FastQCFastQC Report
Wed 25 May 2016
SRR2088322_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088322_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2728369
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT465661.7067339498432945No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT453831.6633747121448748No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT407061.4919536177108008No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157180.5760950956413887No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA155540.5700841784963838No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG147980.5423753165352634No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG147170.5394065098965719No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA144570.5298770071057104No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG141630.5191013385652747No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA129730.4754855373301778No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA88750.3252859125726762No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA84030.3079861998138815No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA79480.29130956992987384No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC73760.27034466378997857No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA66810.24487156979132954No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA65430.23981360292541073No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT58230.2134242105814866No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG56530.2071933818336156No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG55360.20290510557772795No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG53600.19645436522699092No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA49730.1822700668421317No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC48790.17882478506389715No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT46440.1702115806183108No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC44990.16489705021571496No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG43260.15855626566641096No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39780.14580139270018094No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT39690.1454715252958819No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT38940.14272263026005647No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA37900.13891082914371186No Hit
ATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGGA35620.13055418823480255No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT35350.1295645860219054No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG35160.12886819927949628No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCCT35070.12853833187519723No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34550.12663243131702495No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC34550.12663243131702495No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA33680.12344371307546743No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG33050.12113464124537407No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT32920.12065816610583098No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA32690.11981517162817787No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA32680.11977851969436684No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG32150.11783596720238355No Hit
ACGCACATGCGAGGGTCCAAAAAAAAAAAAAAAAAAAAAAAAA31620.11589341471040025No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29830.10933271855823022No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC29600.10848972408057708No Hit
GATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGA29470.108013248941034No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC29460.107976597007223No Hit
GCCGAGGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAA29150.1068403870590818No Hit
CTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTG27620.10123264118599792No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA106600.030.8911821
ATTGGAC10500.022.728573
GTATTGG11250.022.0355571
TTGGACC19350.019.025844
TATTGGA12750.019.0078452
TCACGCA609.241252E-418.54
ATAACGC500.007037299718.53
CGTGTCT3250.018.21538435
GGACCCT20400.017.9558836
GTCGTCA1451.546141E-1017.8620724
GTATCAA191650.017.2402822
GACCCTC20200.017.1262367
TGGACCC21850.017.1029745
CTGTGCG2300.016.8913049
CGTTTAG1554.0199666E-1016.70967926
TATACCG2250.016.4444455
TTTTTAC23350.016.241971
TGTGCGC2750.016.14545410
TATCACG700.002593718515.8571422
TAGGTCG1659.749783E-1015.6969721
Produced by FastQC (version 0.11.5)