FastQCFastQC Report
Wed 25 May 2016
SRR2088320_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088320_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2960687
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT391141.321112295896189No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT389831.3166876471575686No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT333421.1261575438403317No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133030.4493213906096794No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA109970.37143406243213145No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG106040.3581601162162701No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG105970.35792368460428275No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA102490.34616965589405435No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG99810.3371177027493957No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA97100.32796442177102814No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA66010.22295501010407381No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA60930.2057968302627059No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA57920.19563027094724972No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC55950.18897640986703423No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT49650.16769756478817247No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA48740.16462395383233688No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA47040.15888204325550118No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG41370.13973108268452558No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG40110.13547531366875323No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG38420.12976717903648716No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA37680.12726775913833513No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC36210.12230269528660072No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT36010.12162717639520827No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34980.11814825410453723No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC34180.11544617853896748No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG33050.11162949680260022No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95350.029.9181981
GTATTGG9250.022.41
ATTGGAC9850.020.472083
TTAACGG1254.129106E-1019.24000235
CGTTAGA1002.8787326E-718.52
CGTACAA801.6180735E-518.533
ACGTATA701.22002115E-418.529
TTGGACC16100.018.3850924
GGACCCT17100.017.5263166
CTGTGCG2350.017.3191499
GTATCAA166700.017.1793632
ACGTTTA2050.017.14634326
TATTGGA12100.016.9710732
TGGACCC18350.016.433245
GATACTG11100.016.16666636
TCACGTT1951.8189894E-1216.12820624
TGATACT11400.015.74122835
AGCCGTC2600.015.65384612
TATACTG3800.015.5789485
GACCCTC17950.015.459617
Produced by FastQC (version 0.11.5)