##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088314_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1629568
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.95948742243343	31.0	31.0	33.0	28.0	34.0
2	31.28874094238473	31.0	31.0	34.0	28.0	34.0
3	31.350674534600582	31.0	31.0	34.0	28.0	34.0
4	35.23752000530202	35.0	35.0	37.0	33.0	37.0
5	34.96433349206661	35.0	35.0	37.0	32.0	37.0
6	35.058260226023094	36.0	35.0	37.0	32.0	37.0
7	34.871900405506246	35.0	35.0	37.0	32.0	37.0
8	34.93448999980363	36.0	35.0	37.0	32.0	37.0
9	36.410908903463984	38.0	35.0	39.0	32.0	39.0
10	36.19775609241222	38.0	35.0	39.0	32.0	39.0
11	36.323150061856886	38.0	35.0	39.0	32.0	39.0
12	36.067393321420155	38.0	35.0	39.0	31.0	39.0
13	36.1464461746917	38.0	35.0	39.0	31.0	39.0
14	36.91778864091587	39.0	36.0	40.0	31.0	41.0
15	37.073312067983665	39.0	36.0	40.0	31.0	41.0
16	37.06701346614563	39.0	36.0	40.0	31.0	41.0
17	37.17094039647318	39.0	36.0	40.0	31.0	41.0
18	37.17906770383316	39.0	36.0	40.0	31.0	41.0
19	37.27904328018223	39.0	36.0	40.0	31.0	41.0
20	37.26654058008012	39.0	36.0	40.0	31.0	41.0
21	37.18777921510486	39.0	36.0	40.0	31.0	41.0
22	37.11321712257482	39.0	36.0	40.0	31.0	41.0
23	36.99506004045244	39.0	36.0	40.0	31.0	41.0
24	36.908691137773936	39.0	35.0	40.0	31.0	41.0
25	36.791192512371374	38.0	35.0	40.0	30.0	41.0
26	36.56612979636321	38.0	35.0	40.0	30.0	41.0
27	36.438179321145235	38.0	35.0	40.0	30.0	41.0
28	36.266620355824365	38.0	35.0	40.0	30.0	41.0
29	36.03290688084204	38.0	35.0	40.0	29.0	41.0
30	35.80285695349933	38.0	34.0	40.0	29.0	41.0
31	35.56961170076978	38.0	34.0	40.0	28.0	41.0
32	35.444606177833634	38.0	34.0	40.0	27.0	41.0
33	35.25578558243657	38.0	34.0	40.0	27.0	41.0
34	35.38089297286152	38.0	34.0	40.0	27.0	41.0
35	35.28939817178541	38.0	34.0	40.0	27.0	41.0
36	35.2122360036525	38.0	34.0	40.0	26.0	41.0
37	35.0924981344749	38.0	34.0	40.0	26.0	41.0
38	35.04533901009347	38.0	33.0	40.0	25.0	41.0
39	34.92675727554788	38.0	33.0	40.0	25.0	41.0
40	34.726733711020344	38.0	33.0	40.0	24.0	41.0
41	34.59389175536093	38.0	33.0	40.0	23.0	41.0
42	34.4244124823266	38.0	33.0	40.0	23.0	41.0
43	34.03703865073443	38.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	2.0
15	10.0
16	15.0
17	42.0
18	107.0
19	265.0
20	595.0
21	1229.0
22	2203.0
23	3951.0
24	6411.0
25	10203.0
26	15761.0
27	22768.0
28	32389.0
29	43304.0
30	55752.0
31	68128.0
32	83477.0
33	101063.0
34	121628.0
35	145073.0
36	177991.0
37	225755.0
38	265952.0
39	245492.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.37542465242322	22.425759465085225	14.697699022072108	22.501116860419447
2	18.23820791768125	23.973654367292436	36.21831061974707	21.56982709527924
3	19.57396070615034	25.149242007697747	33.7310256460608	21.545771640091115
4	13.155940715576154	16.370841842746053	36.75753328489514	33.71568415678266
5	11.84939812269264	38.761193150577334	35.608762567748016	13.780646158982012
6	31.190106825858145	37.49687033618726	13.593418623831594	17.71960421412301
7	24.225991182939282	34.51515984604509	21.916360655093868	19.342488315921766
8	26.154355019244363	34.64163508365407	19.650299956798367	19.553709940303197
9	23.68676851779122	14.702240102898436	23.475485527452676	38.13550585185767
10	15.812718462807322	27.639288449454085	34.393041591391096	22.154951496347497
11	31.636789627680468	22.72890729322127	24.94839123006834	20.685911849029928
12	21.040852544968974	27.33012675752101	31.754428167465242	19.874592530044772
13	31.425383905427694	21.569213435708114	27.700777138480877	19.30462552038332
14	19.86906959390464	23.72745414735685	29.034811680150813	27.368664578587698
15	21.276068258581414	29.91375628387401	27.277658864189775	21.532516593354803
16	20.766853546461395	29.206820457937315	27.94096349461943	22.085362500981855
17	20.72168820202655	27.107245601288195	27.745635653130158	24.425430543555102
18	22.743696488885398	25.377216538370906	31.405255871494774	20.473831101248923
19	21.88365259995287	24.84419183489121	32.52782332495484	20.744332240201082
20	23.07525675516456	24.106450298484017	32.68412241772052	20.1341705286309
