##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088321_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	868575
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.676907578505023	31.0	30.0	31.0	28.0	34.0
2	30.98341421293498	31.0	31.0	34.0	28.0	34.0
3	31.002943902368823	31.0	31.0	34.0	27.0	34.0
4	34.911066977520655	35.0	35.0	37.0	32.0	37.0
5	34.62709265175719	35.0	35.0	37.0	32.0	37.0
6	34.750530466568804	35.0	35.0	37.0	32.0	37.0
7	34.55494113922229	35.0	35.0	37.0	30.0	37.0
8	34.65031574705696	35.0	35.0	37.0	30.0	37.0
9	36.0515890970843	37.0	35.0	39.0	31.0	39.0
10	35.81508447744869	37.0	35.0	39.0	30.0	39.0
11	35.954008577267366	37.0	35.0	39.0	30.0	39.0
12	35.69398267276861	37.0	35.0	39.0	30.0	39.0
13	35.79547534755203	37.0	35.0	39.0	30.0	39.0
14	36.48853697147627	38.0	35.0	40.0	30.0	41.0
15	36.61967245200472	38.0	35.0	40.0	30.0	41.0
16	36.577994991796906	38.0	35.0	40.0	30.0	41.0
17	36.7190305960913	38.0	36.0	40.0	31.0	41.0
18	36.73380767348819	38.0	36.0	40.0	31.0	41.0
19	36.838944247762136	39.0	36.0	40.0	31.0	41.0
20	36.833213021328035	39.0	36.0	40.0	31.0	41.0
21	36.74848113289008	38.0	35.0	40.0	30.0	41.0
22	36.66588032121579	38.0	35.0	40.0	30.0	41.0
23	36.53120455919178	38.0	35.0	40.0	30.0	41.0
24	36.45604006562473	38.0	35.0	40.0	30.0	41.0
25	36.326942405664454	38.0	35.0	40.0	30.0	41.0
26	36.03362634199695	38.0	34.0	40.0	29.0	41.0
27	35.88875456926575	38.0	34.0	40.0	29.0	41.0
28	35.69741933626918	38.0	34.0	40.0	28.0	41.0
29	35.47655527732205	38.0	34.0	40.0	27.0	41.0
30	35.21246869873068	37.0	33.0	40.0	27.0	41.0
31	34.91906398411191	37.0	33.0	40.0	26.0	41.0
32	34.79858964395706	37.0	33.0	40.0	26.0	41.0
33	34.59425956307746	37.0	33.0	40.0	25.0	41.0
34	34.704585096278386	37.0	33.0	40.0	25.0	41.0
35	34.60279883717583	37.0	33.0	40.0	25.0	41.0
36	34.51104855654376	37.0	33.0	40.0	24.0	41.0
37	34.3643508044786	37.0	33.0	40.0	24.0	41.0
38	34.282653771982844	37.0	33.0	40.0	24.0	41.0
39	34.1220654520335	37.0	33.0	40.0	23.0	41.0
40	33.89111705955156	37.0	33.0	40.0	21.0	41.0
41	33.71823273752986	37.0	33.0	40.0	21.0	41.0
42	33.49971850444694	37.0	32.0	40.0	19.0	41.0
43	33.11069165011657	37.0	31.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	1.0
15	2.0
16	10.0
17	47.0
18	91.0
19	249.0
20	532.0
21	1014.0
22	1802.0
23	3153.0
24	5109.0
25	7472.0
26	11180.0
27	15836.0
28	21429.0
29	28087.0
30	34939.0
31	41615.0
32	49464.0
33	58684.0
34	68928.0
35	80204.0
36	97157.0
37	119441.0
38	132422.0
39	89706.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.405923495380364	23.677632904469963	15.370693377083153	21.545750223066516
2	19.298506173905537	24.77218432489998	35.01689548973894	20.912414011455546
3	20.50508016003224	25.378464726707538	33.841406902109775	20.275048211150448
4	14.107359755922054	17.06553838183231	35.866908441988315	32.96019342025732
5	11.302075238177475	39.826612555047056	35.38600581412083	13.485306392654635
6	31.14860547448407	37.724318567769046	13.068071266154332	18.059004691592552
7	23.496186282128775	35.232190657110785	21.82056817200587	19.45105488875457
8	26.43053276919092	34.98983967993553	19.26926287309674	19.310364677776818
9	23.379328209999137	14.51285150965662	23.773767377601242	38.334052902743
10	16.05468727513456	28.131364591428486	34.17275422387244	21.641193909564514
11	31.497107330973144	22.651239098523444	24.863137898281668	20.98851567222174
12	20.623434936533975	28.134127737961606	31.709639351811873	19.532797973692542
13	31.84722102293987	21.411852747316008	28.140690210977752	18.60023601876637
14	19.50309414846156	24.03649656045822	29.59122700975736	26.869182281322857
15	21.04585096278387	30.140172121002788	27.884984025559106	20.928992890654232
16	20.040641280257894	29.19563653109979	28.871081944564374	21.892640244077942
17	20.343436087845035	27.135480528451772	28.42057392856115	24.100509455142046
18	22.127737961603778	25.132659816365887	32.28886394381602	20.450738278214317
19	21.43844803269723	24.318222375730365	33.94514002820712	20.29818956336528
20	22.569726275796565	23.626169300290705	34.211208013124946	19.592896410787787
21	21.516967446679907	24.573468036726823	34.90809659499755	19.001467921595715
22	19.07054658492358	25.14705120455919	34.10885646029416	21.673545750223067
