	5.291566995041471E-4	0.0
13	1.3228917487603678E-4	0.0	0.0	6.945181680991931E-4	0.0
14	1.6536146859504598E-4	0.0	0.0	9.92168811570276E-4	0.0
15	1.6536146859504598E-4	0.0	0.0	0.0013228917487603678	0.0
16	1.6536146859504598E-4	0.0	0.0	0.0021496990917355976	0.0
17	1.6536146859504598E-4	0.0	0.0	0.0028111449661157814	0.0
18	1.6536146859504598E-4	0.0	0.0	0.0032741570781819104	0.0
19	1.6536146859504598E-4	0.0	0.0	0.003968675246281104	0.0
20	1.6536146859504598E-4	0.0	0.0	0.006449097275206793	0.0
21	1.6536146859504598E-4	0.0	0.0	0.011972170326281327	0.0
22	1.6536146859504598E-4	0.0	0.0	0.019115785769587314	0.0
23	1.6536146859504598E-4	0.0	0.0	0.024010485240000674	0.0
24	1.6536146859504598E-4	0.0	0.0	0.030889522333554586	0.0
25	1.9843376231405517E-4	0.0	0.0	0.03680946290925723	0.0
26	1.9843376231405517E-4	0.0	0.0	0.046532717262645934	0.0
27	1.9843376231405517E-4	0.0	0.0	0.11704284747157354	0.0
28	1.9843376231405517E-4	0.0	0.0	0.2057758115196752	0.0
29	1.9843376231405517E-4	0.0	0.0	0.3047611866206697	0.0
30	2.3150605603306437E-4	0.0	0.0	0.4740582581682778	0.0
31	2.3150605603306437E-4	0.0	0.0	0.6915416616644823	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9525	0.0	30.571129	1
ATTGGAC	1200	0.0	21.7375	3
GTATTGG	1185	0.0	21.075949	1
TTGGACC	1860	0.0	19.096773	4
GTATCAA	16510	0.0	17.760448	2
GGACCCT	1930	0.0	17.253887	6
ACGTTTA	220	0.0	16.818182	26
GACCCTC	2035	0.0	16.272728	7
CGACGAG	220	0.0	15.977273	24
TGGACCC	2150	0.0	15.660464	5
CGAATTA	180	2.0190782E-10	15.416667	15
CGCCGGA	180	2.0190782E-10	15.416667	14
GATACTG	1010	0.0	15.2029705	36
TATTGGA	1720	0.0	15.058139	2
CTAATAC	430	0.0	15.058139	3
TATACCG	295	0.0	14.423729	5
TGCGGTA	90	8.279516E-4	14.388889	36
TGATACT	1075	0.0	14.28372	35
CGTACAC	80	0.006301754	13.875	3
CCGTATC	95	0.0012458957	13.631579	9
>>END_MODULE