21	21.8481217107847	25.160533343806456	33.23641603173356	19.754928913675283
22	19.748853683921137	25.553950494855076	32.68375422197785	22.013441599245937
23	21.32393370512921	25.780452242557537	33.823688241300765	19.071925811012488
24	21.14198364229047	25.641151519912025	31.157153306888695	22.059711530908803
25	20.859147945958682	26.553233740476003	32.123728497368624	20.463889816196684
26	20.63362805357003	27.194446626345144	31.97037497054434	20.201550349540494
27	19.163361087110204	27.39437691461786	31.671093197706384	21.771168800565547
28	20.105512626659337	26.532430681014844	31.741664048385832	21.62039264393999
29	20.205539136752808	26.33925064802451	30.931756146414262	22.52345406880842
30	20.534153837090567	26.725181152305392	32.95493038645825	19.785734624145785
31	21.806515591862382	26.34047796716676	32.42386939360616	19.4291370473647
32	20.86172531615741	26.58882599560129	31.10450131568612	21.44494737255518
33	20.489724884141072	26.36796991595319	31.954051625952403	21.188253573953343
34	19.334510741497134	27.1771414264394	32.87840703793889	20.60994079412458
35	18.931152305396274	29.615517732306966	31.198575327939675	20.25475463435708
36	21.794549230225435	26.842635594218834	30.915800997564997	20.44701417799073
37	20.760164657136123	27.787303138009584	30.791289470583617	20.661242734270676
38	20.58674446233603	25.870905663341446	32.20933400754065	21.33301586678187
39	20.0173911122457	25.44600777629408	33.09914038567277	21.437460725787446
40	18.412732699709373	26.168285091508913	32.74585657057576	22.673125638205953
41	20.51531448825701	25.197782479773778	32.610237805357	21.676665226612208
42	18.889914382216634	27.726182644725473	31.952517477024582	21.431385496033304
43	19.299838975728537	27.951579805199906	30.73072127091352	22.01785994815804
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	246.0
1	254.0
2	262.0
3	721.0
4	1180.0
5	1180.0
6	1982.0
7	2784.0
8	3166.5
9	3549.0
10	5568.0
11	7587.0
12	7587.0
13	13784.5
14	19982.0
15	33252.0
16	46522.0
17	44881.5
18	43241.0
19	43241.0
20	45824.0
21	48407.0
22	40043.0
23	31679.0
24	34248.0
25	36817.0
26	36817.0
27	39417.0
28	42017.0
29	44146.0
30	46275.0
31	49279.5
32	52284.0
33	52284.0
34	56752.5
35	61221.0
36	67289.5
37	73358.0
38	76463.0
39	79568.0
40	79568.0
41	81696.5
42	83825.0
43	87768.5
44	91712.0
45	100225.0
46	108738.0
47	108738.0
48	150707.0
49	192676.0
50	175319.0
51	157962.0
52	140050.5
53	122139.0
54	122139.0
55	103697.0
56	85255.0
57	70053.5
58	54852.0
59	47768.0
60	40684.0
61	40684.0
62	35587.5
63	30491.0
64	26631.0
65	22771.0
66	18839.0
67	14907.0
68	14907.0
69	12617.5
70	10328.0
71	8593.5
72	6859.0
73	5590.0
74	4321.0
75	4321.0
76	3386.5
77	2452.0
78	1858.5
79	1265.0
80	989.5
81	714.0
82	714.0
83	525.0
84	336.0
85	244.5
86	153.0
87	115.0
88	77.0
89	77.0
90	61.0
91	45.0
92	32.0
93	19.0
94	11.0
95	3.0
96	3.0
97	3.5
98	4.0
99	2.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1629568.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.386236541600404
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.06613730201481	36.99260448082328
2	12.307658890938738	11.664272709587086
3	4.033734451697087	5.734304846223629
4	1.8026298652655943	3.4167938076971485
5	0.9380352758056959	2.2224980731847044
6	0.5601450636114429	1.5925899889140984
7	0.3774865367848845	1.2521366426350655
8	0.2746422188579326	1.0411408917689586
9	0.20011958689687787	0.853462267317253
>10	1.223463425275381	11.020860525422263
>50	0.12786840799576135	4.156284853905501
>100	0.07488029643579862	6.621974116337356
>500	0.005619239327342818	1.9429995925856134
>1k	0.006272639249126866	5.131668937042093
>5k	9.14759890497668E-4	2.6361805034248698
>10k+	3.920399530704291E-4	3.720227763131143
