##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088317_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1347669
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.94132758117906	31.0	31.0	33.0	28.0	34.0
2	31.269414819217477	31.0	31.0	34.0	28.0	34.0
3	31.321547056435964	31.0	31.0	34.0	28.0	34.0
4	35.214594236418584	35.0	35.0	37.0	33.0	37.0
5	34.94955363668675	35.0	35.0	37.0	32.0	37.0
6	35.04277905034545	36.0	35.0	37.0	32.0	37.0
7	34.83877346737218	35.0	35.0	37.0	32.0	37.0
8	34.91417180331372	36.0	35.0	37.0	32.0	37.0
9	36.37131224358504	38.0	35.0	39.0	32.0	39.0
10	36.16503829946374	38.0	35.0	39.0	31.0	39.0
11	36.28142667079231	38.0	35.0	39.0	32.0	39.0
12	36.03729847610949	38.0	35.0	39.0	30.0	39.0
13	36.10589840680464	38.0	35.0	39.0	31.0	39.0
14	36.85553425952515	39.0	36.0	40.0	31.0	41.0
15	37.00315804548446	38.0	36.0	40.0	31.0	41.0
16	36.995007676217234	38.0	36.0	40.0	31.0	41.0
17	37.11806088883843	39.0	36.0	40.0	31.0	41.0
18	37.13485952411163	39.0	36.0	40.0	31.0	41.0
19	37.234307533971624	39.0	36.0	40.0	31.0	41.0
20	37.23502952134389	39.0	36.0	40.0	31.0	41.0
21	37.15311697456868	39.0	36.0	40.0	31.0	41.0
22	37.08290463014286	39.0	36.0	40.0	31.0	41.0
23	36.95725285659906	39.0	35.0	40.0	31.0	41.0
24	36.86407196425829	39.0	35.0	40.0	31.0	41.0
25	36.74398387141056	38.0	35.0	40.0	30.0	41.0
26	36.4980970846699	38.0	35.0	40.0	30.0	41.0
27	36.37505351833425	38.0	35.0	40.0	30.0	41.0
28	36.18567689840755	38.0	35.0	40.0	30.0	41.0
29	35.9695214477739	38.0	34.0	40.0	29.0	41.0
30	35.70428272817732	38.0	34.0	40.0	29.0	41.0
31	35.429145435563186	38.0	34.0	40.0	27.0	41.0
32	35.310250514035715	37.0	34.0	40.0	27.0	41.0
33	35.11503047113201	37.0	33.0	40.0	27.0	41.0
34	35.214804228634776	38.0	33.0	40.0	27.0	41.0
35	35.100822234539784	38.0	33.0	40.0	26.0	41.0
36	35.00914467870078	38.0	33.0	40.0	25.0	41.0
37	34.8640437674236	38.0	33.0	40.0	25.0	41.0
38	34.79007604983123	38.0	33.0	40.0	24.0	41.0
39	34.65932435931968	38.0	33.0	40.0	24.0	41.0
40	34.43606775847778	38.0	33.0	40.0	23.0	41.0
41	34.28020233454951	38.0	33.0	40.0	22.0	41.0
42	34.07319082059467	38.0	33.0	40.0	21.0	41.0
43	33.68654840320583	37.0	33.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	4.0
16	15.0
17	45.0
18	101.0
19	264.0
20	562.0
21	1094.0
22	2097.0
23	3586.0
24	6010.0
25	9528.0
26	14056.0
27	20381.0
28	27976.0
29	37757.0
30	48186.0
31	57804.0
32	69920.0
33	84300.0
34	101056.0
35	120058.0
36	146278.0
37	185324.0
38	212933.0
39	198334.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.15192157718253	22.68056919020917	15.008433079636024	22.15907615297228
2	18.12477692964667	24.013685853128624	36.41643459929701	21.4451026179277
3	19.21332315279197	25.3681727486497	34.4211375345133	20.997366564045027
4	12.78800655056991	16.42717907735505	37.12165227515065	33.663162096924395
5	11.275765785218773	38.97655878409313	36.06219331304645	13.685482117641644
6	30.74389928090651	37.82063696649548	13.461391484110713	17.974072268487294
7	23.60527696340867	35.15240018135017	21.966150441985384	19.276172413255775
8	26.198050114679493	34.43197105520718	19.725615117658712	19.644363712454616
9	23.412128645832173	14.517362942977838	23.97287464503524	38.09763376615475
10	15.752903717455844	27.765348909858428	34.49140701463045	21.99034035805528
11	31.162028658372343	22.573421218414907	25.57126416056168	20.693285962651068
12	21.124178117920646	27.64113443286148	31.685673559308704	19.54901388990917
13	31.658886566360138	21.699616152037333	28.130720525589	18.51077675601353
14	19.68176161950746	23.846805113124958	29.529580334637068	26.941852932730516
15	20.76080996149648	30.130618126557785	28.012071213332057	21.096500698613678
16	20.345203458712785	29.201235614976675	28.527999085828938	21.925561840481603
17	20.449086533859575	26.789738429837	27.94944455945785	24.811730476845575
18	22.600727626739207	25.26740616575732	32.06974412856569	20.06212207893778
19	21.53229019885447	24.437454597530998	33.4988784337994	20.53137676981514
20	22.87208505946193	23.617075112657485	33.74485871530769	19.765981112572895
21	21.507358260819238	24.7476197790407	34.47983147197123	19.26519048816883
22	19.11945737417719	25.267554570150384	33.75754729091491	21.85544076475752
23	21.090119309711806	25.58276550102436	35.16249168007871	18.16462350918512
24	20.82870497132456	25.314821369342177	31.986118253072526	21.870355406260735
25	20.246959750502537	26.250882078611294	33.341124563969345	20.16103360691683
26	20.06100904598978	27.28459287851839	33.080749056333566	19.573649019158264
27	18.225766119128657	27.43678158360844	32.581368273663635	21.756084023599268
