##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088320_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2960687
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.93011723292601	31.0	31.0	33.0	28.0	34.0
2	31.242638617320914	31.0	31.0	34.0	28.0	34.0
3	31.301629993308985	31.0	31.0	34.0	28.0	34.0
4	35.194972315547034	35.0	35.0	37.0	33.0	37.0
5	34.91753197821992	35.0	35.0	37.0	32.0	37.0
6	35.002139030569595	36.0	35.0	37.0	32.0	37.0
7	34.82259353994529	35.0	35.0	37.0	32.0	37.0
8	34.889931965114855	35.0	35.0	37.0	32.0	37.0
9	36.36157418869337	38.0	35.0	39.0	32.0	39.0
10	36.1465686849032	38.0	35.0	39.0	31.0	39.0
11	36.264133628445016	38.0	35.0	39.0	32.0	39.0
12	36.00544164242961	38.0	35.0	39.0	30.0	39.0
13	36.11054934209526	38.0	35.0	39.0	31.0	39.0
14	36.886383802137814	39.0	36.0	40.0	31.0	41.0
15	37.02259610691708	38.0	36.0	40.0	31.0	41.0
16	37.00879593148482	38.0	36.0	40.0	31.0	41.0
17	37.10155751013194	39.0	36.0	40.0	31.0	41.0
18	37.10276635118809	39.0	36.0	40.0	31.0	41.0
19	37.199732697174674	39.0	36.0	40.0	31.0	41.0
20	37.183914071294936	39.0	36.0	40.0	31.0	41.0
21	37.11529013367505	39.0	36.0	40.0	31.0	41.0
22	37.03234350676043	39.0	36.0	40.0	31.0	41.0
23	36.923719731265074	39.0	35.0	40.0	31.0	41.0
24	36.840586998895866	39.0	35.0	40.0	31.0	41.0
25	36.71705080611358	38.0	35.0	40.0	30.0	41.0
26	36.4726281434005	38.0	35.0	40.0	30.0	41.0
27	36.338159690639365	38.0	35.0	40.0	30.0	41.0
28	36.16621851617547	38.0	35.0	40.0	30.0	41.0
29	35.95594434670061	38.0	34.0	40.0	29.0	41.0
30	35.70959037547704	38.0	34.0	40.0	28.0	41.0
31	35.4696301905605	38.0	34.0	40.0	27.0	41.0
32	35.35385638535921	38.0	34.0	40.0	27.0	41.0
33	35.16614826221076	37.0	33.0	40.0	27.0	41.0
34	35.297422186134504	38.0	34.0	40.0	27.0	41.0
35	35.21023026074691	38.0	34.0	40.0	27.0	41.0
36	35.12750587954755	38.0	33.0	40.0	26.0	41.0
37	35.0095156968636	38.0	33.0	40.0	25.0	41.0
38	34.95073440725075	38.0	33.0	40.0	25.0	41.0
39	34.829800988757	38.0	33.0	40.0	24.0	41.0
40	34.63093903543333	38.0	33.0	40.0	24.0	41.0
41	34.48524176990003	38.0	33.0	40.0	23.0	41.0
42	34.31268857532053	38.0	33.0	40.0	22.0	41.0
43	33.93429599278816	37.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	3.0
14	4.0
15	18.0
16	25.0
17	85.0
18	243.0
19	568.0
20	1189.0
21	2403.0
22	4481.0
23	7741.0
24	12767.0
25	19939.0
26	30087.0
27	43396.0
28	60889.0
29	81481.0
30	104142.0
31	126792.0
32	153368.0
33	185596.0
34	222617.0
35	264020.0
36	321969.0
37	403428.0
38	475900.0
39	437536.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.40085966534119	22.359810408867943	14.503795909530456	22.735534016260413
2	18.417211951145124	23.81832324727335	35.78335028322818	21.981114518353344
3	19.646858989146775	25.05198962267879	33.518267888500205	21.78288349967423
4	13.33258125563425	16.259165524758274	36.49264511919024	33.91560810041723
5	11.940269268585297	38.63160813689526	35.62075288607002	13.807369708449425
6	31.570915804338657	37.23281116848894	13.58083444822097	17.61543857895144
7	24.498840978462095	34.27380199257807	21.881847017263222	19.34551001169661
8	26.33696165788548	34.42028826417653	19.585049010584367	19.657701067353624
9	23.901716054415747	14.429792814978414	23.272571534917404	38.39591959568843
10	15.89063619355913	27.551645952442794	34.34979786785972	22.207919986138354
11	31.971768714490928	22.390039879257753	24.830149218745515	20.808042187505805
12	21.014852296105598	27.17004533069521	31.731858180212903	20.083244192986292
13	31.501573790137222	21.30350151839759	27.65983030289929	19.535094388565895
14	20.022109733315276	23.36038899079842	28.928724988490846	27.688776287395463
15	21.44414455158549	29.759782104626392	27.08064040541942	21.715432938368696
16	21.03126740516644	28.78764286802354	27.896025483274663	22.28506424353537
17	20.704518917399913	26.756965528608735	27.80233101303853	24.736184540952827
18	22.720267289314947	25.27305317988697	31.27375504401512	20.732924486782967
19	21.91156984848449	24.505697495209727	32.630534737376834	20.952197918928952
20	23.146452157894434	23.886179119913724	32.633135485108696	20.33423323708315
21	21.989930039886012	24.777323641438624	33.285484078526366	19.94726224014899
22	20.001303751460387	25.246606615288954	32.67900321783424	22.07308641541642
