FastQCFastQC Report
Wed 25 May 2016
SRR2088314_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088314_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1629568
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT212311.3028606354567591No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT209141.2834076270520776No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT179321.100414342942424No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72140.442694014610007No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA62590.3840895255675124No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG62090.3810212277118844No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA58350.35807035975178697No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG58320.3578862618804493No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG57920.35543162359594693No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA54300.3332171471212002No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA39090.2398795263529966No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA32820.20140307124342158No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA32190.19753701594533032No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC32120.19710745424554235No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA28850.1770407862697353No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT26920.16519715654701123No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA26350.16169929699159533No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG22720.13942345455973607No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG21890.1343300801193936No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA21540.132182271620454No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG21040.129113973764826No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT19760.12125913125441835No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC19640.12052273976906763No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG19480.11954088445526667No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19480.11954088445526667No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC18570.11395658235802371No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT17150.10524261644804021No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT16960.10407666326290159No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57900.027.670121
ACGTTTA951.0913936E-1125.31578626
GTATTGG5500.022.5363621
ATTGGAC6100.021.2295073
TTGGACC11100.018.3333344
GGACCCT11100.017.6666666
CGGCTTA752.0679343E-417.26666630
TGGACCC11800.017.0889845
AGGACGT650.001580317417.0769235
ACGACAT650.001580317417.07692327
TCACGTT1554.0199666E-1016.70967924
GTATCAA99000.016.2202032
TATACCG1151.2428973E-616.0869565
GACCCTC12000.016.0333337
TATTGGA8050.015.8571432
GCACTAG700.002593116415.8571431
TTAACGG1455.349284E-815.31034535
AGACGGC855.3658604E-415.2352936
TAATACT3150.014.682544
CTTAACG908.276629E-414.38888930
Produced by FastQC (version 0.11.5)