##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2088313_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1664002
Sequences flagged as poor quality	0
Sequence length	43
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.98871515779428	31.0	31.0	33.0	28.0	34.0
2	31.310756838032646	31.0	31.0	34.0	30.0	34.0
3	31.37498813102388	31.0	31.0	34.0	28.0	34.0
4	35.25850089122489	35.0	35.0	37.0	33.0	37.0
5	34.981070335252	35.0	35.0	37.0	32.0	37.0
6	35.07332863782616	36.0	35.0	37.0	32.0	37.0
7	34.890310227992515	36.0	35.0	37.0	32.0	37.0
8	34.95093755896928	36.0	35.0	37.0	32.0	37.0
9	36.43823505019826	38.0	35.0	39.0	32.0	39.0
10	36.224110908520544	38.0	35.0	39.0	32.0	39.0
11	36.34407711048424	38.0	35.0	39.0	32.0	39.0
12	36.09133702964299	38.0	35.0	39.0	31.0	39.0
13	36.17252563398361	38.0	35.0	39.0	32.0	39.0
14	36.964073961449564	39.0	36.0	40.0	31.0	41.0
15	37.11370178641612	39.0	36.0	40.0	31.0	41.0
16	37.10532198879569	39.0	36.0	40.0	31.0	41.0
17	37.19455204981725	39.0	36.0	40.0	31.0	41.0
18	37.195770798352406	39.0	36.0	40.0	31.0	41.0
19	37.28521600334615	39.0	36.0	40.0	31.0	41.0
20	37.28977729594075	39.0	36.0	40.0	31.0	41.0
21	37.21485611195179	39.0	36.0	40.0	31.0	41.0
22	37.137349594531734	39.0	36.0	40.0	31.0	41.0
23	37.022142401271154	39.0	36.0	40.0	31.0	41.0
24	36.93239431202607	39.0	35.0	40.0	31.0	41.0
25	36.82881991728375	39.0	35.0	40.0	31.0	41.0
26	36.60783460596802	38.0	35.0	40.0	30.0	41.0
27	36.46774402915381	38.0	35.0	40.0	30.0	41.0
28	36.30844794657699	38.0	35.0	40.0	30.0	41.0
29	36.087197611541335	38.0	35.0	40.0	30.0	41.0
30	35.86833248998499	38.0	34.0	40.0	29.0	41.0
31	35.63299803726198	38.0	34.0	40.0	28.0	41.0
32	35.51451921331825	38.0	34.0	40.0	28.0	41.0
33	35.331775442577595	38.0	34.0	40.0	27.0	41.0
34	35.46696818874016	38.0	34.0	40.0	27.0	41.0
35	35.393319238798995	38.0	34.0	40.0	27.0	41.0
36	35.325054897770556	38.0	34.0	40.0	27.0	41.0
37	35.204913816209356	38.0	34.0	40.0	26.0	41.0
38	35.155541880358314	38.0	34.0	40.0	26.0	41.0
39	35.0507096746278	38.0	34.0	40.0	25.0	41.0
40	34.85001039662212	38.0	33.0	40.0	24.0	41.0
41	34.722538795025486	38.0	33.0	40.0	24.0	41.0
42	34.553157388031984	38.0	33.0	40.0	23.0	41.0
43	34.17435676159043	38.0	33.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	6.0
16	18.0
17	39.0
18	112.0
19	263.0
20	627.0
21	1178.0
22	2198.0
23	3967.0
24	6364.0
25	10262.0
26	15604.0
27	22875.0
28	31945.0
29	42851.0
30	55712.0
31	68326.0
32	83338.0
33	102342.0
34	123864.0
35	147740.0
36	181573.0
37	229836.0
38	274968.0
39	257993.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.29165830329531	22.43458841996584	14.499021034830486	22.774732241908364
2	18.42744179394015	23.794021882185238	35.78096660941513	21.997569714459477
3	19.43254875895582	24.989753618084592	33.62465910497704	21.953038517982552
4	13.432736258730458	16.31007655038876	36.463057135748635	33.79413005513214
5	12.048603306967179	38.562934419549975	35.5681663844154	13.820295889067442
6	31.629228811023065	37.268164341148626	13.698841708122947	17.40376513970536
7	24.5199825480979	34.345571700034014	21.844024225932422	19.290421525935667
8	26.24389874531401	34.543588288956386	19.60454374453877	19.607969221190842
9	23.79834880006154	14.527206097108056	23.374911809000228	38.29953329383017
10	15.854968924316196	27.71991860586706	34.376220701657815	22.04889176815893
11	32.00392787989437	22.606943982038484	24.686268405927397	20.702859732139743
12	21.005563695235942	27.256818200939666	31.55254621088196	20.185071892942435
13	31.564926003694705	21.514577506517423	27.29365709896983	19.62683939081804
14	19.9993750007512	23.619262476847986	28.760602451198974	27.62076007120184
15	21.555202457689354	29.977968776479834	26.777071181404832	21.68975758442598
16	21.203039419423774	28.93067436217024	27.498765025522804	22.36752119288318
17	20.808809124027494	26.94924645523263	27.62442593218037	24.617518488559508
18	22.90868640782884	25.40159206539415	30.91564793792315	20.77407358885386
19	21.879180433677366	24.925450810756235	32.2355982745213	20.959770481045094
20	23.187291842197304	24.30447799942548	32.1502618386276	20.357968319749617
21	22.087533548637563	25.142878434040345	32.67267707610929	20.096910941212812
22	20.200636778080796	25.60597883896774	32.077725868117945	22.115658514833516