23	20.83849984169473	25.42785597098696	35.55306104826872	18.180583139049592
24	20.64174078231586	25.22948507613044	32.45062314710877	21.678150994444927
25	20.404800967101284	26.128083354920417	33.53676999683389	19.930345681144406
26	20.088420689059667	27.05442823014708	33.320668911723224	19.53648216907003
27	18.62970958178626	27.09288201939959	32.98932158996057	21.28808680885358
28	19.50125205077282	26.264628846098493	33.25389287050629	20.9802262326224
29	19.773652246495697	25.935584146446764	31.971677748035574	22.31908585902196
30	20.07184180986098	26.259908470771094	34.42201306738048	19.24623665198745
31	21.347609590421094	25.825979333966554	33.90265665026048	18.92375442535187
32	20.45258037590306	26.121981406326455	32.4077943758455	21.017643841924993
33	20.018190714676336	26.114152491149294	33.24122844889618	20.626428345278185
34	18.80609043548341	27.014017212100278	34.14880695391878	20.031085398497538
35	18.3615692369686	29.640157729614597	32.43542584117664	19.562847192240163
36	21.58650663442996	26.49512131940247	31.90904642661831	20.009325619549262
37	20.36531099789886	27.793224534438593	31.82108626198083	20.020378205681723
38	20.44486659183145	25.583858619002385	33.383760757562676	20.587514031603487
39	19.615807500791526	25.525372017384797	34.04000805917739	20.81881242264629
40	17.949515010217887	26.265780157153962	33.3682180583139	22.41648677431425
41	20.26491667386236	25.18101488069539	33.301442017096974	21.252626428345277
42	18.657571309328496	28.00126644216101	32.318567769047	21.02259447946349
43	19.24520047203753	28.21564056068848	30.862734939412256	21.676424027861728
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	62.0
1	87.0
2	112.0
3	283.5
4	455.0
5	455.0
6	947.0
7	1439.0
8	1675.5
9	1912.0
10	3010.5
11	4109.0
12	4109.0
13	8140.5
14	12172.0
15	20768.5
16	29365.0
17	28034.5
18	26704.0
19	26704.0
20	28640.5
21	30577.0
22	24315.5
23	18054.0
24	19057.5
25	20061.0
26	20061.0
27	21365.5
28	22670.0
29	23348.0
30	24026.0
31	25839.0
32	27652.0
33	27652.0
34	30238.0
35	32824.0
36	35754.5
37	38685.0
38	40465.5
39	42246.0
40	42246.0
41	43297.5
42	44349.0
43	46070.5
44	47792.0
45	52555.0
46	57318.0
47	57318.0
48	81691.0
49	106064.0
50	95181.0
51	84298.0
52	73672.0
53	63046.0
54	63046.0
55	53000.0
56	42954.0
57	34449.5
58	25945.0
59	22391.0
60	18837.0
61	18837.0
62	16580.5
63	14324.0
64	12298.0
65	10272.0
66	8712.0
67	7152.0
68	7152.0
69	6049.0
70	4946.0
71	4141.5
72	3337.0
73	2774.0
74	2211.0
75	2211.0
76	1735.5
77	1260.0
78	950.0
79	640.0
80	489.5
81	339.0
82	339.0
83	260.0
84	181.0
85	134.0
86	87.0
87	70.0
88	53.0
89	53.0
90	43.0
91	33.0
92	21.5
93	10.0
94	6.0
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	868575.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.60507678684614
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.52827138244795	41.76349510394456
2	9.885100636533672	10.004725535149761
3	2.9557337970644695	4.487254072857713
4	1.2688313997436336	2.568372416543522
5	0.7198032057453414	1.8212848249080502
6	0.4838404266875215	1.4690869167061453
7	0.31940507222670944	1.1314462744298537
8	0.2503289011328944	1.0134330611037548
9	0.1937736629172923	0.8825337982078215
>10	1.2095704679820938	11.63786495489179
>50	0.10847606054061212	3.7765436297437076
>100	0.06140102510248937	5.646939739007891
>500	0.009323859366009445	3.3970941763855387
>1k	0.005457868897176261	5.872844947637346
>5k	0.0	0.0
>10k+	6.822336121470326E-4	4.5270805484824805
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	14207	1.6356676164982875	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	13973	1.6087269378004203	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	11158	1.2846328756871888	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4769	0.5490602423509772	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	3873	0.445902771781366	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	3801	0.4376133321820223	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	3705	0.42656074604956395	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	3617	0.41642920876147715	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3374	0.38845235011369195	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	