23	21.479609293383596	25.462570004867114	33.93087482736271	19.126945874386585
24	21.361731246835618	25.305072775338967	31.183438168235956	22.149757809589463
25	21.044744007049715	26.10829851314914	32.21650920884241	20.630448270958734
26	20.814358289140326	26.877545650722283	32.002606151882986	20.3054899082544
27	19.369997571509586	26.97667804803412	31.756818603249855	21.89650577720644
28	20.151100065626657	26.235262288786355	31.87858088342334	21.735056762163644
29	20.453732528970473	26.059222065689486	30.849360300497825	22.63768510484222
30	20.742719510708156	26.41741595785032	32.88230062819879	19.95756390324273
31	22.063291391491234	25.95438153374538	32.45902049085229	19.523306583911097
32	21.17917226643681	26.1553821798792	31.09251332545453	21.57293222822946
33	20.679727374085815	26.038652515446586	32.01503569948461	21.266584410982993
34	19.618284540040875	26.895818436734448	32.81228309510597	20.673613928118712
35	19.23928466602515	29.26246509678328	31.150844381726266	20.347405855465304
36	21.989018089382633	26.6114587594028	30.82642643413505	20.573096717079515
37	21.043190313599514	27.652332043204837	30.618940806643863	20.685536836551787
38	20.729242908824876	25.78800123079542	32.06103853598844	21.421717324391267
39	20.33176759312957	25.35506117330201	32.9114154924178	21.40175574115062
40	18.72045238149119	26.110223741989614	32.437809197662574	22.73151467885663
41	20.78976940149364	25.065263568894654	32.41224080762336	21.732726221988344
42	19.17842041391069	27.561035665033152	31.63184760834225	21.628696312713906
43	19.533540695115693	27.7422098317046	30.481405160356363	22.24284431282334
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	179.0
1	305.0
2	431.0
3	1229.0
4	2027.0
5	2027.0
6	3528.0
7	5029.0
8	5718.5
9	6408.0
10	9743.0
11	13078.0
12	13078.0
13	24334.0
14	35590.0
15	59720.5
16	83851.0
17	81207.5
18	78564.0
19	78564.0
20	82834.0
21	87104.0
22	70951.5
23	54799.0
24	59700.0
25	64601.0
26	64601.0
27	70145.5
28	75690.0
29	78949.5
30	82209.0
31	88441.0
32	94673.0
33	94673.0
34	103000.0
35	111327.0
36	120944.5
37	130562.0
38	136439.5
39	142317.0
40	142317.0
41	146861.5
42	151406.0
43	157284.5
44	163163.0
45	179074.0
46	194985.0
47	194985.0
48	267912.0
49	340839.0
50	311873.5
51	282908.0
52	251023.0
53	219138.0
54	219138.0
55	189222.0
56	159306.0
57	132140.5
58	104975.0
59	92749.0
60	80523.0
61	80523.0
62	70893.0
63	61263.0
64	52974.0
65	44685.0
66	37884.5
67	31084.0
68	31084.0
69	26402.5
70	21721.0
71	18212.0
72	14703.0
73	12099.0
74	9495.0
75	9495.0
76	7545.5
77	5596.0
78	4428.5
79	3261.0
80	2465.0
81	1669.0
82	1669.0
83	1218.5
84	768.0
85	586.5
86	405.0
87	318.0
88	231.0
89	231.0
90	155.0
91	79.0
92	54.5
93	30.0
94	22.5
95	15.0
96	15.0
97	7.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2960687.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.92849210371875
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.7595522060591	29.77938757935802
2	14.032852717362612	11.486870032704477
3	5.368375366191158	6.591585263548592
4	2.55086129074892	4.176116247843959
5	1.432840533356704	2.932200122768901
6	0.874348389385031	2.1471456690506665
7	0.5446011738621354	1.5602793390864655
8	0.3863380980192289	1.2649788635316583
9	0.2822633355467981	1.0397351430086796
>10	1.499695327516167	11.536750978205037
>50	0.146103217941599	4.199979971802041
>100	0.10672697527182072	8.202007484125199
>500	0.007720684369349051	2.1090522373201446
>1k	0.006558430808365878	5.805865296883887
>5k	4.981086689898135E-4	1.487287695250812
>10k+	6.64144891986418E-4	5.680758075511469
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	39114	1.321112295896189	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	38983	1.3166876471575686	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	33342	1.1261575438403317	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13303	0.4493213906096794	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	10997	0.37143406243213145	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	10604	0.3581601162162701	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	10597	0.35792368460428275	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	10249	0.34616965589405435	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	9981	