##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088323_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1762120
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.61953896442921	31.0	30.0	31.0	27.0	34.0
2	30.913803259709894	31.0	31.0	34.0	27.0	34.0
3	30.937870860100332	31.0	31.0	34.0	27.0	34.0
4	34.85008512473611	35.0	35.0	37.0	32.0	37.0
5	34.54037920232447	35.0	35.0	37.0	30.0	37.0
6	34.65982339454748	35.0	35.0	37.0	31.0	37.0
7	34.46897316868318	35.0	35.0	37.0	30.0	37.0
8	34.554329444078725	35.0	35.0	37.0	30.0	37.0
9	35.97354833950015	37.0	35.0	39.0	30.0	39.0
10	35.714390052890835	37.0	35.0	39.0	30.0	39.0
11	35.86353483304202	37.0	35.0	39.0	30.0	39.0
12	35.60043810864186	37.0	35.0	39.0	30.0	39.0
13	35.710574194719996	37.0	35.0	39.0	30.0	39.0
14	36.418805756702156	38.0	35.0	40.0	30.0	41.0
15	36.55318082763944	38.0	35.0	40.0	30.0	41.0
16	36.50368249608426	38.0	35.0	40.0	30.0	41.0
17	36.59790536399337	38.0	35.0	40.0	30.0	41.0
18	36.59881336117858	38.0	35.0	40.0	30.0	41.0
19	36.69734467573151	38.0	35.0	40.0	30.0	41.0
20	36.69361621228974	38.0	35.0	40.0	30.0	41.0
21	36.60037681883186	38.0	35.0	40.0	30.0	41.0
22	36.51134826232039	38.0	35.0	40.0	30.0	41.0
23	36.40689510362518	38.0	35.0	40.0	30.0	41.0
24	36.314478015118155	38.0	35.0	40.0	30.0	41.0
25	36.20346230676685	38.0	34.0	40.0	30.0	41.0
26	35.91198726533948	38.0	34.0	40.0	29.0	41.0
27	35.757432524459176	38.0	34.0	40.0	28.0	41.0
28	35.581530769754615	38.0	34.0	40.0	27.0	41.0
29	35.36672303815858	38.0	34.0	40.0	27.0	41.0
30	35.13413104669375	37.0	33.0	40.0	27.0	41.0
31	34.88592434113454	37.0	33.0	40.0	26.0	41.0
32	34.76925351281411	37.0	33.0	40.0	26.0	41.0
33	34.59360599732141	37.0	33.0	40.0	25.0	41.0
34	34.72840782693574	37.0	33.0	40.0	25.0	41.0
35	34.64867148661839	37.0	33.0	40.0	25.0	41.0
36	34.57238667060132	37.0	33.0	40.0	25.0	41.0
37	34.44805688602365	37.0	33.0	40.0	24.0	41.0
38	34.39570006582979	37.0	33.0	40.0	24.0	41.0
39	34.2728486141693	37.0	33.0	40.0	23.0	41.0
40	34.070269902163304	37.0	33.0	40.0	23.0	41.0
41	33.92634553832883	37.0	33.0	40.0	22.0	41.0
42	33.733708828002634	37.0	32.0	40.0	21.0	41.0
43	33.35268483417701	37.0	32.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	3.0
15	10.0
16	26.0
17	100.0
18	230.0
19	544.0
20	1067.0
21	2000.0
22	3831.0
23	6484.0
24	10323.0
25	15855.0
26	23472.0
27	32385.0
28	44326.0
29	57425.0
30	71643.0
31	86339.0
32	101568.0
33	119773.0
34	139570.0
35	163406.0
36	196934.0
37	237977.0
38	272957.0
39	173872.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.25771230109187	23.9596622250471	14.891267337071254	21.891358136789776
2	19.201586725081153	24.911356774793997	34.62641590811069	21.260640592014166
3	20.880076271763556	25.647855991646423	33.098540394524775	20.37352734206524
4	14.47092139014369	17.184300728667743	36.08034640092615	32.26443148026241
5	11.815767371121149	39.634360883481264	35.328978730165936	13.220893015231653
6	31.5193062901505	37.837150704832816	13.552822736249517	17.090720268767168
7	24.340680543890315	34.51688874764488	22.071992826822235	19.070437881642565
8	26.263705082514242	35.10129843597485	19.5423694186548	19.092627062856103
9	23.83078337457154	14.554400381358818	23.253070165482487	38.36174607858715
10	15.792057294622385	28.13735727419245	34.653996322611405	21.416589108573763
11	32.00928427121876	22.651011281865028	24.73543232015981	20.604272126756406
12	20.49531246453136	27.483485801194018	32.044469162145596	19.976732572129027
13	31.468515197602887	21.231073933670803	27.94310262638186	19.35730824234445
14	19.803759108346764	23.521156334415362	29.095691553356183	27.579393003881687
15	21.416191859805235	30.26048169250675	27.03334619662679	21.289980251061223
16	20.59649740085806	29.15278187637618	28.32412094522507	21.92659977754069
17	20.700122579620004	27.19133770685311	28.287347059224118	23.821192654302774
18	22.18662747145484	25.513188659115155	31.459718974871176	20.840464894558828
19	21.53803373209543	24.793771139309467	32.96205706762309	20.70613806097201
20	22.6276303543459	24.358329739177808	33.04088257326402	19.973157333212267
21	21.856967743399995	25.047499602751234	33.56417270106463	19.531359952784147
