FastQCFastQC Report
Wed 25 May 2016
SRR2088312_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2088312_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences474008
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT53191.1221329597812695No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT52431.106099475114344No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT44350.935638217076505No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17590.37109078327791933No Hit
ATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAA15060.3177161566893386No Hit
TATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAGG14510.30611297699616885No Hit
GAGTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAG14490.3056910431891445No Hit
GGTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTA14400.3037923410575349No Hit
GTATCAACGCAGAGTACGGGCTTGCTACACGATTCAGACTTAG13960.2945097973029991No Hit
ATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA13610.2871259556800729No Hit
TTCCTAAGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGA9880.20843530067003088No Hit
CTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGTCTGAA7760.16371031712544937No Hit
CGCTCGAACATTCCTAAGTCTGAATCGTGTAGCAAGCCCGTAC7670.16181161499383975No Hit
GACTTAGGAATGTTCGAGCGTTGCTGCCGAGGGGAGAAGAGTA7570.159701945958718No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7230.15252907123930398No Hit
CGGCAGCAACGCTCGAACATTCCTAAGTCTGAATCGTGTAGCA6740.14219169296720732No Hit
GAGTAAAATGCAGCAACGCACATGCGAGGGTCCAAAAAAAAAA6320.13333108301969587No Hit
CTACACGATTCAGACTTAGGAATGTTCGAGCGTTGCTGCCGAG5530.11666469764223389No Hit
TTTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTA5440.11476599551062429No Hit
GTACGGGCTTGCTACACGATTCAGACTTAGGAATGTTCGAGCG5400.11392212789657558No Hit
AGTCTGAATCGTGTAGCAAGCCCGTACTCTGCGTTGATACCTG5080.10717118698418593No Hit
TTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCCTAAGT4840.10210798129989367No Hit
GCATTTTACTCTTCTCCCCTCGGCAGCAACGCTCGAACATTCC4820.10168604749286933No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTC352.3853698E-531.7142855
GGTATCA14050.028.9679721
CGGGTCT405.932878E-527.7500026
ACCGTCG358.8616763E-426.4285723
GTGGCGT400.00192952223.1250026
CCGTCGG400.00192952223.12500224
TCACGTA400.00192952223.12500225
CAGACCG652.6773268E-622.7692320
GGCGTGA502.698858E-422.1999998
GACCGTC450.003822541620.55555522
TACGGGT909.4487405E-820.5555554
TACCTGC450.003822541620.5555556
CTAGCCG450.003822541620.55555510
TACTCCG555.13697E-420.1818185
CTCTAGG701.2172804E-418.51
CTAATAC801.6134063E-518.53
CTCCTAA500.007028934518.4999981
GCTCGAG500.007028934518.49999829
GCGTCGG500.007028934518.4999989
TAGCTCG500.007028934518.49999827
Produced by FastQC (version 0.11.5)