FastQCFastQC Report
Wed 25 May 2016
SRR2088318_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088318_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences764498
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT116331.5216521168139092No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT116091.5185128018647531No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT101581.3287150522303524No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38990.5100078744483308No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG36940.48319289259095516No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA36140.47272850942710115No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA34210.44748318504430357No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG33700.4408121407773467No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA33690.4406813359877985No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG33480.4379344354072868No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA23140.30268228301447486No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA19780.25873187372628836No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA19380.2534996821443614No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC19170.2507527815638498No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA16420.21478146443810187No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA15890.20784881059204863No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT14710.1924138454253641No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA13070.17096185993946356No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG13040.17056944557081902No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC12740.1666453018843738No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG12450.16285196298747676No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT12360.16167471988154317No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG12110.15840460014283883No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG11880.15539608998323085No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10410.13616778591964923No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC10050.13145881349591498No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA9760.12766547459901792No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT9730.12727306023037338No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT9730.12727306023037338No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG9680.12661903628263255No Hit
ATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGGA8980.11746270101426035No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG8910.11654706748742312No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCCT8890.11628545790832677No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT8890.11628545790832677No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC8580.11223050943233337No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA8500.11118407111594798No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA8420.1101376327995626No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT8360.10935280406227355No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8220.10752153700859912No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC7980.10438222205944292No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7950.10398980769079841No Hit
GCCGAGGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAA7830.10242015021622031No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC7800.1020277358475758No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG7730.10111210232073857No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA29650.029.5750431
GGTCTAC400.001930598523.1250021
ATTGGAC2800.023.1253
GTATTGG2750.022.21
CACGGGC500.007032777618.519
TAGAACA609.23296E-418.54
TCCTAGG701.21851954E-418.52
CGTCACA500.007032777618.517
TTGGACC4950.017.9393944
TGGACCC4750.017.9157895
TATTGGA3350.017.6716422
GTCACCC852.7211834E-517.41176411
GGACCCT5000.017.026
TCGAACC1554.0017767E-1016.70967731
GTATCAA53250.016.571832
TAAGGTC803.3807114E-416.18755
TTGCAAC803.3807114E-416.187511
GACTGTT803.3807114E-416.18757
TTAGGTT700.002591428815.8571424
GCCGAGT700.002591428815.85714212
Produced by FastQC (version 0.11.5)