##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088318_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	764498
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.901749906474574	31.0	31.0	33.0	28.0	34.0
2	31.240683428864433	31.0	31.0	34.0	28.0	34.0
3	31.300351341664726	31.0	31.0	34.0	28.0	34.0
4	35.200310530570384	35.0	35.0	37.0	33.0	37.0
5	34.9295917059299	35.0	35.0	37.0	32.0	37.0
6	35.02438332081967	36.0	35.0	37.0	32.0	37.0
7	34.8182205839649	35.0	35.0	37.0	32.0	37.0
8	34.902618450277174	35.0	35.0	37.0	32.0	37.0
9	36.34991196837663	38.0	35.0	39.0	32.0	39.0
10	36.14180024015759	38.0	35.0	39.0	31.0	39.0
11	36.27342909987992	38.0	35.0	39.0	32.0	39.0
12	36.0132295964149	38.0	35.0	39.0	30.0	39.0
13	36.0848530669799	38.0	35.0	39.0	31.0	39.0
14	36.803597916541314	38.0	36.0	40.0	31.0	41.0
15	36.96166111618343	38.0	36.0	40.0	31.0	41.0
16	36.94696519807769	38.0	36.0	40.0	31.0	41.0
17	37.08191649945454	39.0	36.0	40.0	31.0	41.0
18	37.09808658753849	39.0	36.0	40.0	31.0	41.0
19	37.21195869707965	39.0	36.0	40.0	31.0	41.0
20	37.21264411417688	39.0	36.0	40.0	31.0	41.0
21	37.1266870547732	39.0	36.0	40.0	31.0	41.0
22	37.049230998642244	39.0	36.0	40.0	31.0	41.0
23	36.92135100418837	39.0	35.0	40.0	31.0	41.0
24	36.82685893226667	39.0	35.0	40.0	31.0	41.0
25	36.70411171775466	38.0	35.0	40.0	30.0	41.0
26	36.455207207867126	38.0	35.0	40.0	30.0	41.0
27	36.326649644603386	38.0	35.0	40.0	30.0	41.0
28	36.14627638005593	38.0	35.0	40.0	30.0	41.0
29	35.90435292178658	38.0	34.0	40.0	29.0	41.0
30	35.63569819672517	38.0	34.0	40.0	28.0	41.0
31	35.351596734066014	38.0	34.0	40.0	27.0	41.0
32	35.24410135801533	37.0	34.0	40.0	27.0	41.0
33	35.02662400686464	37.0	33.0	40.0	27.0	41.0
34	35.12420830401126	37.0	33.0	40.0	27.0	41.0
35	35.01095359307676	37.0	33.0	40.0	26.0	41.0
36	34.918562246075204	38.0	33.0	40.0	25.0	41.0
37	34.765278653443175	37.0	33.0	40.0	25.0	41.0
38	34.696984164772175	38.0	33.0	40.0	24.0	41.0
39	34.55365220052897	38.0	33.0	40.0	24.0	41.0
40	34.31411462162099	38.0	33.0	40.0	23.0	41.0
41	34.14991536930116	38.0	33.0	40.0	22.0	41.0
42	33.93444848776583	37.0	33.0	40.0	21.0	41.0
43	33.53383527491243	37.0	32.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	0.0
14	2.0
15	1.0
16	9.0
17	30.0
18	63.0
19	150.0
20	349.0
21	650.0
22	1160.0
23	2022.0
24	3388.0
25	5418.0
26	8122.0
27	11700.0
28	16306.0
29	21755.0
30	27561.0
31	33499.0
32	40498.0
33	48572.0
34	58140.0
35	68882.0
36	83903.0
37	106364.0
38	119950.0
39	106003.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.526332312183946	22.348129099095093	15.051837938097941	22.073700650623024
2	18.393638701474693	24.041004685427563	36.312325212099964	21.25303140099778
3	19.581476995361662	25.200719949561673	34.22625042838568	20.99155262669098
4	13.029726696472718	16.182906953321	36.56582489424432	34.221541455961955
5	11.530834613040192	38.916648572004114	35.65607758293678	13.89643923201892
6	31.234090867471203	37.23816151252194	13.146535373539237	18.381212246467616
7	23.645320197044335	35.016311357256654	21.635242996057542	19.703125449641465
8	26.354156583797472	34.416048178020084	19.390894416989973	19.838900821192468
9	23.257091581665353	14.568775850296534	23.786589369756364	38.38754319828175
10	15.913318282062217	27.45827981237361	34.2645762317233	22.36382567384087
11	31.309957645409142	22.431059335668635	25.284304210082958	20.974678808839265
12	21.299336296497835	27.359391391475192	31.69046877820478	19.650803533822195
13	32.04089480940434	21.521835243519277	28.016554654165216	18.420715292911165
14	19.8691167275781	23.766183822586846	29.128395365324693	27.23630408451036
15	20.737529725388427	30.087063667923264	27.738856085954446	21.436550520733867
16	20.546816342227185	29.051612953859916	28.216162763015728	22.185407940897164
17	20.574285348032305	26.524333614999644	27.592747136029132	25.308633900938915
18	23.050158404600143	24.880902239116388	31.828075416809458	20.240863939474007
19	21.720527718842952	24.16082187265369	33.29492032680268	20.823730081700674
20	23.217196120853163	23.389858443056752	33.421800972664414	19.971144463425674
21	21.951790586764126	24.357290666555045	34.28681304594649	19.404105700734338
22	19.329808580270974	24.84793943215025	33.538871259310035	22.283380728268746
23	21.195869707965226	25.483389099775277	35.04260311995584	18.27813807230366