28	19.514658272914197	26.44922454994513	32.741570815979294	21.294546361161384
29	19.71366856401683	26.452192637806466	31.480059272714588	22.35407952546211
30	20.07354921720393	26.429931978846437	34.173376400288205	19.323142403661432
31	21.474635092148	26.14113702993836	33.55171039773119	18.83251748018245
32	20.50948712183778	26.346454507746337	31.91199025873564	21.232068111680242
33	19.977679979282748	26.14113702993836	33.00951494766148	20.87166804311741
34	18.733902760989533	27.362356780485413	33.97206584109303	19.931674617432023
35	18.3710540199411	30.156588895344484	31.848547380699564	19.623809704014857
36	21.694867211459194	26.955951350071867	31.36816236034219	19.98101907812675
37	20.432984657211822	28.388869967328773	31.17456882958649	20.00357654587291
38	20.20177061281368	25.917640013979693	32.891310848583736	20.989278524622886
39	19.627445611644994	25.681009209234613	33.768084002822654	20.923461176297742
40	17.817950846980974	26.483209155957432	33.235905849284954	22.462934147776643
41	20.305134272584738	25.290779857665346	33.15910657587286	21.244979293877055
42	18.520497243759408	28.175909663277853	32.26630574718273	21.03728734578001
43	19.039838417296828	28.44415060374617	30.791017675705234	21.724993303251765
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	123.0
1	153.0
2	183.0
3	524.0
4	865.0
5	865.0
6	1569.5
7	2274.0
8	2592.5
9	2911.0
10	4638.0
11	6365.0
12	6365.0
13	12010.0
14	17655.0
15	30225.5
16	42796.0
17	41725.0
18	40654.0
19	40654.0
20	42500.5
21	44347.0
22	36302.5
23	28258.0
24	30310.5
25	32363.0
26	32363.0
27	34595.5
28	36828.0
29	37242.0
30	37656.0
31	40920.0
32	44184.0
33	44184.0
34	48032.0
35	51880.0
36	55611.0
37	59342.0
38	62299.5
39	65257.0
40	65257.0
41	66976.5
42	68696.0
43	71008.0
44	73320.0
45	80503.0
46	87686.0
47	87686.0
48	127990.5
49	168295.0
50	151724.5
51	135154.0
52	117511.0
53	99868.0
54	99868.0
55	84132.0
56	68396.0
57	53493.5
58	38591.0
59	33061.5
60	27532.0
61	27532.0
62	23839.0
63	20146.0
64	17404.0
65	14662.0
66	12482.5
67	10303.0
68	10303.0
69	8990.0
70	7677.0
71	6612.0
72	5547.0
73	4536.5
74	3526.0
75	3526.0
76	2824.5
77	2123.0
78	1611.0
79	1099.0
80	824.5
81	550.0
82	550.0
83	421.5
84	293.0
85	216.5
86	140.0
87	111.0
88	82.0
89	82.0
90	57.5
91	33.0
92	20.0
93	7.0
94	4.5
95	2.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1347669.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.43526102059989
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.7632409314803	31.807049247830836
2	12.189872971350004	10.10181136751686
3	4.212598647627816	5.23650373618454
4	1.947154605399171	3.2272343728871133
5	1.0942477258009053	2.267022006987917
6	0.6854438771470766	1.7040927578736673
7	0.4772593144935708	1.3842754989387573
8	0.3336723799357615	1.1060641726402445
9	0.2625469240187889	0.9790830294186712
>10	1.7398744149745748	13.872494070838872
>50	0.17708889833831296	5.050011357967399
>100	0.09891595616202432	7.574257966963957
>500	0.007775841906818562	2.284655452348555
>1k	0.008499176037685406	6.033795205512187
>5k	0.0012658347290169752	3.0053960248499734
>10k+	5.425005981501323E-4	4.366253731240531
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	20333	1.5087532621140651	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	20098	1.491315745928711	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	17841	1.323841388352778	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6374	0.47296480070403046	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	5965	0.4426161023218609	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	5784	0.4291855047493116	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	5580	0.4140482566564936	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	5562	0.412712617118892	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	5519	0.40952192266795484	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5326	0.3952008987370044	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	4115	0.30534203873503063	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3218	0.2387826684445513	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	3205	0.2378180398896168	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3076	0.2282459565368054	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	2842	0.2108826425479847	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	2505	0.18587650231622158	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2498	0.1853570869404876	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2222	0.16487728069726318	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	