##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088319_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1166164
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.886912989939667	31.0	31.0	33.0	28.0	34.0
2	31.20860187760898	31.0	31.0	34.0	28.0	34.0
3	31.262042903056518	31.0	31.0	34.0	28.0	34.0
4	35.163984653959474	35.0	35.0	37.0	33.0	37.0
5	34.89393858839752	35.0	35.0	37.0	32.0	37.0
6	34.98151374935258	36.0	35.0	37.0	32.0	37.0
7	34.792886763782796	35.0	35.0	37.0	32.0	37.0
8	34.86832898288748	35.0	35.0	37.0	32.0	37.0
9	36.325805804329406	38.0	35.0	39.0	32.0	39.0
10	36.11101526028929	38.0	35.0	39.0	31.0	39.0
11	36.23497809913528	38.0	35.0	39.0	32.0	39.0
12	35.972983216768824	38.0	35.0	39.0	30.0	39.0
13	36.071964149124824	38.0	35.0	39.0	31.0	39.0
14	36.82352739408865	38.0	36.0	40.0	31.0	41.0
15	36.95881625568959	38.0	36.0	40.0	31.0	41.0
16	36.939999005285706	38.0	36.0	40.0	31.0	41.0
17	37.05290679527065	38.0	36.0	40.0	31.0	41.0
18	37.06555167197752	39.0	36.0	40.0	31.0	41.0
19	37.16425734287802	39.0	36.0	40.0	31.0	41.0
20	37.15653544441434	39.0	36.0	40.0	31.0	41.0
21	37.07131243975976	39.0	36.0	40.0	31.0	41.0
22	36.990968680219936	39.0	36.0	40.0	31.0	41.0
23	36.88109819888112	39.0	35.0	40.0	31.0	41.0
24	36.789966934324845	39.0	35.0	40.0	30.0	41.0
25	36.665126860372986	38.0	35.0	40.0	30.0	41.0
26	36.42225536030953	38.0	35.0	40.0	30.0	41.0
27	36.29613587797257	38.0	35.0	40.0	30.0	41.0
28	36.109974240329834	38.0	35.0	40.0	30.0	41.0
29	35.88621240237222	38.0	34.0	40.0	29.0	41.0
30	35.63256368744019	38.0	34.0	40.0	28.0	41.0
31	35.37388995029858	38.0	34.0	40.0	27.0	41.0
32	35.24993225652653	37.0	34.0	40.0	27.0	41.0
33	35.05858609938225	37.0	33.0	40.0	27.0	41.0
34	35.179683132046605	38.0	33.0	40.0	27.0	41.0
35	35.08477967078387	38.0	33.0	40.0	26.0	41.0
36	34.997690719315635	38.0	33.0	40.0	25.0	41.0
37	34.86080516977029	38.0	33.0	40.0	25.0	41.0
38	34.794606075989314	38.0	33.0	40.0	25.0	41.0
39	34.66129721034091	38.0	33.0	40.0	24.0	41.0
40	34.435236381846806	38.0	33.0	40.0	23.0	41.0
41	34.278851859601225	38.0	33.0	40.0	23.0	41.0
42	34.08266590290903	38.0	33.0	40.0	21.0	41.0
43	33.6995585526564	37.0	33.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	1.0
14	2.0
15	3.0
16	10.0
17	40.0
18	106.0
19	220.0
20	466.0
21	1010.0
22	1901.0
23	3133.0
24	5163.0
25	8027.0
26	12487.0
27	18086.0
28	24643.0
29	33251.0
30	41675.0
31	51143.0
32	61474.0
33	74196.0
34	88765.0
35	105358.0
36	127609.0
37	159648.0
38	183787.0
39	163959.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.05191379600125	22.752803207782097	14.885556405445547	22.30972659077111
2	18.398441385602712	24.07174291094563	36.01980510459935	21.510010598852304
3	19.572461506271846	25.212577304735866	33.94831258725188	21.266648601740407
4	13.198915418414561	16.585574584706784	36.6111456021623	33.60436439471635
5	11.65788002373594	38.88200973447988	35.64027015068206	13.819840091102108
6	31.07032973063823	37.558353713542864	13.568760483088141	17.802556072730766
7	23.92150675205203	34.78198606713978	21.97675455596297	19.31975262484522
8	26.07712122823205	34.62034499435757	19.566201666317944	19.736332111092437
9	23.645988042848177	14.63284752401892	23.553119458326616	38.168044974806286
10	15.87632614280667	27.760589419669962	34.47962722224318	21.883457215280185
11	31.523610744286394	22.722618774031783	25.055052291101422	20.698718190580397
12	21.122672282800703	27.531204873414033	31.644348479287647	19.701774364497616
13	31.571202678182487	21.436178787889183	27.921115726432987	19.071502807495342
14	19.882709464535004	23.73791336381504	29.19692255977718	27.182454611872775
15	21.17317975859313	30.14747496921531	27.390144096370665	21.289201175820896
16	20.594787697099207	29.10070967719806	28.37877005292566	21.925732572777072
17	20.650697500523084	27.01952727060688	27.929948103354246	24.399827125515795
18	22.613971962777104	25.316936554378287	31.577977025529858	20.49111445731475
19	21.710411228609356	24.744547079141526	33.0775088238018	20.467532868447318
20	22.84378526519426	23.93128239252798	33.20613567216961	20.01879667010815
21	21.67113716424105	24.929769740791173	33.909724532741535	19.48936856222624
22	19.62622752888959	25.306389152812127	33.25578563563958	21.8115976826587
