FastQCFastQC Report
Wed 25 May 2016
SRR2088323_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088323_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1762120
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT237671.3487730688034867No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT230941.3105804372006447No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT181731.031314552924886No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83660.4747690282160126No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA69620.39509227521394685No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG66940.37988332236170064No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG62490.354629650648083No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA61400.34844391982384854No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA56340.31972850884162257No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG51640.2930560915261163No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA34560.1961273920050848No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA30930.1755272058656618No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT29170.16553923682836583No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC28550.162020747735682No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA27850.15804826005039385No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA26380.14970603591128867No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA25570.1451093001611695No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG25270.14340680543890313No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG22930.13012734660522554No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21670.1229768687717068No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC21610.12263636982725354No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG21560.12235262070687582No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA20690.11741538601230336No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC19580.11111615553991783No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT18790.10663291943794975No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58000.029.8232771
GTATCAA94400.018.3236242
TATACCG1950.018.0256425
GTATTGG5450.017.6513771
ATTGGAC5600.017.508933
GGACCCT9250.016.66
CGGTTTA904.448121E-516.44444515
TTGGACC9800.016.423474
TGGACCC10150.015.1280795
GACCCTC10150.015.1280797
CGTTTAG1601.0973963E-815.03125126
CGCCGGA1853.0559022E-1015.014
TTATGCG1752.240995E-914.84
TATGCGG2006.184564E-1114.85
GCATACG750.004105561414.7999991
CGACCGC2059.276846E-1114.43902510
ACCGCCG2700.014.38888912
TTGATAC14750.014.17288134
CGGTCGA800.00630037813.87500129
TCGGTCG800.00630037813.87500128
Produced by FastQC (version 0.11.5)