24	20.994691941640134	25.106148086718346	31.791580880525522	22.107579091116
25	20.509798586785053	26.0476809618861	33.031217871073565	20.411302580255278
26	20.31673071741195	27.125512427763056	32.801786270206065	19.755970584618925
27	18.36446923340545	27.43905150831003	32.299234268761985	21.897244989522534
28	19.605021857480335	26.28116751122959	32.35979688632279	21.754013744967285
29	19.995343349492085	26.09555551486073	31.1987735742932	22.71032756135399
30	20.18396385602055	26.402031136772102	33.864182770916344	19.549822236291003
31	21.64348369779908	25.815894874806737	33.56097726874368	18.979644158650512
32	20.827654225387114	26.100002877705368	31.56384974192215	21.508493154985363
33	20.129941477937155	25.993266169434058	32.77680255540237	21.099989797226414
34	18.787099508435603	27.094119278271496	33.868106914602784	20.25067429869012
35	18.30102891047459	30.20125624919882	31.61839533916374	19.879319501162854
36	21.70574677762401	26.811973347216085	31.342789647585732	20.13949022757417
37	20.619543805215972	28.231990142551062	30.947759183150254	20.20070686908272
38	20.268986969226866	25.954286342148702	32.70996130794325	21.06676538068118
39	19.717906390860406	25.42164923910854	33.84639331953779	21.014051050493265
40	17.840596051265013	26.21171016797951	33.20636548427857	22.741328296476905
41	20.486776943824577	25.082080005441483	33.030694651915375	21.400448398818572
42	18.64896965067273	28.13663345097044	32.191712731753384	21.022684166603444
43	19.136217491739675	28.55102302425906	30.414991275320535	21.89776820868073
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	64.0
1	87.5
2	111.0
3	316.0
4	521.0
5	521.0
6	919.5
7	1318.0
8	1550.0
9	1782.0
10	2763.0
11	3744.0
12	3744.0
13	7158.0
14	10572.0
15	17764.0
16	24956.0
17	24074.5
18	23193.0
19	23193.0
20	24005.5
21	24818.0
22	19761.0
23	14704.0
24	15618.5
25	16533.0
26	16533.0
27	17571.5
28	18610.0
29	19067.0
30	19524.0
31	21218.0
32	22912.0
33	22912.0
34	24897.5
35	26883.0
36	29334.5
37	31786.0
38	33329.0
39	34872.0
40	34872.0
41	36398.5
42	37925.0
43	39377.0
44	40829.0
45	45922.5
46	51016.0
47	51016.0
48	75449.0
49	99882.0
50	90230.0
51	80578.0
52	69884.5
53	59191.0
54	59191.0
55	49515.5
56	39840.0
57	31223.5
58	22607.0
59	19460.5
60	16314.0
61	16314.0
62	14241.5
63	12169.0
64	10442.5
65	8716.0
66	7453.5
67	6191.0
68	6191.0
69	5302.0
70	4413.0
71	3753.0
72	3093.0
73	2604.0
74	2115.0
75	2115.0
76	1707.5
77	1300.0
78	990.5
79	681.0
80	533.0
81	385.0
82	385.0
83	278.0
84	171.0
85	141.5
86	112.0
87	76.5
88	41.0
89	41.0
90	30.5
91	20.0
92	11.0
93	2.0
94	2.0
95	2.0
96	2.0
97	2.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	764498.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.39137693559062
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.8162940857361	40.41019420661726
2	10.126705263981357	10.00343833617866
3	3.0493010868791686	4.518275381164655
4	1.395887974218504	2.7577931637793656
5	0.7662079908445999	1.89220338434336
6	0.4868408883480983	1.4427445094435225
7	0.3719177721119576	1.2858671609998753
8	0.2809198780408694	1.110001566801338
9	0.20518693665639984	0.912101879758986
>10	1.3287930243646306	12.283544842457697
>50	0.09431815313160066	3.2144975928987596
>100	0.05987607414743056	5.5761971748654116
>500	0.010862473628480071	3.8923329727094345
>1k	0.0060935827671961375	6.330193563786144
>5k	0.0	0.0
>10k+	7.948151435473224E-4	4.370614264195498
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	11633	1.5216521168139092	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	11609	1.5185128018647531	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	10158	1.3287150522303524	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3899	0.5100078744483308	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	3694	0.48319289259095516	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	3614	0.47272850942710115	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	3421	0.44748318504430357	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	3370	0.4408121407773467	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	3369	0.4406813359877985	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	3348	0.4379344354072868	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	2314	0.30268228301447486	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	1978	0.25873187372628836	