23	21.262446791360393	25.62041016529408	34.447127505222255	18.670015538123284
24	21.00304931381864	25.542205041486447	31.637145375779053	21.817600268915864
25	20.62334285743686	26.395086797397276	32.676707564287696	20.30486278087816
26	20.31961199282434	27.216240597377382	32.461300468887735	20.00284694091054
27	18.850178877070466	27.27609495748454	32.24906616908085	21.624659996364148
28	19.810249673287807	26.50030355936215	32.26861745003276	21.42082931731729
29	20.011936571528533	26.41386631725898	31.175460741370852	22.398736369841636
30	20.30297625376877	26.706192267982892	33.422143026195286	19.568688452053056
31	21.686915390974168	26.204461808116182	32.90677812040159	19.20184468050806
32	20.905635913988082	26.43564713024926	31.470101975365385	21.18861498039727
33	20.331016906712946	26.365159617343704	32.27016097221317	21.03366250373018
34	19.15536751263116	27.260488233215913	33.332790242195784	20.25135401195715
35	18.751221955059492	29.806356567343872	31.60996223515732	19.832459242439313
36	21.63109133878254	26.877008722615344	31.193640002606838	20.29825993599528
37	20.680453178112167	28.06457753797922	30.96991503767909	20.28505424622952
38	20.46213054081587	26.074119935103468	32.46181497628121	21.001934547799454
39	19.99804487190481	25.71550828185401	33.251498074027324	21.03494877221386
40	18.265098219461414	26.54566596121986	32.816653575311875	22.372582244006846
41	20.555685135195393	25.441104338669344	32.74239300818753	21.260817517947732
42	18.80018590867151	28.132149508988448	31.865586658480282	21.202077923859765
43	19.247292833598017	28.398064080180834	30.52177909796564	21.83286398825551
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	73.0
1	127.5
2	182.0
3	489.0
4	796.0
5	796.0
6	1400.0
7	2004.0
8	2321.0
9	2638.0
10	4070.5
11	5503.0
12	5503.0
13	10278.5
14	15054.0
15	25589.5
16	36125.0
17	34594.0
18	33063.0
19	33063.0
20	34905.5
21	36748.0
22	29795.0
23	22842.0
24	25122.5
25	27403.0
26	27403.0
27	29203.5
28	31004.0
29	32124.0
30	33244.0
31	36050.5
32	38857.0
33	38857.0
34	41910.5
35	44964.0
36	48989.0
37	53014.0
38	54975.5
39	56937.0
40	56937.0
41	58304.0
42	59671.0
43	62227.5
44	64784.0
45	70664.0
46	76544.0
47	76544.0
48	107020.0
49	137496.0
50	123906.5
51	110317.0
52	97353.0
53	84389.0
54	84389.0
55	71686.0
56	58983.0
57	47747.5
58	36512.0
59	32077.0
60	27642.0
61	27642.0
62	24112.5
63	20583.0
64	18006.5
65	15430.0
66	13333.0
67	11236.0
68	11236.0
69	9598.5
70	7961.0
71	6864.5
72	5768.0
73	4788.0
74	3808.0
75	3808.0
76	3025.0
77	2242.0
78	1704.0
79	1166.0
80	887.5
81	609.0
82	609.0
83	455.0
84	301.0
85	225.5
86	150.0
87	112.5
88	75.0
89	75.0
90	54.5
91	34.0
92	20.5
93	7.0
94	5.5
95	4.0
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1166164.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.00000252296554
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.45068323989324	36.871823102033524
2	11.885177824083092	11.172067754355988
3	3.86385916143276	5.4480417100716965
4	1.691681508551194	3.1803614067984105
5	0.9847442598452577	2.3141491348601475
6	0.6097751137090729	1.7195659129660812
7	0.4116186322896248	1.3542253729278393
8	0.2848694201945059	1.0711090774286007
9	0.2226799763929734	0.9419363507055291
>10	1.384383498274289	12.232298259946788
>50	0.12665601194590603	4.1048960067941485
>100	0.06937280495975778	6.261150940555893
>500	0.006606933460955927	2.283141601328191
>1k	0.00715751124936892	6.5306182242683395
>5k	1.8352592947099797E-4	0.4766572401276808
>10k+	5.505777884129939E-4	4.037957904831183
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	16451	1.4106935216659062	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	16432	1.4090642482532474	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	13930	1.1945146651757386	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5526	0.4738613093870159	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	4671	0.4005440058173636	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	4642	0.3980572200822526	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	4602	0.39462717079244425	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	4371	0.3748186361438014	