FastQCFastQC Report
Wed 25 May 2016
SRR2088319_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088319_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1166164
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT164511.4106935216659062No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT164321.4090642482532474No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT139301.1945146651757386No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55260.4738613093870159No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG46710.4005440058173636No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA46420.3980572200822526No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA46020.39462717079244425No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG43710.3748186361438014No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG42400.36358522471967925No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA38660.3315142638599717No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA29140.24987909076253423No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA25240.21643611018690337No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA24680.2116340411811718No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC23180.1987713563443907No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT22000.1886527109394562No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA21110.18102085126963274No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA18100.15520973036382532No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG17150.14706336330053063No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG16710.1432903090817415No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA16700.1432045578494963No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG16450.14106077704336611No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15450.1324856538188454No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC14630.12545405277473837No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG14540.1246822916845315No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT13980.11988022267879989No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC13880.11902271035634782No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT12980.11130509945427916No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT12520.10736054277099961No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA12250.10504525950037903No Hit
ATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGGA11850.10161521021057074No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11820.1013579565138351No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42000.030.4369071
TGCGTCG358.869474E-426.4285710
ACGTTTA751.3737008E-824.66666626
TCACGTT851.9772415E-923.94117524
GTATTGG3150.023.4920641
CGTATTA400.001931204223.12535
ATTGGAC3350.022.6417923
CCCTTCG555.143155E-420.18181814
TTACGTT609.236925E-418.518
GCGCTAG609.236925E-418.511
GTATCAA74050.017.3133012
TTGGACC6200.017.3064524
CGAATTA752.0672992E-417.26666615
GCCGTCA650.001579952717.07692313
TATTGGA4800.016.5729182
TTTTTAC8300.016.4939771
ACTAGCC803.382619E-416.18753
TACGTTA803.382619E-416.187519
CGAACTA700.00259252415.85714224
AATTACG700.00259252415.85714216
Produced by FastQC (version 0.11.5)