22	19.891494336367558	25.516196399791163	33.024084625337665	21.568224638503622
23	21.487129139899665	25.766009125371713	33.849737815812766	18.897123918915852
24	21.06286745511089	25.534469843143487	31.59137856672644	21.811284135019182
25	21.005720382266816	26.37323224298005	32.25024402424352	20.370803350509615
26	20.7465439357138	26.981420107597664	32.2526275168547	20.019408439833835
27	19.428415771911105	27.193437450343904	31.98834358613488	21.389803191610106
28	20.40360474882528	26.465734456223185	31.994813066079498	21.13584772887204
29	20.32040950673053	26.17648060291013	31.31460967471001	22.18850021564933
30	20.61176310353438	26.6884775157197	32.98776473792932	19.711994642816606
31	21.791308196944588	26.198215785531065	32.61145665448437	19.399019363039972
32	21.068088438925837	26.36262002587792	31.40274215149933	21.16654938369691
33	20.728837990602226	26.475779175084558	31.865763966131706	20.92961886818151
34	19.742185549224796	27.353017955644336	32.64811704083717	20.256679454293693
35	19.289265203277868	29.65229382788913	31.258030100106687	19.800410868726306
36	21.91933580005902	26.94600821737453	30.933591355866795	20.201064626699658
37	20.941366081765146	28.00615168092979	30.775259346696025	20.27722289060904
38	20.86935055501328	26.276190043810864	31.98578984405148	20.868669557124374
39	20.425850679862894	26.061505459333077	32.69680838989399	20.81583547091004
40	18.935146301046466	26.89538737429914	31.94311397634667	22.22635234830772
41	20.935407350237213	25.954645540598825	31.970240392254784	21.139706716909178
42	19.488230086486734	28.250516423399084	31.095725603250628	21.16552788686355
43	19.929630218146325	28.36254057612421	29.904887294849384	21.802941910880076
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	127.0
1	174.0
2	221.0
3	554.0
4	887.0
5	887.0
6	1729.5
7	2572.0
8	2994.5
9	3417.0
10	5264.5
11	7112.0
12	7112.0
13	14258.5
14	21405.0
15	35241.0
16	49077.0
17	47230.5
18	45384.0
19	45384.0
20	49301.0
21	53218.0
22	44140.5
23	35063.0
24	38494.0
25	41925.0
26	41925.0
27	44985.0
28	48045.0
29	50751.0
30	53457.0
31	57612.0
32	61767.0
33	61767.0
34	67407.0
35	73047.0
36	79053.5
37	85060.0
38	87551.5
39	90043.0
40	90043.0
41	92507.5
42	94972.0
43	97789.5
44	100607.0
45	107626.0
46	114645.0
47	114645.0
48	153596.5
49	192548.0
50	176192.0
51	159836.0
52	142630.5
53	125425.0
54	125425.0
55	107291.0
56	89157.0
57	74147.0
58	59137.0
59	52303.5
60	45470.0
61	45470.0
62	39744.5
63	34019.0
64	29524.0
65	25029.0
66	21246.5
67	17464.0
68	17464.0
69	14786.0
70	12108.0
71	10167.5
72	8227.0
73	6768.5
74	5310.0
75	5310.0
76	4184.0
77	3058.0
78	2301.5
79	1545.0
80	1209.0
81	873.0
82	873.0
83	649.5
84	426.0
85	327.5
86	229.0
87	167.5
88	106.0
89	106.0
90	92.5
91	79.0
92	46.5
93	14.0
94	10.5
95	7.0
96	7.0
97	4.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1762120.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.020629113495005
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.14431730993688	39.58848542601116
2	12.111529246457984	12.11652624868645
3	3.745175845474743	5.620081557939367
4	1.5992580360840936	3.199835723189555
5	0.8351289169010576	2.08868369071313
6	0.485853962012492	1.4581632502289372
7	0.32840662297788203	1.1498974120474417
8	0.24121342962287062	0.9652518000287794
9	0.18118513773620917	0.8156695118022386
>10	1.1419275517745515	10.870676653084642
>50	0.11285485716256156	3.8938129756706306
>100	0.0623082354412252	5.820616925636086
>500	0.004222225173131967	1.518744526772588
>1k	0.005477481305684714	4.600813558346455
>5k	7.987993570790206E-4	2.5805585489105094
>10k+	3.423425816052946E-4	3.7121821909320287
