##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088315_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1477575
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.015777202510872	31.0	31.0	33.0	30.0	34.0
2	31.325848772481937	31.0	31.0	34.0	30.0	34.0
3	31.37621305179094	31.0	31.0	34.0	28.0	34.0
4	35.26043754124156	35.0	35.0	37.0	33.0	37.0
5	35.00730182901037	35.0	35.0	37.0	32.0	37.0
6	35.10990846488334	37.0	35.0	37.0	32.0	37.0
7	34.91704583523679	36.0	35.0	37.0	32.0	37.0
8	35.00070994704161	36.0	35.0	37.0	32.0	37.0
9	36.46660034177622	38.0	35.0	39.0	32.0	39.0
10	36.274821921053075	38.0	35.0	39.0	32.0	39.0
11	36.38437642759251	38.0	35.0	39.0	32.0	39.0
12	36.13304637666447	38.0	35.0	39.0	31.0	39.0
13	36.225810534152245	38.0	35.0	39.0	32.0	39.0
14	37.00909395462159	39.0	36.0	40.0	31.0	41.0
15	37.13565741163731	39.0	36.0	40.0	31.0	41.0
16	37.12302793428422	39.0	36.0	40.0	31.0	41.0
17	37.24575672977683	39.0	36.0	40.0	32.0	41.0
18	37.26419707967447	39.0	36.0	40.0	32.0	41.0
19	37.3665668409387	39.0	36.0	41.0	32.0	41.0
20	37.36182055056427	39.0	36.0	41.0	32.0	41.0
21	37.28342182291931	39.0	36.0	41.0	31.0	41.0
22	37.20957379490043	39.0	36.0	40.0	31.0	41.0
23	37.10268311253236	39.0	36.0	40.0	31.0	41.0
24	37.011669796795424	39.0	36.0	40.0	31.0	41.0
25	36.89142683112532	39.0	35.0	40.0	31.0	41.0
26	36.662802226621324	38.0	35.0	40.0	30.0	41.0
27	36.53712061993469	38.0	35.0	40.0	30.0	41.0
28	36.35210598446779	38.0	35.0	40.0	30.0	41.0
29	36.130307429402905	38.0	35.0	40.0	30.0	41.0
30	35.87722044566266	38.0	34.0	40.0	29.0	41.0
31	35.5901406019999	38.0	34.0	40.0	28.0	41.0
32	35.49886266348578	38.0	34.0	40.0	28.0	41.0
33	35.28898972979375	38.0	34.0	40.0	27.0	41.0
34	35.38796473952253	38.0	34.0	40.0	27.0	41.0
35	35.256707781330896	38.0	34.0	40.0	27.0	41.0
36	35.15338916806253	38.0	34.0	40.0	26.0	41.0
37	35.009114258159485	38.0	33.0	40.0	25.0	41.0
38	34.92164120264623	38.0	33.0	40.0	25.0	41.0
39	34.78061959629799	38.0	33.0	40.0	24.0	41.0
40	34.54478723584251	38.0	33.0	40.0	23.0	41.0
41	34.36816405258617	38.0	33.0	40.0	22.0	41.0
42	34.15013451093853	38.0	33.0	40.0	21.0	41.0
43	33.764327360709274	37.0	33.0	40.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	2.0
14	3.0
15	3.0
16	12.0
17	37.0
18	114.0
19	232.0
20	566.0
21	1096.0
22	2008.0
23	3403.0
24	5787.0
25	9227.0
26	13986.0
27	20527.0
28	29159.0
29	39148.0
30	50830.0
31	61539.0
32	74607.0
33	91071.0
34	109160.0
35	130624.0
36	161433.0
37	205936.0
38	238550.0
39	228512.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.680100840904856	22.364820736680034	15.069759572272135	21.88531885014297
2	18.12720166489011	23.979561105189244	36.9023569023569	20.990880327563744
3	19.547163426560413	25.1171006547891	34.59262643182241	20.74310948682808
4	12.830820770519264	15.911341217873881	36.78087406730623	34.47696394430063
5	11.34399269072636	38.97135509195811	35.81462869905081	13.870023518264723
6	31.142378559463985	37.160347190497944	12.859787151244436	18.83748709879363
7	23.6976803207959	35.22555538635941	21.454207062247264	19.622557230597433
8	26.362519669052332	34.4811600087982	19.212561122108863	19.943759200040606
9	23.1938818672487	14.692249124409928	23.966702197857977	38.1471668104834
10	15.792227128910547	27.47772532697156	34.22438793293065	22.505659611187248
11	30.967632776678002	22.30154137691826	25.817911104343267	20.91291474206047
12	21.341184034651373	27.29140652758743	31.86755325448793	19.499856183273266
13	32.2005989543678	21.385242711875875	28.255689220513343	18.158469113242983
14	19.6976803207959	23.96940933624351	29.27939360100164	27.053516741958955
15	20.562611034972843	30.007884540547856	28.054345803089518	21.37515862138978
16	20.26266010185608	29.620763751417016	28.275451330727712	21.841124815999187
17	20.321472683281726	26.618276568025312	27.76380217586248	25.29644857283048
18	23.042146760739726	24.820601323113888	32.109436069235066	20.027815846911324
19	21.562627954587754	24.03350083752094	33.57609596805577	20.827775239835542
20	23.40720437202849	23.016428946077188	33.80511987547163	19.771246806422685
21	21.840041960644978	24.396122024262727	34.62226959714397	19.141566417948326
22	19.154899074497063	24.698238668087917	33.95008713601678	22.19677512139824
23	21.142344720234167	25.292929292929294	35.58431890090182	17.980407085934726
24	21.032367223321994	24.80023010676277	32.01644586569209	22.150956804223135
25	20.48268277414006	26.01966059252491	33.29279393601002	20.20486269732501
26	20.298394328545083	27.02563321658799	33.05219701199601	19.62377544287092