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	1938	0.2534996821443614	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	1917	0.2507527815638498	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	1642	0.21478146443810187	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	1589	0.20784881059204863	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1471	0.1924138454253641	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	1307	0.17096185993946356	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	1304	0.17056944557081902	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	1274	0.1666453018843738	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	1245	0.16285196298747676	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	1236	0.16167471988154317	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	1211	0.15840460014283883	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG	1188	0.15539608998323085	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1041	0.13616778591964923	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	1005	0.13145881349591498	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	976	0.12766547459901792	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	973	0.12727306023037338	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	973	0.12727306023037338	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG	968	0.12661903628263255	No Hit
ATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGGA	898	0.11746270101426035	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	891	0.11654706748742312	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCCT	889	0.11628545790832677	No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT	889	0.11628545790832677	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	858	0.11223050943233337	No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA	850	0.11118407111594798	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA	842	0.1101376327995626	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT	836	0.10935280406227355	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	822	0.10752153700859912	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC	798	0.10438222205944292	No Hit
GGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	795	0.10398980769079841	No Hit
GCCGAGGGGAGAAGAGTAAAAAAAAAAAAAAAAAAAAAAAAAA	783	0.10242015021622031	No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC	780	0.1020277358475758	No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG	773	0.10111210232073857	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	1.308047895481741E-4	0.0
6	0.0	0.0	0.0	1.308047895481741E-4	0.0
7	0.0	0.0	0.0	1.308047895481741E-4	0.0
8	0.0	0.0	0.0	1.308047895481741E-4	0.0
9	0.0	0.0	0.0	1.308047895481741E-4	0.0
10	0.0	0.0	0.0	2.616095790963482E-4	0.0
11	0.0	0.0	0.0	9.156335268372187E-4	0.0
12	0.0	0.0	0.0	0.001177243105933567	0.0
13	0.0	0.0	0.0	0.001177243105933567	0.0
14	0.0	0.0	0.0	0.001308047895481741	0.0
15	0.0	0.0	0.0	0.001438852685029915	0.0
16	0.0	0.0	0.0	0.0024852910014153077	0.0
17	0.0	0.0	0.0	0.004054948475993397	0.0
18	0.0	0.0	0.0	0.005493801161023312	0.0
19	0.0	0.0	0.0	0.007848287372890447	0.0
20	0.0	0.0	0.0	0.010725992742950276	0.0
21	0.0	0.0	0.0	0.020928766327707855	0.0
22	0.0	0.0	0.0	0.034140050072073436	0.0
23	0.0	0.0	0.0	0.0434271901299938	0.0
24	0.0	0.0	0.0	0.05336835413565503	0.0
25	0.0	0.0	0.0	0.06095503192944913	0.0
26	0.0	0.0	0.0	0.07377390130517018	0.0
27	0.0	0.0	0.0	0.17684807546913137	0.0
28	0.0	0.0	0.0	0.302028259066734	0.0
29	0.0	0.0	0.0	0.44525950362198463	0.0
30	0.0	0.0	0.0	0.6928729702366782	0.0
31	0.0	0.0	0.0	0.9976481298839238	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2965	0.0	29.575043	1
GGTCTAC	40	0.0019305985	23.125002	1
ATTGGAC	280	0.0	23.125	3
GTATTGG	275	0.0	22.2	1
CACGGGC	50	0.0070327776	18.5	19
TAGAACA	60	9.23296E-4	18.5	4
TCCTAGG	70	1.21851954E-4	18.5	2
CGTCACA	50	0.0070327776	18.5	17
TTGGACC	495	0.0	17.939394	4
TGGACCC	475	0.0	17.915789	5
TATTGGA	335	0.0	17.671642	2
GTCACCC	85	2.7211834E-5	17.411764	11
GGACCCT	500	0.0	17.02	6
TCGAACC	155	4.0017767E-10	16.709677	31
GTATCAA	5325	0.0	16.57183	2
TAAGGTC	80	3.3807114E-4	16.1875	5
TTGCAAC	80	3.3807114E-4	16.1875	11
GACTGTT	80	3.3807114E-4	16.1875	7
TTAGGTT	70	0.0025914288	15.857142	4
GCCGAGT	70	0.0025914288	15.857142	12
>>END_MODULE