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	23767	1.3487730688034867	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	23094	1.3105804372006447	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	18173	1.031314552924886	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8366	0.4747690282160126	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	6962	0.39509227521394685	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	6694	0.37988332236170064	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	6249	0.354629650648083	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	6140	0.34844391982384854	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5634	0.31972850884162257	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	5164	0.2930560915261163	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3456	0.1961273920050848	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	3093	0.1755272058656618	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2917	0.16553923682836583	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	2855	0.162020747735682	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	2785	0.15804826005039385	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	2638	0.14970603591128867	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	2557	0.1451093001611695	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	2527	0.14340680543890313	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	2293	0.13012734660522554	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2167	0.1229768687717068	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	2161	0.12263636982725354	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	2156	0.12235262070687582	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2069	0.11741538601230336	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	1958	0.11111615553991783	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	1879	0.10663291943794975	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	5.6749824075545367E-5	0.0	0.0	0.0	0.0
3	5.6749824075545367E-5	0.0	0.0	0.0	0.0
4	5.6749824075545367E-5	0.0	0.0	0.0	0.0
5	5.6749824075545367E-5	0.0	0.0	0.0	0.0
6	1.1349964815109073E-4	0.0	0.0	0.0	0.0
7	1.1349964815109073E-4	0.0	0.0	5.6749824075545367E-5	0.0
8	1.702494722266361E-4	0.0	0.0	5.6749824075545367E-5	0.0
9	1.702494722266361E-4	0.0	0.0	5.6749824075545367E-5	0.0
10	1.702494722266361E-4	0.0	0.0	5.6749824075545367E-5	0.0
11	1.702494722266361E-4	0.0	0.0	2.2699929630218147E-4	0.0
12	1.702494722266361E-4	5.6749824075545367E-5	0.0	3.972487685288176E-4	0.0
13	1.702494722266361E-4	5.6749824075545367E-5	0.0	4.5399859260436293E-4	0.0
14	1.702494722266361E-4	5.6749824075545367E-5	0.0	5.107484166799082E-4	0.0
15	1.702494722266361E-4	5.6749824075545367E-5	0.0	7.944975370576351E-4	0.0
16	1.702494722266361E-4	5.6749824075545367E-5	0.0	0.001702494722266361	0.0
17	1.702494722266361E-4	5.6749824075545367E-5	0.0	0.0024402424352484506	0.0
18	1.702494722266361E-4	5.6749824075545367E-5	0.0	0.002780741379701723	0.0
19	1.702494722266361E-4	5.6749824075545367E-5	0.0	0.003858988037137085	0.0
20	1.702494722266361E-4	5.6749824075545367E-5	0.0	0.005674982407554536	0.0
21	1.702494722266361E-4	5.6749824075545367E-5	0.0	0.01089596622250471	0.0
22	1.702494722266361E-4	5.6749824075545367E-5	0.0	0.0171384468708147	0.0
23	1.702494722266361E-4	5.6749824075545367E-5	0.0	0.02031643701904524	0.0
24	1.702494722266361E-4	5.6749824075545367E-5	0.0	0.025934669602524232	0.0
25	1.702494722266361E-4	5.6749824075545367E-5	0.0	0.030417905704492317	0.0
26	1.702494722266361E-4	5.6749824075545367E-5	0.0	0.036206387760197946	0.0
27	1.702494722266361E-4	5.6749824075545367E-5	0.0	0.0845572378725626	0.0
28	1.702494722266361E-4	5.6749824075545367E-5	0.0	0.14794679136494676	0.0
29	1.702494722266361E-4	5.6749824075545367E-5	0.0	0.2222890609039112	0.0
30	1.702494722266361E-4	5.6749824075545367E-5	0.0	0.352132658388759	0.0
31	1.702494722266361E-4	5.6749824075545367E-5	0.0	0.5146074047170454	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5800	0.0	29.823277	1
GTATCAA	9440	0.0	18.323624	2
TATACCG	195	0.0	18.025642	5
GTATTGG	545	0.0	17.651377	1
ATTGGAC	560	0.0	17.50893	3
GGACCCT	925	0.0	16.6	6
CGGTTTA	90	4.448121E-5	16.444445	15
TTGGACC	980	0.0	16.42347	4
TGGACCC	1015	0.0	15.128079	5
GACCCTC	1015	0.0	15.128079	7
CGTTTAG	160	1.0973963E-8	15.031251	26
CGCCGGA	185	3.0559022E-10	15.0	14
TTATGCG	175	2.240995E-9	14.8	4
TATGCGG	200	6.184564E-11	14.8	5
GCATACG	75	0.0041055614	14.799999	1
CGACCGC	205	9.276846E-11	14.439025	10
ACCGCCG	270	0.0	14.388889	12
TTGATAC	1475	0.0	14.172881	34
CGGTCGA	80	0.006300378	13.875001	29
TCGGTCG	80	0.006300378	13.875001	28
>>END_MODULE