27	18.05485339153681	27.292489382941643	32.801245283657344	21.851411941864203
28	19.485576028289596	26.0652758743211	32.780467996548396	21.668680100840902
29	19.580867299460262	26.00402686834848	31.493900478825104	22.921205353366158
30	20.05881258142565	26.2915249648918	34.35257093548551	19.297091518197046
31	21.844644095900378	25.753751924606195	33.708610392027474	18.69299358746595
32	20.862291254251055	25.938784833257195	31.838383838383837	21.360540074107913
33	20.247500126897112	25.93966465323249	32.96049269918617	20.852342520684232
34	18.584775730504376	27.311371673180716	34.065208195861466	20.038644400453446
35	18.03191039371944	30.562509517283388	31.782413752263	19.623166336734176
36	21.76870886418625	26.77576434360354	31.512376698306348	19.943150093903864
37	20.53093751586214	28.398490770350065	31.228330203204575	19.842241510583218
38	20.31091484357816	25.782921340710285	33.11012977344635	20.796034042265198
39	19.715818147978954	25.282439131685365	34.08896333519449	20.912779385141196
40	17.52418658951322	26.353992183137915	33.40141786372942	22.720403363619443
41	20.595367409437763	25.201766407796562	33.097338544574725	21.105527638190953
42	18.447929885115816	28.464951017714835	32.297649865489056	20.78946923168029
43	18.949630306414225	29.041774529211718	30.477099301219905	21.531495863154156
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	65.0
1	123.5
2	182.0
3	631.0
4	1080.0
5	1080.0
6	1909.5
7	2739.0
8	3168.5
9	3598.0
10	5599.5
11	7601.0
12	7601.0
13	14054.0
14	20507.0
15	34710.5
16	48914.0
17	47793.0
18	46672.0
19	46672.0
20	48264.5
21	49857.0
22	39563.5
23	29270.0
24	31682.0
25	34094.0
26	34094.0
27	35504.0
28	36914.0
29	37728.5
30	38543.0
31	41161.5
32	43780.0
33	43780.0
34	47584.5
35	51389.0
36	56100.5
37	60812.0
38	62813.5
39	64815.0
40	64815.0
41	67129.5
42	69444.0
43	72456.0
44	75468.0
45	86532.5
46	97597.0
47	97597.0
48	148413.5
49	199230.0
50	178955.0
51	158680.0
52	138581.5
53	118483.0
54	118483.0
55	97730.0
56	76977.0
57	59600.0
58	42223.0
59	35890.0
60	29557.0
61	29557.0
62	25489.0
63	21421.0
64	18590.0
65	15759.0
66	13414.5
67	11070.0
68	11070.0
69	9469.5
70	7869.0
71	6537.5
72	5206.0
73	4283.0
74	3360.0
75	3360.0
76	2755.5
77	2151.0
78	1605.0
79	1059.0
80	838.0
81	617.0
82	617.0
83	471.0
84	325.0
85	236.0
86	147.0
87	101.5
88	56.0
89	56.0
90	44.5
91	33.0
92	20.5
93	8.0
94	5.0
95	2.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1477575.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.810893790226174
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.73051596301062	33.277128631519275
2	12.094347896085948	10.355396864827616
3	4.105221043172451	5.272445461939719
4	1.8246718297748556	3.12463327626036
5	0.9940045970013179	2.1277112614609996
6	0.6248218324496284	1.6049508664089318
7	0.41634865760465606	1.247698071229132
8	0.31109934114183074	1.0654752681465804
9	0.22223493547792772	0.8562668597300997
>10	1.4217940501534199	11.721750819554199
>50	0.1511237517648578	4.463311583482402
>100	0.08595527725558184	7.102327964757832
>500	0.007335696026091907	2.2831950753243238
>1k	0.008930412553503192	7.411730615149195
>5k	0.001116301569187899	3.532080866458727
>10k+	4.784149582233852E-4	4.553896513750512
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	23030	1.5586349254690963	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	22919	1.5511226164492495	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	20754	1.4045987513324196	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	7854	0.5315466219988834	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7717	0.5222746730284419	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	7663	0.5186200362079759	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	7383	0.4996700675092635	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	7135	0.48288580951897536	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	7000	0.47374921746781046	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	6984	0.4726663621135983	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	4705	0.31842715259800686	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	4366	0.2954841547806372	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	4077	0.27592507994518045	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	3884	0.26286313723499655	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	3443	0.2330169365345245	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	3149	0.21311946940087645	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	