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	4240	0.36358522471967925	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3866	0.3315142638599717	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	2914	0.24987909076253423	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	2524	0.21643611018690337	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	2468	0.2116340411811718	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	2318	0.1987713563443907	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2200	0.1886527109394562	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	2111	0.18102085126963274	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	1810	0.15520973036382532	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	1715	0.14706336330053063	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	1671	0.1432903090817415	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1670	0.1432045578494963	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	1645	0.14106077704336611	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1545	0.1324856538188454	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	1463	0.12545405277473837	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG	1454	0.1246822916845315	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	1398	0.11988022267879989	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	1388	0.11902271035634782	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	1298	0.11130509945427916	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	1252	0.10736054277099961	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA	1225	0.10504525950037903	No Hit
ATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGGA	1185	0.10161521021057074	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1182	0.1013579565138351	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	8.575123224520736E-5	0.0
4	0.0	0.0	0.0	8.575123224520736E-5	0.0
5	8.575123224520736E-5	0.0	0.0	8.575123224520736E-5	0.0
6	8.575123224520736E-5	0.0	0.0	8.575123224520736E-5	0.0
7	8.575123224520736E-5	0.0	0.0	8.575123224520736E-5	0.0
8	8.575123224520736E-5	0.0	0.0	1.7150246449041472E-4	0.0
9	8.575123224520736E-5	0.0	0.0	1.7150246449041472E-4	0.0
10	8.575123224520736E-5	0.0	0.0	4.2875616122603683E-4	0.0
11	8.575123224520736E-5	0.0	0.0	0.0012862684836781105	0.0
12	8.575123224520736E-5	0.0	0.0	0.0013720197159233177	0.0
13	8.575123224520736E-5	0.0	0.0	0.0013720197159233177	0.0
14	8.575123224520736E-5	0.0	0.0	0.0015435221804137326	0.0
15	1.7150246449041472E-4	0.0	0.0	0.002401034502865806	0.0
16	2.572536967356221E-4	0.0	0.0	0.0031727955930726725	0.0
17	2.572536967356221E-4	0.0	0.0	0.00454481530899599	0.0
18	2.572536967356221E-4	0.0	0.0	0.005230825166957649	0.0
19	2.572536967356221E-4	0.0	0.0	0.0065170936506357595	0.0
20	3.4300492898082943E-4	0.0	0.0	0.010118645404934469	0.0
21	3.4300492898082943E-4	0.0	0.0	0.015692475500872948	0.0
22	3.4300492898082943E-4	0.0	0.0	0.025210862280090965	0.0
23	3.4300492898082943E-4	0.0	0.0	0.031127697305010273	0.0
24	3.4300492898082943E-4	0.0	0.0	0.03850230327809811	0.0
25	3.4300492898082943E-4	0.0	0.0	0.04433338707077221	0.0
26	3.4300492898082943E-4	0.0	0.0	0.055223793565913545	0.0
27	3.4300492898082943E-4	0.0	0.0	0.13943150363070717	0.0
28	3.4300492898082943E-4	0.0	0.0	0.244391011898841	0.0
29	3.4300492898082943E-4	0.0	0.0	0.3653860005968286	0.0
30	3.4300492898082943E-4	0.0	0.0	0.5709317042885906	0.0
31	4.2875616122603683E-4	0.0	0.0	0.8395903149128253	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4200	0.0	30.436907	1
TGCGTCG	35	8.869474E-4	26.42857	10
ACGTTTA	75	1.3737008E-8	24.666666	26
TCACGTT	85	1.9772415E-9	23.941175	24
GTATTGG	315	0.0	23.492064	1
CGTATTA	40	0.0019312042	23.125	35
ATTGGAC	335	0.0	22.641792	3
CCCTTCG	55	5.143155E-4	20.181818	14
TTACGTT	60	9.236925E-4	18.5	18
GCGCTAG	60	9.236925E-4	18.5	11
GTATCAA	7405	0.0	17.313301	2
TTGGACC	620	0.0	17.306452	4
CGAATTA	75	2.0672992E-4	17.266666	15
GCCGTCA	65	0.0015799527	17.076923	13
TATTGGA	480	0.0	16.572918	2
TTTTTAC	830	0.0	16.493977	1
ACTAGCC	80	3.382619E-4	16.1875	3
TACGTTA	80	3.382619E-4	16.1875	19
CGAACTA	70	0.002592524	15.857142	24
AATTACG	70	0.002592524	15.857142	16
>>END_MODULE