FastQCFastQC Report
Wed 25 May 2016
SRR2088321_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088321_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences868575
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT142071.6356676164982875No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT139731.6087269378004203No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT111581.2846328756871888No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47690.5490602423509772No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA38730.445902771781366No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG38010.4376133321820223No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA37050.42656074604956395No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG36170.41642920876147715No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA33740.38845235011369195No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG32360.3725642575482831No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA20730.23866678179777223No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT18110.20850243214460468No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC17940.20654520335031518No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA17720.20401231902829348No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA17330.19952220591198228No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA17070.19652879716777477No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA16880.19434130616239242No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG13730.15807500791526352No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA13440.15473620585441672No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG12930.14886451947154822No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12630.145410586305155No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG12470.14356848861641194No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC12320.14184152203321532No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT11640.13401260685605734No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC10930.12583829836226001No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG10440.1201968741904844No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA10260.11812451429064849No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9590.11041073021903693No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT9380.10799297700256168No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT9380.10799297700256168No Hit
ATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGGA9180.10569035489163285No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC8990.10350286388625046No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC8840.10177589730305384No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT8700.10016406182540367No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCTAG200.001841688437.011
GGTATCA33400.031.6272451
TGCGGTC400.001930809323.12512
GTATTGG3000.022.8166681
GTGTACC450.00382506720.55555511
GGACCCT4900.019.6326526
ACGATCT851.2445907E-619.58823630
GATACTG3800.018.98684336
GTATCAA55850.018.947182
TACCCCG801.6159294E-518.55
CGATCTG801.6159294E-518.531
TGTGTAC500.007033533418.49999810
TATTGGA4100.018.048782
TTGGACC5650.018.008854
TGGACCC5500.017.8272725
TATACTG1156.3995685E-817.6956525
CTTACCC1054.7939284E-717.6190473
GACCCTC5550.017.3333347
ATTGGAC4100.017.1463413
GGTGTAT650.001579513517.0769231
Produced by FastQC (version 0.11.5)