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	21231	1.3028606354567591	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	20914	1.2834076270520776	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	17932	1.100414342942424	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7214	0.442694014610007	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	6259	0.3840895255675124	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	6209	0.3810212277118844	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	5835	0.35807035975178697	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	5832	0.3578862618804493	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	5792	0.35543162359594693	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5430	0.3332171471212002	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	3909	0.2398795263529966	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3282	0.20140307124342158	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3219	0.19753701594533032	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	3212	0.19710745424554235	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	2885	0.1770407862697353	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2692	0.16519715654701123	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	2635	0.16169929699159533	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	2272	0.13942345455973607	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	2189	0.1343300801193936	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2154	0.132182271620454	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	2104	0.129113973764826	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	1976	0.12125913125441835	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	1964	0.12052273976906763	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG	1948	0.11954088445526667	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1948	0.11954088445526667	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	1857	0.11395658235802371	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	1715	0.10524261644804021	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTCTGT	1696	0.10407666326290159	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	6.13659571125599E-5	0.0
7	0.0	0.0	0.0	6.13659571125599E-5	0.0
8	6.13659571125599E-5	0.0	0.0	6.13659571125599E-5	0.0
9	6.13659571125599E-5	0.0	0.0	6.13659571125599E-5	0.0
10	6.13659571125599E-5	0.0	0.0	2.454638284502396E-4	0.0
11	6.13659571125599E-5	0.0	0.0	6.750255282381588E-4	0.0
12	6.13659571125599E-5	0.0	0.0	0.0012886850993637578	0.0
13	6.13659571125599E-5	0.0	0.0	0.0014727829707014374	0.0
14	6.13659571125599E-5	0.0	0.0	0.0021478084989395964	0.0
15	6.13659571125599E-5	0.0	0.0	0.0026387361558400753	0.0
16	6.13659571125599E-5	0.0	0.0	0.0031910297698531146	0.0
17	6.13659571125599E-5	0.0	0.0	0.004234251040766633	0.0
18	6.13659571125599E-5	0.0	0.0	0.004541080826329432	0.0
19	6.13659571125599E-5	0.0	0.0	0.00558430209724295	0.0
20	6.13659571125599E-5	0.0	0.0	0.010002651009347262	0.0
21	6.13659571125599E-5	0.0	0.0	0.01791885947686749	0.0
22	6.13659571125599E-5	0.0	0.0	0.03025341685649203	0.0
23	6.13659571125599E-5	0.0	0.0	0.037740063624224336	0.0
24	6.13659571125599E-5	0.0	0.0	0.04731315293378368	0.0
25	6.13659571125599E-5	0.0	0.0	0.055229361401303904	0.0
26	6.13659571125599E-5	0.0	0.0	0.06768665069515356	0.0
27	6.13659571125599E-5	0.0	0.0	0.16390847144764747	0.0
28	6.13659571125599E-5	0.0	0.0	0.283326623988689	0.0
29	6.13659571125599E-5	0.0	0.0	0.4219523211059618	0.0
30	6.13659571125599E-5	0.0	0.0	0.6498654858220093	0.0
31	6.13659571125599E-5	0.0	0.0	0.9570021011703715	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5790	0.0	27.67012	1
ACGTTTA	95	1.0913936E-11	25.315786	26
GTATTGG	550	0.0	22.536362	1
ATTGGAC	610	0.0	21.229507	3
TTGGACC	1110	0.0	18.333334	4
GGACCCT	1110	0.0	17.666666	6
CGGCTTA	75	2.0679343E-4	17.266666	30
TGGACCC	1180	0.0	17.088984	5
AGGACGT	65	0.0015803174	17.076923	5
ACGACAT	65	0.0015803174	17.076923	27
TCACGTT	155	4.0199666E-10	16.709679	24
GTATCAA	9900	0.0	16.220203	2
TATACCG	115	1.2428973E-6	16.086956	5
GACCCTC	1200	0.0	16.033333	7
TATTGGA	805	0.0	15.857143	2
GCACTAG	70	0.0025931164	15.857143	1
TTAACGG	145	5.349284E-8	15.310345	35
AGACGGC	85	5.3658604E-4	15.235293	6
TAATACT	315	0.0	14.68254	4
CTTAACG	90	8.276629E-4	14.388889	30
>>END_MODULE