FastQCFastQC Report
Wed 25 May 2016
SRR2088315_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088315_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1477575
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT230301.5586349254690963No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT229191.5511226164492495No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT207541.4045987513324196No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA78540.5315466219988834No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77170.5222746730284419No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG76630.5186200362079759No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG73830.4996700675092635No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA71350.48288580951897536No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG70000.47374921746781046No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA69840.4726663621135983No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA47050.31842715259800686No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA43660.2954841547806372No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA40770.27592507994518045No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC38840.26286313723499655No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA34430.2330169365345245No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA31490.21311946940087645No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG29370.1987716359575656No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT28840.1951846775967379No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG27560.1865218347630408No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG26940.18232577026546876No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA26130.1768438150347698No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC25650.1735952489721334No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT24400.16513544151735107No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC23610.15978884320592862No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG22780.15417153105595316No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT20830.14097423142649274No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT19660.13305585164881648No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA19630.1328528162699017No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19210.13001032096509485No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCCT18890.12784461025667054No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC18750.12689711182173494No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC18190.12310711808199246No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG18160.12290408270307768No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA17790.12039997969646211No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT17740.12006158739827082No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT17560.11884337512478216No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA17410.11782819823020828No Hit
ATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGGA17370.11755748439165525No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16850.1140382044904658No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG16640.11261695683806236No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG16370.11078963842782938No Hit
TTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCC16020.10842089234049032No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA15920.10774410774410774No Hit
ACGCACATGCGAGGGTCCAAAAAAAAAAAAAAAAAAAAAAAAA15600.10557839703568346No Hit
TTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAAC15410.10429250630255656No Hit
CTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTG14870.1006378694820906No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57350.029.9032271
GTATTGG5850.025.6153851
CGAATTA551.903136E-523.54545415
ATTGGAC6550.022.595423
TTGGACC10100.021.0643584
TGGACCC10900.019.5183495
TATAGAG1052.26064E-819.380955
GGACCCT10900.019.0091746
TATTGGA8200.018.7256092
ACGTTTA609.238516E-418.526
GACCCTC11450.018.257647
ATAGAGC752.06777E-417.2666666
CCCCGAA752.06777E-417.26666612
CCGAATT752.06777E-417.26666614
ACTTACC1107.8113044E-716.8181828
GTATCAA103400.016.6392632
ATACCCT6100.016.3770537
AAGACGT803.3833826E-416.18755
GTAGTGT803.3833826E-416.18756
GATACCT7950.016.05660236
Produced by FastQC (version 0.11.5)