2937	0.1987716359575656	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2884	0.1951846775967379	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	2756	0.1865218347630408	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	2694	0.18232577026546876	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2613	0.1768438150347698	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	2565	0.1735952489721334	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	2440	0.16513544151735107	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	2361	0.15978884320592862	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG	2278	0.15417153105595316	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	2083	0.14097423142649274	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	1966	0.13305585164881648	No Hit
GGGGAGAAGAGTAAAATGCAGCAACGCACATGCGAGGGTCCAA	1963	0.1328528162699017	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1921	0.13001032096509485	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCCT	1889	0.12784461025667054	No Hit
GTATTGGACCCTCGCATGTGCGTTGCTGCATTTTACTCTTCTC	1875	0.12689711182173494	No Hit
CATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAAC	1819	0.12310711808199246	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTG	1816	0.12290408270307768	No Hit
CGCATGTGCGTTGCTGCATTTTACTCTTCTCCCCTCGGCAGCA	1779	0.12039997969646211	No Hit
TCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGAT	1774	0.12006158739827082	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCT	1756	0.11884337512478216	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAAAAAAAAA	1741	0.11782819823020828	No Hit
ATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGGA	1737	0.11755748439165525	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1685	0.1140382044904658	No Hit
CGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAG	1664	0.11261695683806236	No Hit
CCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTG	1637	0.11078963842782938	No Hit
TTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCC	1602	0.10842089234049032	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAAAA	1592	0.10774410774410774	No Hit
ACGCACATGCGAGGGTCCAAAAAAAAAAAAAAAAAAAAAAAAA	1560	0.10557839703568346	No Hit
TTGCTGCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAAC	1541	0.10429250630255656	No Hit
CTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTG	1487	0.1006378694820906	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	1.3535691927651726E-4	0.0	0.0	0.0	0.0
9	1.3535691927651726E-4	0.0	0.0	1.3535691927651726E-4	0.0
10	1.3535691927651726E-4	0.0	0.0	4.060707578295518E-4	0.0
11	1.3535691927651726E-4	0.0	0.0	8.798199752973622E-4	0.0
12	1.3535691927651726E-4	0.0	0.0	0.00148892611204169	0.0
13	1.3535691927651726E-4	0.0	0.0	0.0018949968698712418	0.0
14	1.3535691927651726E-4	0.0	0.0	0.0023687460873390524	0.0
15	1.3535691927651726E-4	0.0	0.0	0.0033839229819129318	0.0
16	1.3535691927651726E-4	0.0	0.0	0.0048051706343163626	0.0
17	1.3535691927651726E-4	0.0	0.0	0.006564810584911087	0.0
18	1.3535691927651726E-4	0.0	0.0	0.0077830228583997425	0.0
19	2.707138385530345E-4	0.0	0.0	0.00988105510718576	0.0
20	2.707138385530345E-4	0.0	0.0	0.01576908109571426	0.0
21	2.707138385530345E-4	0.0	0.0	0.02835727458843037	0.0
22	2.707138385530345E-4	0.0	0.0	0.0438556418455916	0.0
23	2.707138385530345E-4	0.0	0.0	0.05265384159856522	0.0
24	2.707138385530345E-4	0.0	0.0	0.06530971355091958	0.0
25	2.707138385530345E-4	0.0	0.0	0.07241595181293674	0.0
26	2.707138385530345E-4	0.0	0.0	0.08520718068456762	0.0
27	2.707138385530345E-4	0.0	0.0	0.1874693331979764	0.0
28	2.707138385530345E-4	0.0	0.0	0.31111787895707493	0.0
29	2.707138385530345E-4	0.0	0.0	0.450873898110079	0.0
30	3.383922981912932E-4	0.0	0.0	0.7022993756662098	0.0
31	4.060707578295518E-4	0.0	0.0	1.0049574471685025	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5735	0.0	29.903227	1
GTATTGG	585	0.0	25.615385	1
CGAATTA	55	1.903136E-5	23.545454	15
ATTGGAC	655	0.0	22.59542	3
TTGGACC	1010	0.0	21.064358	4
TGGACCC	1090	0.0	19.518349	5
TATAGAG	105	2.26064E-8	19.38095	5
GGACCCT	1090	0.0	19.009174	6
TATTGGA	820	0.0	18.725609	2
ACGTTTA	60	9.238516E-4	18.5	26
GACCCTC	1145	0.0	18.25764	7
ATAGAGC	75	2.06777E-4	17.266666	6
CCCCGAA	75	2.06777E-4	17.266666	12
CCGAATT	75	2.06777E-4	17.266666	14
ACTTACC	110	7.8113044E-7	16.818182	8
GTATCAA	10340	0.0	16.639263	2
ATACCCT	610	0.0	16.37705	37
AAGACGT	80	3.3833826E-4	16.1875	5
GTAGTGT	80	3.3833826E-4	16.1875	6
GATACCT	795	0.0	16.056602	36
>>END_MODULE