2127	0.15782807202658813	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG	2041	0.15144668312471385	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	2038	0.1512240765351136	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	2015	0.14951742601484488	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	2004	0.14870120185297725	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	1964	0.14573311399164038	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1820	0.13504799769082765	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	1768	0.13118948347108972	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	1757	0.13037325930922206	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	1678	0.12451128578308175	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCCT	1620	0.12020755838414328	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	1596	0.11842670566734115	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG	1580	0.11723947052280641	No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT	1562	0.11590383098520482	No Hit
ACGCACATGCGAGGGTCCAAAAAAAAAAAAAAAAAAAAAAAAA	1536	0.11397457387533586	No Hit
ATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGGA	1481	0.10989345306599767	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA	1459	0.10826100474226238	No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA	1453	0.10781579156306186	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT	1439	0.10677696081159395	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	1436	0.10655435422199369	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1376	0.10210222242998837	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	1361	0.10098918948198705	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA	1350	0.1001729653201194	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	7.420219653342179E-5	0.0	0.0	0.0
3	0.0	7.420219653342179E-5	0.0	0.0	0.0
4	0.0	7.420219653342179E-5	0.0	0.0	0.0
5	0.0	7.420219653342179E-5	0.0	0.0	0.0
6	7.420219653342179E-5	7.420219653342179E-5	0.0	0.0	0.0
7	7.420219653342179E-5	7.420219653342179E-5	0.0	0.0	0.0
8	7.420219653342179E-5	7.420219653342179E-5	0.0	0.0	0.0
9	7.420219653342179E-5	7.420219653342179E-5	0.0	0.0	0.0
10	7.420219653342179E-5	7.420219653342179E-5	0.0	7.420219653342179E-5	0.0
11	7.420219653342179E-5	7.420219653342179E-5	0.0	1.4840439306684357E-4	0.0
12	7.420219653342179E-5	7.420219653342179E-5	0.0	2.9680878613368714E-4	0.0
13	7.420219653342179E-5	7.420219653342179E-5	0.0	4.452131792005307E-4	0.0
14	7.420219653342179E-5	7.420219653342179E-5	0.0	5.936175722673743E-4	0.0
15	7.420219653342179E-5	7.420219653342179E-5	0.0	8.904263584010614E-4	0.0
16	1.4840439306684357E-4	7.420219653342179E-5	0.0	0.0019292571098689663	0.0
17	1.4840439306684357E-4	7.420219653342179E-5	0.0	0.003042290057870293	0.0
18	1.4840439306684357E-4	7.420219653342179E-5	0.0	0.003784312023204511	0.0
19	1.4840439306684357E-4	7.420219653342179E-5	0.0	0.004600536185072151	0.0
20	1.4840439306684357E-4	7.420219653342179E-5	0.0	0.007123410867208491	0.0
21	1.4840439306684357E-4	7.420219653342179E-5	0.0	0.012688575607215124	0.0
22	1.4840439306684357E-4	7.420219653342179E-5	0.0	0.020479806243224413	0.0
23	1.4840439306684357E-4	7.420219653342179E-5	0.0	0.02619337537629789	0.0
24	1.4840439306684357E-4	7.420219653342179E-5	0.0	0.03279737086777243	0.0
25	1.4840439306684357E-4	7.420219653342179E-5	0.0	0.03888195098351301	0.0
26	1.4840439306684357E-4	7.420219653342179E-5	0.0	0.0497154716773926	0.0
27	1.4840439306684357E-4	7.420219653342179E-5	0.0	0.13883230971403215	0.0
28	1.4840439306684357E-4	7.420219653342179E-5	0.0	0.2482805496008293	0.0
29	1.4840439306684357E-4	7.420219653342179E-5	0.0	0.37516630567298054	0.0
30	1.4840439306684357E-4	7.420219653342179E-5	0.0	0.5933207634812405	0.0
31	1.4840439306684357E-4	7.420219653342179E-5	0.0	0.8622295237183611	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4780	0.0	31.078451	1
AATCGAC	35	8.8701915E-4	26.428572	6
TGGACCG	40	0.0019313595	23.125	5
GTATTGG	460	0.0	22.119566	1
ATTGGAC	530	0.0	20.943396	3
ATCGACC	45	0.003826145	20.555557	7
TGCGCTA	50	0.007035498	18.499998	5
CAATGCG	50	0.007035498	18.499998	19
TCGACCC	50	0.007035498	18.499998	8
GGACCCT	915	0.0	18.398907	6
TTGGACC	1040	0.0	17.966347	4
TATTGGA	550	0.0	17.827272	2
TGGACCC	955	0.0	17.628273	5
GTATCAA	8540	0.0	17.525175	2
AAATTCG	85	2.723679E-5	17.411766	26
CTAATAC	225	0.0	17.266666	3
TATACTG	145	2.9813236E-9	16.586206	5
GTATTAG	180	1.0913936E-11	16.444445	1
TTTTTAC	1105	0.0	16.40724	1
CACTTAC	105	9.345407E-6	15.857144	1
>>END_MODULE