23	21.660130216189643	25.8633102604444	33.19034472314336	19.286214800222595
24	21.383027183861557	25.7115676543658	30.77977069739099	22.12563446438165
25	21.05646507636409	26.40147067130929	31.842629996838944	20.699434255487674
26	20.812354792842797	27.12003951918327	31.545214488924895	20.52239119904904
27	19.46223622327377	27.203633168710134	31.298459977812527	22.03567063020357
28	20.287595808178118	26.539150794289913	31.30891669601359	21.86433670151839
29	20.373172628398283	26.436807167299076	30.458497045075667	22.73152315922697
30	20.73555200053846	26.779835601159135	32.43583841846344	20.048773979838966
31	21.95964908696023	26.268598234857894	32.078987885831864	19.692764792350008
32	21.125996242792976	26.53608589412753	30.76510725347686	21.572810609602634
33	20.770948592609866	26.341975550510156	31.56402456247048	21.323051294409503
34	19.566743309202753	27.186806265857854	32.411319217164404	20.83513120777499
35	19.282368650999217	29.52316163081535	30.909998906251314	20.28447081193412
36	21.95111544337086	26.89167440904518	30.456994643035284	20.700215504548673
37	20.98254689597729	27.972081764324802	30.325985185113964	20.719386154583948
38	20.741020743965453	26.195100727042398	31.712882556631545	21.350995972360607
39	20.198833895632337	25.703695067674197	32.61600647114607	21.481464565547398
40	18.737717863319876	26.293417916565005	32.32039384568047	22.648470374434645
41	20.695888586672375	25.300330167872392	32.21720887354703	21.786572371908207
42	19.118186156026255	27.750687799654088	31.528688066480687	21.60243797783897
43	19.463858817477384	27.903692423446607	30.350504386412997	22.281944372663013
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	86.0
1	149.5
2	213.0
3	633.0
4	1053.0
5	1053.0
6	1889.5
7	2726.0
8	3098.5
9	3471.0
10	5416.0
11	7361.0
12	7361.0
13	13375.0
14	19389.0
15	32071.5
16	44754.0
17	43522.5
18	42291.0
19	42291.0
20	45173.5
21	48056.0
22	39800.5
23	31545.0
24	34577.5
25	37610.0
26	37610.0
27	40629.5
28	43649.0
29	45153.0
30	46657.0
31	49990.0
32	53323.0
33	53323.0
34	58615.0
35	63907.0
36	69584.5
37	75262.0
38	77896.0
39	80530.0
40	80530.0
41	83030.5
42	85531.0
43	88472.5
44	91414.0
45	100198.0
46	108982.0
47	108982.0
48	150528.5
49	192075.0
50	174339.5
51	156604.0
52	138859.0
53	121114.0
54	121114.0
55	105141.0
56	89168.0
57	75048.5
58	60929.0
59	54276.0
60	47623.0
61	47623.0
62	41299.0
63	34975.0
64	30288.5
65	25602.0
66	21410.5
67	17219.0
68	17219.0
69	14368.0
70	11517.0
71	9673.0
72	7829.0
73	6482.5
74	5136.0
75	5136.0
76	4050.5
77	2965.0
78	2312.0
79	1659.0
80	1284.0
81	909.0
82	909.0
83	682.0
84	455.0
85	340.0
86	225.0
87	166.5
88	108.0
89	108.0
90	80.5
91	53.0
92	36.5
93	20.0
94	12.5
95	5.0
96	5.0
97	3.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1664002.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.51940174686761
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.72807104874985	36.46072033427746
2	13.024982021785176	12.378787068778752
3	4.45618496810714	6.352657312735068
4	1.9504486540351522	3.7073661271093434
5	0.9948145652527157	2.363649649493962
6	0.5918163501698441	1.687365534245142
7	0.37978881613836213	1.2633136133132243
8	0.2739654499070372	1.0414939423115066
9	0.20510805679439725	0.8771950937099069
>10	1.1852109599869347	10.752353015968765
>50	0.12441919149875798	4.108088971044387
>100	0.0723288059725999	6.315251275142122
>500	0.005602860499875734	1.8995945612135652
>1k	0.005984873715776351	4.842141750626952
>5k	8.913641704347758E-4	2.4361378980810415
>10k+	3.820132159006182E-4	3.513883851948936
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	20479	1.2307076553994525	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	20239	1.2162845958117838	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	17353	1.042847304270067	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6931	0.41652594167555085	No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG	5967	0.3585933189984147	No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA	5747	0.3453721810430516	No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA	5565	0.3344346941890695	No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG	5529	0.3322712352509192	No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG	5508	