##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088316_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1764642
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.97112275464372	31.0	31.0	33.0	28.0	34.0
2	31.29566336968065	31.0	31.0	34.0	28.0	34.0
3	31.355722577157294	31.0	31.0	34.0	28.0	34.0
4	35.23940833324833	35.0	35.0	37.0	33.0	37.0
5	34.97732967933439	35.0	35.0	37.0	32.0	37.0
6	35.07075599470034	36.0	35.0	37.0	32.0	37.0
7	34.87897828568061	36.0	35.0	37.0	32.0	37.0
8	34.94738706207831	36.0	35.0	37.0	32.0	37.0
9	36.41722230344738	38.0	35.0	39.0	32.0	39.0
10	36.20805239816348	38.0	35.0	39.0	32.0	39.0
11	36.32614207300971	38.0	35.0	39.0	32.0	39.0
12	36.06680165155312	38.0	35.0	39.0	30.0	39.0
13	36.151022700355085	38.0	35.0	39.0	31.0	39.0
14	36.92150079166199	39.0	36.0	40.0	31.0	41.0
15	37.07638319840512	39.0	36.0	40.0	31.0	41.0
16	37.061028242555714	39.0	36.0	40.0	31.0	41.0
17	37.16721975335507	39.0	36.0	40.0	31.0	41.0
18	37.1808083452621	39.0	36.0	40.0	31.0	41.0
19	37.27647364167916	39.0	36.0	40.0	31.0	41.0
20	37.266021663317545	39.0	36.0	40.0	31.0	41.0
21	37.18950189330187	39.0	36.0	40.0	31.0	41.0
22	37.106753097795476	39.0	36.0	40.0	31.0	41.0
23	36.993535799329266	39.0	36.0	40.0	31.0	41.0
24	36.90817570929401	39.0	35.0	40.0	31.0	41.0
25	36.78091703586337	38.0	35.0	40.0	30.0	41.0
26	36.55266223970641	38.0	35.0	40.0	30.0	41.0
27	36.432262181224296	38.0	35.0	40.0	30.0	41.0
28	36.25245630558493	38.0	35.0	40.0	30.0	41.0
29	36.0420748231086	38.0	35.0	40.0	30.0	41.0
30	35.788138330607566	38.0	34.0	40.0	29.0	41.0
31	35.544202166785105	38.0	34.0	40.0	27.0	41.0
32	35.42944517924882	38.0	34.0	40.0	27.0	41.0
33	35.241344136657744	38.0	34.0	40.0	27.0	41.0
34	35.36655310255565	38.0	34.0	40.0	27.0	41.0
35	35.28200054175294	38.0	34.0	40.0	27.0	41.0
36	35.20511582519287	38.0	34.0	40.0	26.0	41.0
37	35.07857571110741	38.0	33.0	40.0	26.0	41.0
38	35.02151654556562	38.0	33.0	40.0	25.0	41.0
39	34.90853215553069	38.0	33.0	40.0	25.0	41.0
40	34.70878059119073	38.0	33.0	40.0	24.0	41.0
41	34.568623550839206	38.0	33.0	40.0	23.0	41.0
42	34.380477173273675	38.0	33.0	40.0	23.0	41.0
43	34.01010743255573	37.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	1.0
15	5.0
16	16.0
17	46.0
18	138.0
19	300.0
20	665.0
21	1266.0
22	2419.0
23	4172.0
24	7109.0
25	11176.0
26	17173.0
27	25064.0
28	34860.0
29	46994.0
30	60918.0
31	73748.0
32	89683.0
33	109410.0
34	131411.0
35	157154.0
36	192688.0
37	245224.0
38	288542.0
39	264459.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.7354012881933	22.257885735463624	14.555303568655853	22.451409407687226
2	18.479215614271904	23.63448223492357	36.26299272033648	21.623309430468048
3	19.55643127614553	25.12866632438761	33.77670938354635	21.53819301592051
4	13.08968051310124	16.09754273104686	36.858240934988515	33.95453582086338
5	11.630347685252874	38.691530633408924	36.0097402192626	13.668381462075594
6	31.463945661499615	37.30105029802079	13.390704743511714	17.844299296967883
7	24.41486715152422	34.46092748557498	21.731886694298332	19.39231866860247
8	26.17613090927225	34.67910205016088	19.455617626691417	19.689149413875448
9	23.55293594961471	14.555360237373927	23.477736560730165	38.4139672522812
10	15.88815181776247	27.285647740448205	34.50031224463659	22.32588819715274
11	31.722978371817064	22.312854391995657	25.115745856666678	20.848421379520605
12	21.218298102391305	27.1286753913825	31.598703873080204	20.054322633145986
13	31.652935836277273	21.336962398038807	27.811590112895416	19.198511652788497
14	19.977026501692695	23.42384460984154	28.759034410378987	27.840094478086776
15	21.231445244984535	29.704721977602254	27.471237792141405	21.592594985271802
16	20.92067399506529	29.135484704546304	27.769485255366245	22.174356045022165
17	20.715929916662983	26.67730905192101	27.447833611576737	25.158927419839266
18	22.859254171667683	25.25883436980419	31.321310498106698	20.560600960421436
19	21.967968573795705	24.349018101121928	32.50098320225859	21.182030122823782
20	23.341391625043492	23.618955006171223	32.88706717849853	20.152586190286755
21	22.017837045701054	24.745132440461013	33.39311883090168	19.843911682936255
22	19.817900741340168	25.13949004953979	32.716097656068484	22.326511553051553
23	21.399014644330126	25.625707650616953	34.20642827270347	18.76884943234945
24	21.524422517428466	25.317033143266453	30.87204090121396	22.286503438091128
25	21.01406404245167	26.17851099543137	32.18749185387178	20.619933108245185