3236	0.3725642575482831	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	2073	0.23866678179777223	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1811	0.20850243214460468	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	1794	0.20654520335031518	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1772	0.20401231902829348	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	1733	0.19952220591198228	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1707	0.19652879716777477	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1688	0.19434130616239242	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	1373	0.15807500791526352	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1344	0.15473620585441672	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	1293	0.14886451947154822	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1263	0.145410586305155	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	1247	0.14356848861641194	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	1232	0.14184152203321532	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	1164	0.13401260685605734	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	1093	0.12583829836226001	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG	1044	0.1201968741904844	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	1026	0.11812451429064849	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	959	0.11041073021903693	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	938	0.10799297700256168	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	938	0.10799297700256168	No Hit
ATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGGA	918	0.10569035489163285	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	899	0.10350286388625046	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	884	0.10177589730305384	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT	870	0.10016406182540367	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.15131105546441E-4	0.0
5	1.15131105546441E-4	0.0	0.0	1.15131105546441E-4	0.0
6	2.30262211092882E-4	0.0	0.0	1.15131105546441E-4	0.0
7	2.30262211092882E-4	0.0	0.0	1.15131105546441E-4	0.0
8	2.30262211092882E-4	0.0	0.0	1.15131105546441E-4	0.0
9	2.30262211092882E-4	0.0	0.0	1.15131105546441E-4	0.0
10	2.30262211092882E-4	0.0	0.0	5.75655527732205E-4	0.0
11	2.30262211092882E-4	0.0	0.0	0.001036179949917969	0.0
12	2.30262211092882E-4	0.0	0.0	0.0016118354776501742	0.0
13	2.30262211092882E-4	0.0	0.0	0.00230262211092882	0.0
14	2.30262211092882E-4	0.0	0.0	0.0027631465331145843	0.0
15	2.30262211092882E-4	0.0	0.0	0.003914457588578994	0.0
16	2.30262211092882E-4	0.0	0.0	0.004950637538496963	0.0
17	2.30262211092882E-4	0.0	0.0	0.007022997438332902	0.0
18	2.30262211092882E-4	0.0	0.0	0.008174308493797312	0.0
19	2.30262211092882E-4	0.0	0.0	0.009901275076993926	0.0
20	2.30262211092882E-4	0.0	0.0	0.015542699248769536	0.0
21	2.30262211092882E-4	0.0	0.0	0.02740120312005296	0.0
22	2.30262211092882E-4	0.0	0.0	0.04248337794663673	0.0
23	2.30262211092882E-4	0.0	0.0	0.05031229312379472	0.0
24	2.30262211092882E-4	0.0	0.0	0.06228592810062459	0.0
25	2.30262211092882E-4	0.0	0.0	0.06838787669458596	0.0
26	2.30262211092882E-4	0.0	0.0	0.07967072503813719	0.0
27	2.30262211092882E-4	0.0	0.0	0.16152894108165675	0.0
28	2.30262211092882E-4	0.0	0.0	0.2703278358230435	0.0
29	2.30262211092882E-4	0.0	0.0	0.38453789252511295	0.0
30	2.30262211092882E-4	0.0	0.0	0.5958034712028323	0.0
31	2.30262211092882E-4	0.0	0.0	0.8588780473764499	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCTAG	20	0.0018416884	37.0	11
GGTATCA	3340	0.0	31.627245	1
TGCGGTC	40	0.0019308093	23.125	12
GTATTGG	300	0.0	22.816668	1
GTGTACC	45	0.003825067	20.555555	11
GGACCCT	490	0.0	19.632652	6
ACGATCT	85	1.2445907E-6	19.588236	30
GATACTG	380	0.0	18.986843	36
GTATCAA	5585	0.0	18.94718	2
TACCCCG	80	1.6159294E-5	18.5	5
CGATCTG	80	1.6159294E-5	18.5	31
TGTGTAC	50	0.0070335334	18.499998	10
TATTGGA	410	0.0	18.04878	2
TTGGACC	565	0.0	18.00885	4
TGGACCC	550	0.0	17.827272	5
TATACTG	115	6.3995685E-8	17.695652	5
CTTACCC	105	4.7939284E-7	17.619047	3
GACCCTC	555	0.0	17.333334	7
ATTGGAC	410	0.0	17.146341	3
GGTGTAT	65	0.0015795135	17.076923	1
>>END_MODULE