FastQCFastQC Report
Wed 25 May 2016
SRR2088316_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088316_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1764642
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT235021.3318282121812808No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT229951.3030971721176308No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT207931.178312654918108No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG76710.43470573634765575No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76560.4338557055765419No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA74150.420198544520645No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG71720.4064280460285996No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA70950.4020645547368815No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA69370.39311089728114823No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG68740.3895407680424698No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA43840.24843566003756004No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA40840.23143504461528175No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA38970.22083799433539494No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC36350.2059907901999386No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA34300.19437370299471507No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA32950.18672342605468983No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG28600.16207253369238633No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT28520.16161918394779223No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC26310.14909539725338058No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG26090.14784868545574684No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG25400.14393854390862282No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA24690.1399150649253503No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT24430.1384416782554195No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC23110.13096140746961707No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG22620.12818464028397827No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT19510.11056066896288314No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19380.10982397562791774No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT19170.10863393254835825No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA19120.10835058895798694No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC18880.10699053972420469No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCCT18730.10614050895309077No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA18570.1052338094639026No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC18210.1031937356132292No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG18090.10251371099633806No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT18030.1021736986878925No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56300.030.2309061
GTATTGG7200.022.611111
ATTGGAC8200.019.4024373
TTGGACC12500.018.6484
TGCGGTA500.00703633918.49999836
GGACCCT12750.017.7019626
TGGACCC13200.017.518945
GTATCAA101750.016.8181822
TCACGTA2450.016.61224625
ACGTTTA1251.659082E-716.27999926
TATTGGA9550.016.2722532
GATACCT7850.016.26114736
ATAGTCG803.3838488E-416.187530
TAAGCCG1151.243041E-616.08695636
GACCCTC14000.015.4607147
TCGTTTG1802.0190782E-1015.41666612
CGATTAA1802.0190782E-1015.41666624
ATACCCT6750.015.07407437
ATCCGTA750.004105563314.812
TACGACT750.004105563314.84
Produced by FastQC (version 0.11.5)