26	20.79050594964871	27.00921773368196	32.00167512730627	20.198601189363057
27	19.18978467020506	27.13915910422624	31.616497850555525	22.054558375013176
28	20.055456007507473	26.096001341915244	31.92046885430586	21.928073796271423
29	20.43060292115908	26.110395196306108	30.73008576243793	22.72891612009688
30	20.66691147552875	26.345400370160064	33.07390394198937	19.91378421232182
31	22.018857082626393	25.93619555694583	32.6689492826307	19.37599807779708
32	21.14451543145862	26.110621871178402	31.096335687351882	21.648527010011094
33	20.607749333859218	25.980680500634122	32.119999410645335	21.291570754861326
34	19.332929852060644	27.181830648936156	32.977170440236605	20.508069058766594
35	18.990310782583663	29.830583200445187	30.919699293114412	20.25940672385674
36	22.038691133952383	26.831504633801078	30.650806225852044	20.478998006394498
37	21.002843636272967	27.971225891710617	30.469522996732483	20.55640747528394
38	20.755201338288444	25.826824931062504	32.17768816564493	21.24028556500412
39	20.12855865382327	25.48828600928687	32.9947944115577	21.388360925332165
40	18.421810202862677	26.352597297355494	32.46890870782856	22.756683791953268
41	20.858168399029378	25.190435227088553	32.32519683879223	21.62619953508984
42	18.997904390805616	27.99077659944623	31.57773644739273	21.433582562355426
43	19.629137241434808	28.04852202316391	30.183855988920133	22.138484746481154
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	88.0
1	146.5
2	205.0
3	674.5
4	1144.0
5	1144.0
6	1989.0
7	2834.0
8	3204.5
9	3575.0
10	5661.0
11	7747.0
12	7747.0
13	14441.5
14	21136.0
15	35202.5
16	49269.0
17	48333.0
18	47397.0
19	47397.0
20	49637.5
21	51878.0
22	41985.0
23	32092.0
24	34654.5
25	37217.0
26	37217.0
27	40089.0
28	42961.0
29	44555.5
30	46150.0
31	50164.0
32	54178.0
33	54178.0
34	60053.0
35	65928.0
36	71426.0
37	76924.0
38	81694.0
39	86464.0
40	86464.0
41	88966.0
42	91468.0
43	95721.5
44	99975.0
45	109081.0
46	118187.0
47	118187.0
48	168290.0
49	218393.0
50	198368.0
51	178343.0
52	156536.5
53	134730.0
54	134730.0
55	114664.5
56	94599.0
57	76227.0
58	57855.0
59	50626.5
60	43398.0
61	43398.0
62	37496.0
63	31594.0
64	27607.0
65	23620.0
66	19834.5
67	16049.0
68	16049.0
69	13691.5
70	11334.0
71	9382.5
72	7431.0
73	6029.5
74	4628.0
75	4628.0
76	3719.5
77	2811.0
78	2140.0
79	1469.0
80	1159.0
81	849.0
82	849.0
83	604.0
84	359.0
85	274.5
86	190.0
87	143.0
88	96.0
89	96.0
90	76.0
91	56.0
92	34.5
93	13.0
94	9.5
95	6.0
96	6.0
97	4.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1764642.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.58990692386104
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.16010633235567	35.633119728511765
2	12.347459494287188	11.258390581813945
3	4.00649122880224	5.479666866370793
4	1.6678758789627368	3.041532243298564
5	0.8815800201858612	2.0095575533104473
6	0.5440238954134894	1.4881199253754378
7	0.3696992975059871	1.1798189594180335
8	0.26522037427158385	0.9673097741882477
9	0.20669112507475962	0.848072623873177
>10	1.3132189361264532	11.546167715575194
>50	0.1375431879014611	4.3581106242212915
>100	0.08604265682403917	7.452981430236735
>500	0.005518689113351407	1.7969709846382187
>1k	0.007274635649417762	6.1865297769356
>5k	8.779732680331783E-4	2.905939840874253
>10k+	3.76274257728505E-4	3.8477113713582938
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	23502	1.3318282121812808	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	22995	1.3030971721176308	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	20793	1.178312654918108	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	7671	0.43470573634765575	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7656	0.4338557055765419	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	7415	0.420198544520645	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	7172	0.4064280460285996	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	7095	0.4020645547368815	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	6937	0.39311089728114823	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	6874	0.3895407680424698	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	4384	0.24843566003756004	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4084	0.23143504461528175	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3897	0.22083799433539494	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	3635	0.2059907901999386	