FastQCFastQC Report
Wed 25 May 2016
SRR2088313_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088313_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1664002
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT204791.2307076553994525No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT202391.2162845958117838No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT173531.042847304270067No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69310.41652594167555085No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG59670.3585933189984147No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA57470.3453721810430516No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA55650.3344346941890695No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG55290.3322712352509192No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG55080.3310092175369982No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA50130.30126165713743136No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA38390.23070885732108495No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC32740.19675457120844805No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA30890.18563679610962006No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA30340.1823315116207793No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA26740.16069692223927615No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT26180.1573315416688201No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA26170.15727144558720482No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG21370.1284253264118673No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA21150.12710321261633098No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG21090.12674263612663927No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG20320.1221152378422622No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG19950.1198916828224966No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19540.11742774347626986No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT19240.11562486102781126No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC18440.11081717449858833No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC17390.10450708592898326No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT17380.10444698984736797No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51250.029.455611
ATTGGAC4900.021.1428593
GTATTGG5200.020.2788471
ATAGTCG656.904506E-519.92307730
TCTAGAC1457.2759576E-1219.137933
ACGTTTA1501.2732926E-1118.526
TAACGCG500.00703617418.520
ACTCACG701.2196135E-418.56
TTGGACC9300.017.7043024
TAAAGCG650.001580336217.0769234
GTATCAA90800.016.7477972
GACGTCG904.4478824E-516.44444536
GCGCATA803.383705E-416.187526
TAGTCGC803.383705E-416.187531
TATTGGA6350.016.0236232
GCGATAT700.002593147315.85714315
TGGACCC10750.015.8325595
GGACCCT10250.015.702446
GTCTAGA1954.1836756E-1115.1794871
TACCCGC750.004105433414.818
Produced by FastQC (version 0.11.5)