0.3310092175369982	No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	5013	0.30126165713743136	No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA	3839	0.23070885732108495	No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC	3274	0.19675457120844805	No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA	3089	0.18563679610962006	No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA	3034	0.1823315116207793	No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA	2674	0.16069692223927615	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2618	0.1573315416688201	No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA	2617	0.15727144558720482	No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG	2137	0.1284253264118673	No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA	2115	0.12710321261633098	No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG	2109	0.12674263612663927	No Hit
ACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGG	2032	0.1221152378422622	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG	1995	0.1198916828224966	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1954	0.11742774347626986	No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT	1924	0.11562486102781126	No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC	1844	0.11081717449858833	No Hit
CTTGCTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGC	1739	0.10450708592898326	No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACTGT	1738	0.10444698984736797	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	6.009608161528652E-5	6.009608161528652E-5
6	6.009608161528652E-5	0.0	0.0	6.009608161528652E-5	6.009608161528652E-5
7	1.2019216323057304E-4	0.0	0.0	6.009608161528652E-5	6.009608161528652E-5
8	1.8028824484585956E-4	0.0	0.0	6.009608161528652E-5	6.009608161528652E-5
9	1.8028824484585956E-4	0.0	0.0	1.2019216323057304E-4	6.009608161528652E-5
10	1.8028824484585956E-4	0.0	0.0	1.8028824484585956E-4	6.009608161528652E-5
11	1.8028824484585956E-4	0.0	0.0	4.2067257130700564E-4	6.009608161528652E-5
12	1.8028824484585956E-4	0.0	0.0	6.610568977681517E-4	6.009608161528652E-5
13	2.4038432646114607E-4	0.0	0.0	8.413451426140113E-4	6.009608161528652E-5
14	2.4038432646114607E-4	0.0	0.0	0.001021633387459871	6.009608161528652E-5
15	2.4038432646114607E-4	0.0	0.0	0.0016826902852280225	6.009608161528652E-5
16	2.4038432646114607E-4	0.0	0.0	0.002283651101380888	1.2019216323057304E-4
17	2.4038432646114607E-4	0.0	0.0	0.0025841315094573204	1.2019216323057304E-4
18	2.4038432646114607E-4	0.0	0.0	0.003245188407225472	1.2019216323057304E-4
19	2.4038432646114607E-4	0.0	0.0	0.004206725713070057	1.2019216323057304E-4
20	2.4038432646114607E-4	0.0	0.0	0.006310088569605085	1.2019216323057304E-4
21	2.4038432646114607E-4	0.0	0.0	0.011959120241442018	1.8028824484585956E-4
22	3.605764896917191E-4	0.0	0.0	0.019471130443352833	1.8028824484585956E-4
23	3.605764896917191E-4	0.0	0.0	0.02536054644165091	1.8028824484585956E-4
24	3.605764896917191E-4	0.0	0.0	0.03179082717448657	1.8028824484585956E-4
25	3.605764896917191E-4	0.0	0.0	0.03731966668309293	1.8028824484585956E-4
26	3.605764896917191E-4	0.0	0.0	0.048978306516458514	1.8028824484585956E-4
27	3.605764896917191E-4	0.0	0.0	0.14603347832514624	1.8028824484585956E-4
28	3.605764896917191E-4	0.0	0.0	0.26225930016911037	1.8028824484585956E-4
29	3.605764896917191E-4	0.0	0.0	0.39212693253974457	1.8028824484585956E-4
30	3.605764896917191E-4	0.0	0.0	0.6067300399879327	1.8028824484585956E-4
31	3.605764896917191E-4	0.0	0.0	0.8835325919079424	1.8028824484585956E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	5125	0.0	29.45561	1
ATTGGAC	490	0.0	21.142859	3
GTATTGG	520	0.0	20.278847	1
ATAGTCG	65	6.904506E-5	19.923077	30
TCTAGAC	145	7.2759576E-12	19.13793	3
ACGTTTA	150	1.2732926E-11	18.5	26
TAACGCG	50	0.007036174	18.5	20
ACTCACG	70	1.2196135E-4	18.5	6
TTGGACC	930	0.0	17.704302	4
TAAAGCG	65	0.0015803362	17.076923	4
GTATCAA	9080	0.0	16.747797	2
GACGTCG	90	4.4478824E-5	16.444445	36
GCGCATA	80	3.383705E-4	16.1875	26
TAGTCGC	80	3.383705E-4	16.1875	31
TATTGGA	635	0.0	16.023623	2
GCGATAT	70	0.0025931473	15.857143	15
TGGACCC	1075	0.0	15.832559	5
GGACCCT	1025	0.0	15.70244	6
GTCTAGA	195	4.1836756E-11	15.179487	1
TACCCGC	75	0.0041054334	14.8	18
>>END_MODULE