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3430	0.19437370299471507	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3295	0.18672342605468983	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	2860	0.16207253369238633	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2852	0.16161918394779223	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	2631	0.14909539725338058	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	2609	0.14784868545574684	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	2540	0.14393854390862282	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2469	0.1399150649253503	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	2443	0.1384416782554195	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	2311	0.13096140746961707	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG	2262	0.12818464028397827	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	1951	0.11056066896288314	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1938	0.10982397562791774	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	1917	0.10863393254835825	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	1912	0.10835058895798694	No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC	1888	0.10699053972420469	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCCT	1873	0.10614050895309077	No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA	1857	0.1052338094639026	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	1821	0.1031937356132292	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG	1809	0.10251371099633806	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT	1803	0.1021736986878925	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	5.6668718074260955E-5	0.0
5	0.0	0.0	0.0	5.6668718074260955E-5	0.0
6	5.6668718074260955E-5	0.0	0.0	5.6668718074260955E-5	0.0
7	5.6668718074260955E-5	0.0	0.0	5.6668718074260955E-5	5.6668718074260955E-5
8	1.7000615422278287E-4	0.0	0.0	5.6668718074260955E-5	5.6668718074260955E-5
9	1.7000615422278287E-4	0.0	0.0	1.1333743614852191E-4	5.6668718074260955E-5
10	1.7000615422278287E-4	0.0	0.0	1.1333743614852191E-4	5.6668718074260955E-5
11	1.7000615422278287E-4	0.0	0.0	6.233558988168705E-4	5.6668718074260955E-5
12	1.7000615422278287E-4	0.0	0.0	0.001076705643410958	5.6668718074260955E-5
13	1.7000615422278287E-4	0.0	0.0	0.001246711797633741	5.6668718074260955E-5
14	1.7000615422278287E-4	0.0	0.0	0.0015867241060793067	5.6668718074260955E-5
15	1.7000615422278287E-4	0.0	0.0	0.0020967425687476553	5.6668718074260955E-5
16	1.7000615422278287E-4	0.0	0.0	0.0029467733398615696	5.6668718074260955E-5
17	1.7000615422278287E-4	0.0	0.0	0.003910141547124006	5.6668718074260955E-5
18	1.7000615422278287E-4	0.0	0.0	0.004533497445940876	5.6668718074260955E-5
19	1.7000615422278287E-4	0.0	0.0	0.006290227706242966	5.6668718074260955E-5
20	1.7000615422278287E-4	0.0	0.0	0.010540381561812537	5.6668718074260955E-5
21	1.7000615422278287E-4	0.0	0.0	0.018644008246431854	5.6668718074260955E-5
22	1.7000615422278287E-4	0.0	0.0	0.03088445135047222	5.6668718074260955E-5
23	1.7000615422278287E-4	0.0	0.0	0.038251384700126144	5.6668718074260955E-5
24	1.7000615422278287E-4	0.0	0.0	0.04720504215585938	5.6668718074260955E-5
25	1.7000615422278287E-4	0.0	0.0	0.05270190780906269	5.6668718074260955E-5
26	1.7000615422278287E-4	0.0	0.0	0.06324228937087523	5.6668718074260955E-5
27	1.7000615422278287E-4	0.0	0.0	0.15124880854020248	5.6668718074260955E-5
28	1.7000615422278287E-4	0.0	0.0	0.2552925749245456	5.6668718074260955E-5
29	1.7000615422278287E-4	0.0	0.0	0.37554359467812737	1.1333743614852191E-4
30	1.7000615422278287E-4	0.0	0.0	0.5769442187140508	1.1333743614852191E-4
31	1.7000615422278287E-4	0.0	0.0	0.8434005310992257	1.1333743614852191E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5630	0.0	30.230906	1
GTATTGG	720	0.0	22.61111	1
ATTGGAC	820	0.0	19.402437	3
TTGGACC	1250	0.0	18.648	4
TGCGGTA	50	0.007036339	18.499998	36
GGACCCT	1275	0.0	17.701962	6
TGGACCC	1320	0.0	17.51894	5
GTATCAA	10175	0.0	16.818182	2
TCACGTA	245	0.0	16.612246	25
ACGTTTA	125	1.659082E-7	16.279999	26
TATTGGA	955	0.0	16.272253	2
GATACCT	785	0.0	16.261147	36
ATAGTCG	80	3.3838488E-4	16.1875	30
TAAGCCG	115	1.243041E-6	16.086956	36
GACCCTC	1400	0.0	15.460714	7
TCGTTTG	180	2.0190782E-10	15.416666	12
CGATTAA	180	2.0190782E-10	15.416666	24
ATACCCT	675	0.0	15.074074	37
ATCCGTA	75	0.0041055633	14.8	12
TACGACT	75	0.0041055633	14.8	4
>>END_MODULE