FastQCFastQC Report
Wed 25 May 2016
SRR2088317_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088317_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1347669
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT203331.5087532621140651No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT200981.491315745928711No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT178411.323841388352778No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63740.47296480070403046No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG59650.4426161023218609No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA57840.4291855047493116No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG55800.4140482566564936No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG55620.412712617118892No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA55190.40952192266795484No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA53260.3952008987370044No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA41150.30534203873503063No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA32180.2387826684445513No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC32050.2378180398896168No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA30760.2282459565368054No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA28420.2108826425479847No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA25050.18587650231622158No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT24980.1853570869404876No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA22220.16487728069726318No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG21270.15782807202658813No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG20410.15144668312471385No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG20380.1512240765351136No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT20150.14951742601484488No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC20040.14870120185297725No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG19640.14573311399164038No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18200.13504799769082765No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC17680.13118948347108972No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT17570.13037325930922206No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT16780.12451128578308175No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCCT16200.12020755838414328No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA15960.11842670566734115No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG15800.11723947052280641No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT15620.11590383098520482No Hit
ACGCACATGCGAGGGTCCAAAAAAAAAAAAAAAAAAAAAAAAA15360.11397457387533586No Hit
ATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGGA14810.10989345306599767No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA14590.10826100474226238No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA14530.10781579156306186No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT14390.10677696081159395No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG14360.10655435422199369No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13760.10210222242998837No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC13610.10098918948198705No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA13500.1001729653201194No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47800.031.0784511
AATCGAC358.8701915E-426.4285726
TGGACCG400.001931359523.1255
GTATTGG4600.022.1195661
ATTGGAC5300.020.9433963
ATCGACC450.00382614520.5555577
TGCGCTA500.00703549818.4999985
CAATGCG500.00703549818.49999819
TCGACCC500.00703549818.4999988
GGACCCT9150.018.3989076
TTGGACC10400.017.9663474
TATTGGA5500.017.8272722
TGGACCC9550.017.6282735
GTATCAA85400.017.5251752
AAATTCG852.723679E-517.41176626
CTAATAC2250.017.2666663
TATACTG1452.9813236E-916.5862065
GTATTAG1801.0913936E-1116.4444451
TTTTTAC11050.016.407241
CACTTAC1059.345407E-615.8571441
Produced by FastQC (version 0.11.5)