0.3371177027493957	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	9710	0.32796442177102814	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	6601	0.22295501010407381	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	6093	0.2057968302627059	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	5792	0.19563027094724972	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	5595	0.18897640986703423	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4965	0.16769756478817247	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	4874	0.16462395383233688	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	4704	0.15888204325550118	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	4137	0.13973108268452558	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	4011	0.13547531366875323	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	3842	0.12976717903648716	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3768	0.12726775913833513	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	3621	0.12230269528660072	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	3601	0.12162717639520827	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3498	0.11814825410453723	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	3418	0.11544617853896748	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG	3305	0.11162949680260022	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	3.377594456962185E-5	0.0	0.0	0.0	0.0
3	6.75518891392437E-5	0.0	0.0	3.377594456962185E-5	0.0
4	6.75518891392437E-5	3.377594456962185E-5	0.0	1.0132783370886555E-4	0.0
5	6.75518891392437E-5	3.377594456962185E-5	0.0	1.0132783370886555E-4	0.0
6	1.351037782784874E-4	3.377594456962185E-5	0.0	1.0132783370886555E-4	0.0
7	1.351037782784874E-4	3.377594456962185E-5	0.0	1.351037782784874E-4	0.0
8	1.351037782784874E-4	3.377594456962185E-5	0.0	1.351037782784874E-4	0.0
9	1.351037782784874E-4	3.377594456962185E-5	0.0	2.3643161198735294E-4	0.0
10	1.351037782784874E-4	3.377594456962185E-5	0.0	3.039835011265966E-4	0.0
11	1.351037782784874E-4	3.377594456962185E-5	0.0	5.741910576835714E-4	0.0
12	1.351037782784874E-4	3.377594456962185E-5	0.0	0.0010132783370886554	0.0
13	1.351037782784874E-4	3.377594456962185E-5	0.0	0.0011821580599367647	0.0
14	2.026556674177311E-4	3.377594456962185E-5	0.0	0.0013510377827848738	0.0
15	2.026556674177311E-4	3.377594456962185E-5	0.0	0.0016887972284810925	0.0
16	2.026556674177311E-4	3.377594456962185E-5	0.0	0.00219543639702542	0.0
17	2.3643161198735294E-4	3.377594456962185E-5	0.0	0.002870955288417857	0.0
18	2.3643161198735294E-4	3.377594456962185E-5	0.0	0.003715353902658403	0.0
19	3.3775944569621846E-4	3.377594456962185E-5	0.0	0.004525976572329327	0.0
20	3.3775944569621846E-4	3.377594456962185E-5	0.0	0.0071267243041902096	0.0
21	3.715353902658403E-4	3.377594456962185E-5	0.0	0.012902410825595546	0.0
22	3.715353902658403E-4	3.377594456962185E-5	0.0	0.020873533744026302	0.0
23	3.715353902658403E-4	3.377594456962185E-5	0.0	0.02664922026543164	0.0
24	3.715353902658403E-4	3.377594456962185E-5	0.0	0.03384349645876109	0.0
25	3.715353902658403E-4	3.377594456962185E-5	0.0	0.03914631975619172	0.0
26	4.728632239747059E-4	6.75518891392437E-5	0.0	0.04981951824019223	0.0
27	4.728632239747059E-4	6.75518891392437E-5	0.0	0.13530643394590514	0.0
28	4.728632239747059E-4	6.75518891392437E-5	0.0	0.23264870619555528	0.0
29	4.728632239747059E-4	6.75518891392437E-5	0.0	0.34627098372776316	0.0
30	5.066391685443277E-4	6.75518891392437E-5	0.0	0.538321004550633	0.0
31	5.404151131139496E-4	6.75518891392437E-5	0.0	0.7867430768602017	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9535	0.0	29.918198	1
GTATTGG	925	0.0	22.4	1
ATTGGAC	985	0.0	20.47208	3
TTAACGG	125	4.129106E-10	19.240002	35
CGTTAGA	100	2.8787326E-7	18.5	2
CGTACAA	80	1.6180735E-5	18.5	33
ACGTATA	70	1.22002115E-4	18.5	29
TTGGACC	1610	0.0	18.385092	4
GGACCCT	1710	0.0	17.526316	6
CTGTGCG	235	0.0	17.319149	9
GTATCAA	16670	0.0	17.179363	2
ACGTTTA	205	0.0	17.146343	26
TATTGGA	1210	0.0	16.971073	2
TGGACCC	1835	0.0	16.43324	5
GATACTG	1110	0.0	16.166666	36
TCACGTT	195	1.8189894E-12	16.128206	24
TGATACT	1140	0.0	15.741228	35
AGCCGTC	260	0.0	15.653846	12
TATACTG	380	0.0	15.578948	5
GACCCTC	1795	0.0	15.45961	7
>>END_MODULE